################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII BamHI BanI BbeI BbrPI BbsI BcgI BciVI BcnI BglI BglII BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrFI BssHII Bst1107I BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FseI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII HphI KasI KpnI KspI MfeI MluI MroI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AclI 1130 AACGTT AA^CGTT AflII 830 CTTAAG C^TTAAG AlwNI 536 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG AviII 115 TGCGCA TGC^GCA AvrII 774 CCTAGG C^CTAGG BalI 595 TGGCCA TGG^CCA BanII 1044 GAGCTC G[GA]GC[CT]^C BbvCI 822 GCTGAGG CCTCAGC(-5/-2) BclI 437 TGATCA T^GATCA BfrI 830 CTTAAG C^TTAAG BlnI 774 CCTAGG C^CTAGG BlpI 1046 GCTCAGC GC^T[ACGT]AGC BpmI 862 CTGGAG CTGGAG(16/14) Bpu1102I 1046 GCTCAGC GC^T[ACGT]AGC BsaBI 438 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 502 CTCCTC GAGGAG(10/8) BsmFI 675 GTCCC GGGAC(10/14) BsmI 894 GAATGC GAATGC(1/-1) Bsp1407I 1106 TGTACA T^GTACA BsrBI 70 CCGCTC CCGCTC(-3/-3) BsrDI 545 GCAATG GCAATG(2/0) BsrGI 1106 TGTACA T^GTACA BssKI 563 CCAGG ^CC[ACGT]GG BssSI 710 CACGAG CACGAG(-5/-1) BstAPI 898 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 256 TTCGAA TT^CGAA BstNI 563 CCAGG CC^[AT]GG BstYI 926 AGATCC [GA]^GATC[CT] CelII 1046 GCTCAGC GC^T[ACGT]AGC CfoI 116 GCGC GCG^C Cfr13I 280 GGGCC G^G[ACGT]CC DsaI 598 CCATGG C^C[GA][CT]GG EcoRII 563 CCAGG ^CC[AT]GG EcoRV 3 GATATC GAT^ATC FauI 69 CCCGC CCCGC(4/6) FbaI 437 TGATCA T^GATCA FspI 115 TGCGCA TGC^GCA HhaI 116 GCGC GCG^C HinP1I 116 GCGC G^CGC MaeII 1131 ACGT A^CGT MamI 438 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 926 AGATCC [GA]^GATC[CT] MluNI 595 TGGCCA TGG^CCA MscI 595 TGGCCA TGG^CCA MvaI 563 CCAGG CC^[AT]GG NcoI 598 CCATGG C^CATGG NlaIV 647 GGAGCC GG[ACGT]^[ACGT]CC NspV 256 TTCGAA TT^CGAA PleI 987 GACTC GAGTC(4/5) Psp1406I 1130 AACGTT AA^CGTT SacI 1044 GAGCTC GAGCT^C SapI 390 GCTCTTC GCTCTTC(1/4) Sau96I 280 GGGCC G^G[ACGT]CC ScrFI 563 CCAGG CC^[ACGT]GG SfcI 397 CTACAG C^T[GA][CT]AG SfuI 256 TTCGAA TT^CGAA SpeI 30 ACTAGT A^CTAGT SspBI 1106 TGTACA T^GTACA TaqI 257 TCGA T^CGA TthHB8I 257 TCGA T^CGA XhoII 926 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 70 CCGC CCGC(-3/-1) 53 GCGG AcsI 127 AAATTC [GA]^AATT[CT] 418 GAATTT AfaI 318 GTAC GT^AC 1107 GTAC AlwI 498 GATCC GGATC(4/5) 927 GATCC AocI 339 CCTAAGG CC^T[ACGT]AGG 744 CCTGAGG ApoI 127 AAATTC [GA]^AATT[CT] 418 GAATTT Asp700I 186 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 765 GAAAGAGTTC AspHI 334 GAGCAC G[AT]GC[AT]^C 1044 GAGCTC BmyI 334 GAGCAC G[AGT]GC[ACT]^C 1044 GAGCTC BsiHKAI 334 GAGCAC G[AT]GC[AT]^C 1044 GAGCTC BsmAI 635 GAGAC GTCTC(1/5) 944 GTCTC Bsp1286I 334 GAGCAC G[AGT]GC[ACT]^C 1044 GAGCTC Bsu36I 339 CCTAAGG CC^T[ACGT]AGG 744 CCTGAGG Cac8I 68 GCCCGC GC[ACGT]^[ACGT]GC 898 GCAGGC EaeI 290 TGGCCG [CT]^GGCC[GA] 595 TGGCCA EarI 391 CTCTTC CTCTTC(1/4) 989 CTCTTC Eco81I 339 CCTAAGG CC^T[ACGT]AGG 744 CCTGAGG HindIII 189 AAGCTT A^AGCTT 734 AAGCTT Ksp632I 391 CTCTTC CTCTTC(1/4) 989 CTCTTC RsaI 318 GTAC GT^AC 1107 GTAC SmlI 830 CTTAAG C^T[CT][GA]AG 1119 CTCAAG XmnI 186 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 765 GAAAGAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AluI BbvI BfaI BsaJI BsrI BstF5I DdeI DpnII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII Sau3AI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI