##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspHI AspI BcgI
BciVI BclI BfrI BglII BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BspDI BspEI BspHI BsrBI BsrDI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI FbaI FseI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SfiI SfuI SgrAI
SmaI SpeI SphI SspI StuI
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 2217 GGTACC G^GTACC
AccI 1361 GTAGAC GT^[AC][GT]AC
AhdI 615 GACCGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 520 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 533 CCTTAGG CC^T[ACGT]AGG
Asp700I 1990 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2217 GGTACC G^GTACC
AspEI 615 GACCGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 347 TGCGCA TGC^GCA
AvrII 1999 CCTAGG C^CTAGG
BamHI 729 GGATCC G^GATCC
BbeI 1958 GGCGCC GGCGC^C
BbrPI 1037 CACGTG CAC^GTG
BbvCI 771 GCTGAGG CCTCAGC(-5/-2)
BglI 1772 GCCTTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1999 CCTAGG C^CTAGG
BlpI 2004 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2004 GCTCAGC GC^T[ACGT]AGC
BsaI 2039 GAGACC GGTCTC(1/5)
BsmBI 2713 GAGACG CGTCTC(1/5)
BsmI 1543 GCATTC GAATGC(1/-1)
Bsp1407I 1033 TGTACA T^GTACA
BspLU11I 763 ACATGT A^CATGT
BsrFI 616 ACCGGC [GA]^CCGG[CT]
BsrGI 1033 TGTACA T^GTACA
BssSI 649 CACGAG CACGAG(-5/-1)
Bsu36I 533 CCTTAGG CC^T[ACGT]AGG
CelII 2004 GCTCAGC GC^T[ACGT]AGC
Cfr10I 616 ACCGGC [GA]^CCGG[CT]
DraI 1142 TTTAAA TTT^AAA
DraIII 1652 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 615 GACCGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 533 CCTTAGG CC^T[ACGT]AGG
EcoNI 335 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 201 GATATC GAT^ATC
EcoT22I 1541 ATGCAT ATGCA^T
FauI 1045 CCCGC CCCGC(4/6)
FspI 347 TGCGCA TGC^GCA
HgaI 891 GACGC GACGC(5/10)
HindIII 1755 AAGCTT A^AGCTT
KasI 1958 GGCGCC G^GCGCC
KpnI 2217 GGTACC GGTAC^C
NarI 1958 GGCGCC GG^CGCC
NsiI 1541 ATGCAT ATGCA^T
PaeR7I 1834 CTCGAG C^TCGAG
PmaCI 1037 CACGTG CAC^GTG
PmlI 1037 CACGTG CAC^GTG
PshAI 2538 GACTAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 284 CCTGCAGG CCTGCA^GG
SfoI 1958 GGCGCC GGC^GCC
SnaBI 1358 TACGTA TAC^GTA
Sse8387I 284 CCTGCAGG CCTGCA^GG
SspBI 1033 TGTACA T^GTACA
TaqI 1835 TCGA T^CGA
TthHB8I 1835 TCGA T^CGA
XhoI 1834 CTCGAG C^TCGAG
XmnI 1990 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 891 GACGCC G[GA]^CG[CT]C
1958 GGCGCC
AflIII 763 ACATGT A^C[GA][CT]GT
1036 ACACGT
BalI 1183 TGGCCA TGG^CCA
1717 TGGCCA
BcnI 956 CCCGG CC^[GC]GG
990 CCGGG
BsaAI 1037 CACGTG [CT]AC^GT[GA]
1358 TACGTA
BsaHI 891 GACGCC G[GA]^CG[CT]C
1958 GGCGCC
BstXI 1861 CCAGGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2553 CCATGGCGCTGG
BstYI 352 AGATCC [GA]^GATC[CT]
729 GGATCC
DsaI 1720 CCATGG C^C[GA][CT]GG
2553 CCATGG
Hin1I 891 GACGCC G[GA]^CG[CT]C
1958 GGCGCC
HincII 2186 GTTAAC GT[CT]^[GA]AC
2775 GTTAAC
HindII 2186 GTTAAC GT[CT]^[GA]AC
2775 GTTAAC
HpaI 2186 GTTAAC GTT^AAC
2775 GTTAAC
MflI 352 AGATCC [GA]^GATC[CT]
729 GGATCC
MluNI 1183 TGGCCA TGG^CCA
1717 TGGCCA
MscI 1183 TGGCCA TGG^CCA
1717 TGGCCA
MslI 309 CACTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1782 CATTGTAGTG
NciI 956 CCCGG CC^[GC]GG
990 CCGGG
NcoI 1720 CCATGG C^CATGG
2553 CCATGG
NspI 763 ACATGT [GA]CATG^[CT]
1539 ACATGC
PflMI 638 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2169 CCAGGCATTGG
PvuII 362 CAGCTG CAG^CTG
1107 CAGCTG
SexAI 418 ACCAGGT A^CC[AT]GGT
1224 ACCTGGT
SmlI 1645 CTTGAG C^T[CT][GA]AG
1834 CTCGAG
Van91I 638 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2169 CCAGGCATTGG
XbaI 2081 TCTAGA T^CTAGA
2799 TCTAGA
XcmI 82 CCAAAAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2618 CCATTGTAGTCATGG
XhoII 352 AGATCC [GA]^GATC[CT]
729 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI AvaI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI