################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AvaI AviII AvrII BamHI BbeI BcgI BclI BfrI BglI BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI PaeR7I PinAI PmeI PshAI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI TfiI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcsI 2072 AAATTT [GA]^AATT[CT] AcyI 1701 GGCGTC G[GA]^CG[CT]C AlwNI 1141 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1298 CCTTAGG CC^T[ACGT]AGG ApaI 420 GGGCCC GGGCC^C ApoI 2072 AAATTT [GA]^AATT[CT] AspI 579 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 353 TGGCCA TGG^CCA BanI 339 GGTGCC G^G[CT][GA]CC BbrPI 1609 CACGTG CAC^GTG BbsI 157 GAAGAC GAAGAC(2/6) BcnI 600 CCCGG CC^[GC]GG BglII 1745 AGATCT A^GATCT BpuAI 157 GAAGAC GAAGAC(2/6) BsaAI 1609 CACGTG [CT]AC^GT[GA] BsaHI 1701 GGCGTC G[GA]^CG[CT]C BsaI 103 GAGACC GGTCTC(1/5) Bsp1407I 1928 TGTACA T^GTACA BspHI 1125 TCATGA T^CATGA BsrDI 1031 GCAATG GCAATG(2/0) BsrGI 1928 TGTACA T^GTACA BssSI 2065 CTCGTG CACGAG(-5/-1) BstBI 1577 TTCGAA TT^CGAA BstXI 1044 CCAGGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1298 CCTTAGG CC^T[ACGT]AGG EaeI 353 TGGCCA [CT]^GGCC[GA] Eco81I 1298 CCTTAGG CC^T[ACGT]AGG EcoRV 616 GATATC GAT^ATC FauI 1381 GCGGG CCCGC(4/6) HapII 601 CCGG C^CGG Hin1I 1701 GGCGTC G[GA]^CG[CT]C HincII 570 GTTGAC GT[CT]^[GA]AC HindII 570 GTTGAC GT[CT]^[GA]AC HpaII 601 CCGG C^CGG MluNI 353 TGGCCA TGG^CCA MscI 353 TGGCCA TGG^CCA MslI 1126 CATGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 601 CCGG C^CGG NciI 600 CCCGG CC^[GC]GG NheI 1243 GCTAGC G^CTAGC NspV 1577 TTCGAA TT^CGAA PacI 1859 TTAATTAA TTAAT^TAA PflFI 579 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 750 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1609 CACGTG CAC^GTG PmlI 1609 CACGTG CAC^GTG RcaI 1125 TCATGA T^CATGA SfuI 1577 TTCGAA TT^CGAA SphI 1131 GCATGC GCATG^C SspBI 1928 TGTACA T^GTACA Tth111I 579 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 750 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1013 CCATCTGTCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 212 CCGC CCGC(-3/-1) 1381 GCGG Aor51HI 532 AGCGCT AGC^GCT 1390 AGCGCT Asp700I 1336 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1591 GAAGGCATTC BbvCI 453 CCTCAGC CCTCAGC(-5/-2) 763 GCTGAGG BsgI 1583 CTGCAC GTGCAG(16/14) 1788 GTGCAG BsmAI 103 GAGAC GTCTC(1/5) 1654 GTCTC BsmI 963 GCATTC GAATGC(1/-1) 1595 GCATTC CfoI 533 GCGC GCG^C 1391 GCGC DraI 1258 TTTAAA TTT^AAA 2116 TTTAAA DsaI 215 CCATGG C^C[GA][CT]GG 1022 CCATGG Eco47III 532 AGCGCT AGC^GCT 1390 AGCGCT HaeII 532 AGCGCT [GA]GCGC^[CT] 1390 AGCGCT HgaI 364 GCGTC GACGC(5/10) 1702 GCGTC HhaI 533 GCGC GCG^C 1391 GCGC HinP1I 533 GCGC G^CGC 1391 GCGC HinfI 592 GAGTC G^A[ACGT]TC 1618 GACTC NcoI 215 CCATGG C^CATGG 1022 CCATGG NspI 1131 GCATGC [GA]CATG^[CT] 1311 GCATGT PleI 1618 GACTC GAGTC(4/5) 592 GAGTC SfcI 431 CTGTAG C^T[GA][CT]AG 926 CTACAG SmlI 887 CTCAAG C^T[CT][GA]AG 983 CTCAAG SspI 1338 AATATT AAT^ATT 1546 AATATT TaqI 98 TCGA T^CGA 1578 TCGA TthHB8I 98 TCGA T^CGA 1578 TCGA XbaI 1800 TCTAGA T^CTAGA 2080 TCTAGA XmnI 1336 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1591 GAAGGCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AfaI AluI AlwI AseI AsnI AspHI AvaII BanII BbvI BciVI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PpuMI PshBI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII