##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI ApaI AscI Asp700I
Asp718I AspI AviII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BsaAI BsaBI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BsrDI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraIII DrdI
EcoNI EcoRV FseI FspI HindIII
KasI KpnI MamI MfeI MluI
MroI MslI MunI NarI NheI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
Tth111I XcmI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AcsI 2142 GAATTC [GA]^AATT[CT]
AflIII 685 ACATGT A^C[GA][CT]GT
AhdI 6 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 862 GTGCAC G^TGCAC
AocI 2281 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1492 AGCGCT AGC^GCT
ApaLI 862 GTGCAC G^TGCAC
ApoI 2142 GAATTC [GA]^AATT[CT]
AseI 1160 ATTAAT AT^TAAT
AsnI 1160 ATTAAT AT^TAAT
AspEI 6 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1247 CCTAGG C^CTAGG
BalI 1051 TGGCCA TGG^CCA
BanII 1828 GAGCCC G[GA]GC[CT]^C
BbsI 502 GTCTTC GAAGAC(2/6)
BclI 1222 TGATCA T^GATCA
BlnI 1247 CCTAGG C^CTAGG
BlpI 2040 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2040 GCTGAGC GC^T[ACGT]AGC
BpuAI 502 GTCTTC GAAGAC(2/6)
BsaWI 1590 TCCGGT [AT]^CCGG[AT]
Bsp1407I 591 TGTACA T^GTACA
BspLU11I 685 ACATGT A^CATGT
BsrGI 591 TGTACA T^GTACA
BssSI 2009 CTCGTG CACGAG(-5/-1)
BstEII 2361 GGTCACC G^GT[ACGT]ACC
BstPI 2361 GGTCACC G^GT[ACGT]ACC
BstXI 1606 CCAGCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1647 AGATCC [GA]^GATC[CT]
Bsu36I 2281 CCTCAGG CC^T[ACGT]AGG
CelII 2040 GCTGAGC GC^T[ACGT]AGC
Eam1105I 6 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1337 GAAGAG CTCTTC(1/4)
Eco47III 1492 AGCGCT AGC^GCT
Eco81I 2281 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2361 GGTCACC G^GT[ACGT]ACC
EcoRI 2142 GAATTC G^AATTC
FbaI 1222 TGATCA T^GATCA
HincII 1067 GTTAAC GT[CT]^[GA]AC
HindII 1067 GTTAAC GT[CT]^[GA]AC
HpaI 1067 GTTAAC GTT^AAC
Ksp632I 1337 GAAGAG CTCTTC(1/4)
MflI 1647 AGATCC [GA]^GATC[CT]
MluNI 1051 TGGCCA TGG^CCA
MscI 1051 TGGCCA TGG^CCA
NcoI 1711 CCATGG C^CATGG
NdeI 935 CATATG CA^TATG
NotI 20 GCGGCCGC GC^GGCCGC
PaeR7I 63 CTCGAG C^TCGAG
PshBI 1160 ATTAAT AT^TAAT
PstI 1499 CTGCAG CTGCA^G
PvuII 1275 CAGCTG CAG^CTG
SapI 1337 GAAGAGC GCTCTTC(1/4)
ScaI 572 AGTACT AGT^ACT
SpeI 779 ACTAGT A^CTAGT
SspBI 591 TGTACA T^GTACA
StuI 805 AGGCCT AGG^CCT
TfiI 1237 GAATC G^A[AT]TC
XbaI 1568 TCTAGA T^CTAGA
XhoI 63 CTCGAG C^TCGAG
XhoII 1647 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 29 GACGCC G[GA]^CG[CT]C
1678 GGCGTC
AlwI 1648 GATCC GGATC(4/5)
1904 GATCC
BsaHI 29 GACGCC G[GA]^CG[CT]C
1678 GGCGTC
BseRI 75 CTCCTC GAGGAG(10/8)
215 GAGGAG
BsrBI 124 CCGCTC CCGCTC(-3/-3)
66 GAGCGG
BsrFI 204 GCCGGC [GA]^CCGG[CT]
1730 GCCGGC
BstAPI 628 GCAAGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1501 GCAGGTGCTGC
Cfr10I 204 GCCGGC [GA]^CCGG[CT]
1730 GCCGGC
DraI 887 TTTAAA TTT^AAA
2375 TTTAAA
EagI 21 CGGCCG C^GGCCG
86 CGGCCG
EclXI 21 CGGCCG C^GGCCG
86 CGGCCG
Eco52I 21 CGGCCG C^GGCCG
86 CGGCCG
EcoT22I 532 ATGCAT ATGCA^T
1318 ATGCAT
HaeII 181 AGCGCC [GA]GCGC^[CT]
1492 AGCGCT
Hin1I 29 GACGCC G[GA]^CG[CT]C
1678 GGCGTC
MaeII 223 ACGT A^CGT
672 ACGT
NaeI 204 GCCGGC GCC^GGC
1730 GCCGGC
NgoMIV 204 GCCGGC G^CCGGC
1730 GCCGGC
NsiI 532 ATGCAT ATGCA^T
1318 ATGCAT
PflMI 367 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1994 CCATCCATTGG
PpuMI 540 AGGACCT [GA]G^G[AT]CC[CT]
1413 AGGACCC
SfcI 557 CTGTAG C^T[GA][CT]AG
1499 CTGCAG
SmaI 190 CCCGGG CCC^GGG
1390 CCCGGG
Van91I 367 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1994 CCATCCATTGG
XmaCI 190 CCCGGG C^CCGGG
1390 CCCGGG
XmaI 190 CCCGGG C^CCGGG
1390 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI KspI
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SmlI SspI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I