##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BcnI BglI
BglII BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsiYI BslI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BssHII Bst1107I BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoT22I FseI
HaeII HapII HgaI Hin1I HincII
HindII HpaI HpaII HphI KasI
KpnI KspI MfeI MluI MroI
MslI MspA1I MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PstI PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AclI 1130 AACGTT AA^CGTT
AflII 830 CTTAAG C^TTAAG
AlwNI 536 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 115 TGCGCA TGC^GCA
AvrII 774 CCTAGG C^CTAGG
BalI 595 TGGCCA TGG^CCA
BanII 1044 GAGCTC G[GA]GC[CT]^C
BbvCI 822 GCTGAGG CCTCAGC(-5/-2)
BclI 437 TGATCA T^GATCA
BfrI 830 CTTAAG C^TTAAG
BlnI 774 CCTAGG C^CTAGG
BlpI 1046 GCTCAGC GC^T[ACGT]AGC
BpmI 862 CTGGAG CTGGAG(16/14)
Bpu1102I 1046 GCTCAGC GC^T[ACGT]AGC
BsaBI 438 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 502 CTCCTC GAGGAG(10/8)
BsmFI 675 GTCCC GGGAC(10/14)
BsmI 894 GAATGC GAATGC(1/-1)
Bsp1407I 1106 TGTACA T^GTACA
BsrBI 70 CCGCTC CCGCTC(-3/-3)
BsrDI 545 GCAATG GCAATG(2/0)
BsrGI 1106 TGTACA T^GTACA
BssKI 563 CCAGG ^CC[ACGT]GG
BssSI 710 CACGAG CACGAG(-5/-1)
BstAPI 898 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 256 TTCGAA TT^CGAA
BstNI 563 CCAGG CC^[AT]GG
BstYI 926 AGATCC [GA]^GATC[CT]
CelII 1046 GCTCAGC GC^T[ACGT]AGC
CfoI 116 GCGC GCG^C
Cfr13I 280 GGGCC G^G[ACGT]CC
DsaI 598 CCATGG C^C[GA][CT]GG
EcoRII 563 CCAGG ^CC[AT]GG
EcoRV 3 GATATC GAT^ATC
FauI 69 CCCGC CCCGC(4/6)
FbaI 437 TGATCA T^GATCA
FspI 115 TGCGCA TGC^GCA
HhaI 116 GCGC GCG^C
HinP1I 116 GCGC G^CGC
MaeII 1131 ACGT A^CGT
MamI 438 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 926 AGATCC [GA]^GATC[CT]
MluNI 595 TGGCCA TGG^CCA
MscI 595 TGGCCA TGG^CCA
MvaI 563 CCAGG CC^[AT]GG
NcoI 598 CCATGG C^CATGG
NlaIV 647 GGAGCC GG[ACGT]^[ACGT]CC
NspV 256 TTCGAA TT^CGAA
PleI 987 GACTC GAGTC(4/5)
Psp1406I 1130 AACGTT AA^CGTT
SacI 1044 GAGCTC GAGCT^C
SapI 390 GCTCTTC GCTCTTC(1/4)
Sau96I 280 GGGCC G^G[ACGT]CC
ScrFI 563 CCAGG CC^[ACGT]GG
SfcI 397 CTACAG C^T[GA][CT]AG
SfuI 256 TTCGAA TT^CGAA
SpeI 30 ACTAGT A^CTAGT
SspBI 1106 TGTACA T^GTACA
TaqI 257 TCGA T^CGA
TthHB8I 257 TCGA T^CGA
XhoII 926 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 70 CCGC CCGC(-3/-1)
53 GCGG
AcsI 127 AAATTC [GA]^AATT[CT]
418 GAATTT
AfaI 318 GTAC GT^AC
1107 GTAC
AlwI 498 GATCC GGATC(4/5)
927 GATCC
AocI 339 CCTAAGG CC^T[ACGT]AGG
744 CCTGAGG
ApoI 127 AAATTC [GA]^AATT[CT]
418 GAATTT
Asp700I 186 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
765 GAAAGAGTTC
AspHI 334 GAGCAC G[AT]GC[AT]^C
1044 GAGCTC
BmyI 334 GAGCAC G[AGT]GC[ACT]^C
1044 GAGCTC
BsiHKAI 334 GAGCAC G[AT]GC[AT]^C
1044 GAGCTC
BsmAI 635 GAGAC GTCTC(1/5)
944 GTCTC
Bsp1286I 334 GAGCAC G[AGT]GC[ACT]^C
1044 GAGCTC
Bsu36I 339 CCTAAGG CC^T[ACGT]AGG
744 CCTGAGG
Cac8I 68 GCCCGC GC[ACGT]^[ACGT]GC
898 GCAGGC
EaeI 290 TGGCCG [CT]^GGCC[GA]
595 TGGCCA
EarI 391 CTCTTC CTCTTC(1/4)
989 CTCTTC
Eco81I 339 CCTAAGG CC^T[ACGT]AGG
744 CCTGAGG
HindIII 189 AAGCTT A^AGCTT
734 AAGCTT
Ksp632I 391 CTCTTC CTCTTC(1/4)
989 CTCTTC
RsaI 318 GTAC GT^AC
1107 GTAC
SmlI 830 CTTAAG C^T[CT][GA]AG
1119 CTCAAG
XmnI 186 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
765 GAAAGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI BbvI BfaI BsaJI BsrI
BstF5I DdeI DpnII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MwoI NdeII NlaIII Sau3AI SfaNI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI