################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI ApaI AscI Asp700I Asp718I AspI AviII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BsaAI BsaBI BseAI BsiWI BsmBI BspDI BspEI BspHI BsrDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraIII DrdI EcoNI EcoRV FseI FspI HindIII KasI KpnI MamI MfeI MluI MroI MslI MunI NarI NheI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SwaI Tth111I XcmI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AcsI 2142 GAATTC [GA]^AATT[CT] AflIII 685 ACATGT A^C[GA][CT]GT AhdI 6 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 862 GTGCAC G^TGCAC AocI 2281 CCTCAGG CC^T[ACGT]AGG Aor51HI 1492 AGCGCT AGC^GCT ApaLI 862 GTGCAC G^TGCAC ApoI 2142 GAATTC [GA]^AATT[CT] AseI 1160 ATTAAT AT^TAAT AsnI 1160 ATTAAT AT^TAAT AspEI 6 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 1247 CCTAGG C^CTAGG BalI 1051 TGGCCA TGG^CCA BanII 1828 GAGCCC G[GA]GC[CT]^C BbsI 502 GTCTTC GAAGAC(2/6) BclI 1222 TGATCA T^GATCA BlnI 1247 CCTAGG C^CTAGG BlpI 2040 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2040 GCTGAGC GC^T[ACGT]AGC BpuAI 502 GTCTTC GAAGAC(2/6) BsaWI 1590 TCCGGT [AT]^CCGG[AT] Bsp1407I 591 TGTACA T^GTACA BspLU11I 685 ACATGT A^CATGT BsrGI 591 TGTACA T^GTACA BssSI 2009 CTCGTG CACGAG(-5/-1) BstEII 2361 GGTCACC G^GT[ACGT]ACC BstPI 2361 GGTCACC G^GT[ACGT]ACC BstXI 1606 CCAGCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1647 AGATCC [GA]^GATC[CT] Bsu36I 2281 CCTCAGG CC^T[ACGT]AGG CelII 2040 GCTGAGC GC^T[ACGT]AGC Eam1105I 6 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1337 GAAGAG CTCTTC(1/4) Eco47III 1492 AGCGCT AGC^GCT Eco81I 2281 CCTCAGG CC^T[ACGT]AGG EcoO65I 2361 GGTCACC G^GT[ACGT]ACC EcoRI 2142 GAATTC G^AATTC FbaI 1222 TGATCA T^GATCA HincII 1067 GTTAAC GT[CT]^[GA]AC HindII 1067 GTTAAC GT[CT]^[GA]AC HpaI 1067 GTTAAC GTT^AAC Ksp632I 1337 GAAGAG CTCTTC(1/4) MflI 1647 AGATCC [GA]^GATC[CT] MluNI 1051 TGGCCA TGG^CCA MscI 1051 TGGCCA TGG^CCA NcoI 1711 CCATGG C^CATGG NdeI 935 CATATG CA^TATG NotI 20 GCGGCCGC GC^GGCCGC PaeR7I 63 CTCGAG C^TCGAG PshBI 1160 ATTAAT AT^TAAT PstI 1499 CTGCAG CTGCA^G PvuII 1275 CAGCTG CAG^CTG SapI 1337 GAAGAGC GCTCTTC(1/4) ScaI 572 AGTACT AGT^ACT SpeI 779 ACTAGT A^CTAGT SspBI 591 TGTACA T^GTACA StuI 805 AGGCCT AGG^CCT TfiI 1237 GAATC G^A[AT]TC XbaI 1568 TCTAGA T^CTAGA XhoI 63 CTCGAG C^TCGAG XhoII 1647 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 29 GACGCC G[GA]^CG[CT]C 1678 GGCGTC AlwI 1648 GATCC GGATC(4/5) 1904 GATCC BsaHI 29 GACGCC G[GA]^CG[CT]C 1678 GGCGTC BseRI 75 CTCCTC GAGGAG(10/8) 215 GAGGAG BsrBI 124 CCGCTC CCGCTC(-3/-3) 66 GAGCGG BsrFI 204 GCCGGC [GA]^CCGG[CT] 1730 GCCGGC BstAPI 628 GCAAGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1501 GCAGGTGCTGC Cfr10I 204 GCCGGC [GA]^CCGG[CT] 1730 GCCGGC DraI 887 TTTAAA TTT^AAA 2375 TTTAAA EagI 21 CGGCCG C^GGCCG 86 CGGCCG EclXI 21 CGGCCG C^GGCCG 86 CGGCCG Eco52I 21 CGGCCG C^GGCCG 86 CGGCCG EcoT22I 532 ATGCAT ATGCA^T 1318 ATGCAT HaeII 181 AGCGCC [GA]GCGC^[CT] 1492 AGCGCT Hin1I 29 GACGCC G[GA]^CG[CT]C 1678 GGCGTC MaeII 223 ACGT A^CGT 672 ACGT NaeI 204 GCCGGC GCC^GGC 1730 GCCGGC NgoMIV 204 GCCGGC G^CCGGC 1730 GCCGGC NsiI 532 ATGCAT ATGCA^T 1318 ATGCAT PflMI 367 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1994 CCATCCATTGG PpuMI 540 AGGACCT [GA]G^G[AT]CC[CT] 1413 AGGACCC SfcI 557 CTGTAG C^T[GA][CT]AG 1499 CTGCAG SmaI 190 CCCGGG CCC^GGG 1390 CCCGGG Van91I 367 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1994 CCATCCATTGG XmaCI 190 CCCGGG C^CCGGG 1390 CCCGGG XmaI 190 CCCGGG C^CCGGG 1390 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaI AvaII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KspI MaeI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SmlI SspI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I