##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I ApaLI AscI Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BcgI BclI BfrI BglI BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PaeR7I PinAI
PmeI PshAI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI TfiI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcsI 2072 AAATTT [GA]^AATT[CT]
AcyI 1701 GGCGTC G[GA]^CG[CT]C
AlwNI 1141 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1298 CCTTAGG CC^T[ACGT]AGG
ApaI 420 GGGCCC GGGCC^C
ApoI 2072 AAATTT [GA]^AATT[CT]
AspI 579 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 353 TGGCCA TGG^CCA
BanI 339 GGTGCC G^G[CT][GA]CC
BbrPI 1609 CACGTG CAC^GTG
BbsI 157 GAAGAC GAAGAC(2/6)
BcnI 600 CCCGG CC^[GC]GG
BglII 1745 AGATCT A^GATCT
BpuAI 157 GAAGAC GAAGAC(2/6)
BsaAI 1609 CACGTG [CT]AC^GT[GA]
BsaHI 1701 GGCGTC G[GA]^CG[CT]C
BsaI 103 GAGACC GGTCTC(1/5)
Bsp1407I 1928 TGTACA T^GTACA
BspHI 1125 TCATGA T^CATGA
BsrDI 1031 GCAATG GCAATG(2/0)
BsrGI 1928 TGTACA T^GTACA
BssSI 2065 CTCGTG CACGAG(-5/-1)
BstBI 1577 TTCGAA TT^CGAA
BstXI 1044 CCAGGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1298 CCTTAGG CC^T[ACGT]AGG
EaeI 353 TGGCCA [CT]^GGCC[GA]
Eco81I 1298 CCTTAGG CC^T[ACGT]AGG
EcoRV 616 GATATC GAT^ATC
FauI 1381 GCGGG CCCGC(4/6)
HapII 601 CCGG C^CGG
Hin1I 1701 GGCGTC G[GA]^CG[CT]C
HincII 570 GTTGAC GT[CT]^[GA]AC
HindII 570 GTTGAC GT[CT]^[GA]AC
HpaII 601 CCGG C^CGG
MluNI 353 TGGCCA TGG^CCA
MscI 353 TGGCCA TGG^CCA
MslI 1126 CATGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 601 CCGG C^CGG
NciI 600 CCCGG CC^[GC]GG
NheI 1243 GCTAGC G^CTAGC
NspV 1577 TTCGAA TT^CGAA
PacI 1859 TTAATTAA TTAAT^TAA
PflFI 579 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 750 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1609 CACGTG CAC^GTG
PmlI 1609 CACGTG CAC^GTG
RcaI 1125 TCATGA T^CATGA
SfuI 1577 TTCGAA TT^CGAA
SphI 1131 GCATGC GCATG^C
SspBI 1928 TGTACA T^GTACA
Tth111I 579 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 750 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1013 CCATCTGTCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 212 CCGC CCGC(-3/-1)
1381 GCGG
Aor51HI 532 AGCGCT AGC^GCT
1390 AGCGCT
Asp700I 1336 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1591 GAAGGCATTC
BbvCI 453 CCTCAGC CCTCAGC(-5/-2)
763 GCTGAGG
BsgI 1583 CTGCAC GTGCAG(16/14)
1788 GTGCAG
BsmAI 103 GAGAC GTCTC(1/5)
1654 GTCTC
BsmI 963 GCATTC GAATGC(1/-1)
1595 GCATTC
CfoI 533 GCGC GCG^C
1391 GCGC
DraI 1258 TTTAAA TTT^AAA
2116 TTTAAA
DsaI 215 CCATGG C^C[GA][CT]GG
1022 CCATGG
Eco47III 532 AGCGCT AGC^GCT
1390 AGCGCT
HaeII 532 AGCGCT [GA]GCGC^[CT]
1390 AGCGCT
HgaI 364 GCGTC GACGC(5/10)
1702 GCGTC
HhaI 533 GCGC GCG^C
1391 GCGC
HinP1I 533 GCGC G^CGC
1391 GCGC
HinfI 592 GAGTC G^A[ACGT]TC
1618 GACTC
NcoI 215 CCATGG C^CATGG
1022 CCATGG
NspI 1131 GCATGC [GA]CATG^[CT]
1311 GCATGT
PleI 1618 GACTC GAGTC(4/5)
592 GAGTC
SfcI 431 CTGTAG C^T[GA][CT]AG
926 CTACAG
SmlI 887 CTCAAG C^T[CT][GA]AG
983 CTCAAG
SspI 1338 AATATT AAT^ATT
1546 AATATT
TaqI 98 TCGA T^CGA
1578 TCGA
TthHB8I 98 TCGA T^CGA
1578 TCGA
XbaI 1800 TCTAGA T^CTAGA
2080 TCTAGA
XmnI 1336 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1591 GAAGGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AfaI AluI AlwI AseI AsnI
AspHI AvaII BanII BbvI BciVI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PpuMI PshBI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII