##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AocI ApaI
ApaLI AscI Asp718I AspHI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaWI BseAI
BsiHKAI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I ClaI
CpoI DrdI Eco81I EcoNI EcoRI
EcoRV EcoT22I FseI FspI HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PaeR7I
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SacII
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
TfiI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 229 GTCGAC GT^[AC][GT]AC
AcyI 10 GGCGCC G[GA]^CG[CT]C
AhdI 1078 GACTATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 155 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 96 AGCGCT AGC^GCT
Asp700I 29 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1078 GACTATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1242 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1897 TGGCCA TGG^CCA
BbeI 10 GGCGCC GGCGC^C
BclI 729 TGATCA T^GATCA
BglI 176 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 92 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 92 GCTGAGC GC^T[ACGT]AGC
BsaHI 10 GGCGCC G[GA]^CG[CT]C
BseRI 835 GAGGAG GAGGAG(10/8)
BsgI 2009 CTGCAC GTGCAG(16/14)
BsmI 483 GAATGC GAATGC(1/-1)
Bsp1407I 1360 TGTACA T^GTACA
BsrDI 884 GCAATG GCAATG(2/0)
BsrFI 1554 GCCGGT [GA]^CCGG[CT]
BsrGI 1360 TGTACA T^GTACA
BstAPI 1160 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 470 GGTTACC G^GT[ACGT]ACC
BstPI 470 GGTTACC G^GT[ACGT]ACC
CelII 92 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1554 GCCGGT [GA]^CCGG[CT]
DraI 2070 TTTAAA TTT^AAA
DraIII 2054 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1078 GACTATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 96 AGCGCT AGC^GCT
EcoO65I 470 GGTTACC G^GT[ACGT]ACC
FbaI 729 TGATCA T^GATCA
HgaI 135 GCGTC GACGC(5/10)
Hin1I 10 GGCGCC G[GA]^CG[CT]C
HincII 229 GTCGAC GT[CT]^[GA]AC
HindII 229 GTCGAC GT[CT]^[GA]AC
HindIII 798 AAGCTT A^AGCTT
KasI 10 GGCGCC G^GCGCC
MluNI 1897 TGGCCA TGG^CCA
MscI 1897 TGGCCA TGG^CCA
MslI 875 CACATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 10 GGCGCC GG^CGCC
NdeI 1545 CATATG CA^TATG
PacI 1505 TTAATTAA TTAAT^TAA
PflFI 1242 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 843 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 749 CTGCAG CTGCA^G
PvuII 1655 CAGCTG CAG^CTG
SalI 229 GTCGAC G^TCGAC
SexAI 1136 ACCAGGT A^CC[AT]GGT
SfoI 10 GGCGCC GGC^GCC
SmlI 1311 CTCAAG C^T[CT][GA]AG
SphI 960 GCATGC GCATG^C
SspBI 1360 TGTACA T^GTACA
SspI 633 AATATT AAT^ATT
StuI 1120 AGGCCT AGG^CCT
Tth111I 1242 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 435 TCTAGA T^CTAGA
XcmI 644 CCATGGACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 29 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AseI 454 ATTAAT AT^TAAT
1504 ATTAAT
AsnI 454 ATTAAT AT^TAAT
1504 ATTAAT
AvaI 1331 CCCGAG C^[CT]CG[GA]G
1977 CCCGAG
BanII 1648 GGGCTC G[GA]GC[CT]^C
1928 GAGCCC
BfaI 368 CTAG C^TAG
436 CTAG
BsmFI 1100 GGGAC GGGAC(10/14)
137 GTCCC
BsoBI 1331 CCCGAG C^[CT]CG[GA]G
1977 CCCGAG
BspHI 405 TCATGA T^CATGA
732 TCATGA
DraII 1100 GGGACCT [GA]G^G[ACGT]CC[CT]
1246 GGGTCCT
EagI 84 CGGCCG C^GGCCG
116 CGGCCG
EclXI 84 CGGCCG C^GGCCG
116 CGGCCG
Eco52I 84 CGGCCG C^GGCCG
116 CGGCCG
EcoO109I 1100 GGGACCT [GA]G^G[ACGT]CC[CT]
1246 GGGTCCT
MaeI 368 CTAG C^TAG
436 CTAG
MaeII 239 ACGT A^CGT
1909 ACGT
NspI 932 ACATGC [GA]CATG^[CT]
960 GCATGC
PpuMI 1100 GGGACCT [GA]G^G[AT]CC[CT]
1246 GGGTCCT
PshBI 454 ATTAAT AT^TAAT
1504 ATTAAT
RcaI 405 TCATGA T^CATGA
732 TCATGA
SapI 976 GAAGAGC GCTCTTC(1/4)
1844 GCTCTTC
SfaNI 1190 GCATC GCATC(5/9)
1943 GCATC
SfcI 749 CTGCAG C^T[GA][CT]AG
1463 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BanI BbvI
BcnI BmyI BpmI BsaI BsaJI
BsiEI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII