##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AvrII BanII BbeI BbvCI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BseAI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstZ17I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI Eco47III EcoO109I EcoRI
EcoRV EcoT22I FbaI FseI HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
PacI PflMI PinAI PmeI PpuMI
PshAI PshBI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 82 CGCG CG^CG
AclI 519 AACGTT AA^CGTT
AcsI 1224 AAATTT [GA]^AATT[CT]
AcyI 1142 GACGCC G[GA]^CG[CT]C
AhdI 1842 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2139 GTGCAC G^TGCAC
AocI 769 CCTTAGG CC^T[ACGT]AGG
ApaLI 2139 GTGCAC G^TGCAC
ApoI 1224 AAATTT [GA]^AATT[CT]
AspEI 1842 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 722 TGCGCA TGC^GCA
BglII 1858 AGATCT A^GATCT
BsaHI 1142 GACGCC G[GA]^CG[CT]C
BsaI 135 GAGACC GGTCTC(1/5)
BsaWI 455 ACCGGA [AT]^CCGG[AT]
BsmBI 1961 GAGACG CGTCTC(1/5)
BspLU11I 2171 ACATGT A^CATGT
BstBI 682 TTCGAA TT^CGAA
BstEII 1737 GGTAACC G^GT[ACGT]ACC
BstPI 1737 GGTAACC G^GT[ACGT]ACC
BstUI 82 CGCG CG^CG
Bsu36I 769 CCTTAGG CC^T[ACGT]AGG
DraI 2213 TTTAAA TTT^AAA
EagI 1272 CGGCCG C^GGCCG
Eam1105I 1842 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1272 CGGCCG C^GGCCG
Eco52I 1272 CGGCCG C^GGCCG
Eco81I 769 CCTTAGG CC^T[ACGT]AGG
EcoNI 1664 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1737 GGTAACC G^GT[ACGT]ACC
FspI 722 TGCGCA TGC^GCA
HaeII 1467 AGCGCC [GA]GCGC^[CT]
Hin1I 1142 GACGCC G[GA]^CG[CT]C
HincII 1717 GTCAAC GT[CT]^[GA]AC
HindII 1717 GTCAAC GT[CT]^[GA]AC
HindIII 12 AAGCTT A^AGCTT
MvnI 82 CGCG CG^CG
NcoI 845 CCATGG C^CATGG
NheI 1881 GCTAGC G^CTAGC
NspV 682 TTCGAA TT^CGAA
PaeR7I 1969 CTCGAG C^TCGAG
Psp1406I 519 AACGTT AA^CGTT
PvuI 527 CGATCG CGAT^CG
SfuI 682 TTCGAA TT^CGAA
SnaBI 198 TACGTA TAC^GTA
XcmI 615 CCAGATGAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1969 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 299 ACACGT A^C[GA][CT]GT
2171 ACATGT
Asp700I 440 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2245 GAAACTTTTC
AspHI 459 GAGCAC G[AT]GC[AT]^C
2139 GTGCAC
AspI 953 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1975 GACAGTGTC
AvaI 1481 CCCGAG C^[CT]CG[GA]G
1969 CTCGAG
BalI 490 TGGCCA TGG^CCA
1312 TGGCCA
BbrPI 300 CACGTG CAC^GTG
1330 CACGTG
BcgI 803 CGACACGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
517 GCAACGTTCTCG
BseRI 1488 GAGGAG GAGGAG(10/8)
1671 GAGGAG
BsiHKAI 459 GAGCAC G[AT]GC[AT]^C
2139 GTGCAC
BsoBI 1481 CCCGAG C^[CT]CG[GA]G
1969 CTCGAG
BspMI 793 ACCTGC ACCTGC(4/8)
2284 GCAGGT
BstXI 268 CCATTACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
688 CCAACTGTGTGG
DsaI 845 CCATGG C^C[GA][CT]GG
921 CCGTGG
FauI 1472 CCCGC CCCGC(4/6)
1 GCGGG
HgaI 80 GACGC GACGC(5/10)
1142 GACGC
MluNI 490 TGGCCA TGG^CCA
1312 TGGCCA
MscI 490 TGGCCA TGG^CCA
1312 TGGCCA
MseI 1203 TTAA T^TAA
2214 TTAA
NspI 2147 ACATGC [GA]CATG^[CT]
2171 ACATGT
PflFI 953 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1975 GACAGTGTC
PmaCI 300 CACGTG CAC^GTG
1330 CACGTG
PmlI 300 CACGTG CAC^GTG
1330 CACGTG
SapI 411 GAAGAGC GCTCTTC(1/4)
309 GCTCTTC
Tru9I 1203 TTAA T^TAA
2214 TTAA
Tth111I 953 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1975 GACAGTGTC
XmnI 440 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2245 GAAACTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AvaII BamHI BanI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BsiEI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII