################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI Alw44I AocI ApaI ApaLI AscI Asp718I AspHI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I ClaI CpoI DrdI Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PaeR7I PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI TfiI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccI 229 GTCGAC GT^[AC][GT]AC AcyI 10 GGCGCC G[GA]^CG[CT]C AhdI 1078 GACTATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 155 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 96 AGCGCT AGC^GCT Asp700I 29 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1078 GACTATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1242 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1897 TGGCCA TGG^CCA BbeI 10 GGCGCC GGCGC^C BclI 729 TGATCA T^GATCA BglI 176 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 92 GCTGAGC GC^T[ACGT]AGC Bpu1102I 92 GCTGAGC GC^T[ACGT]AGC BsaHI 10 GGCGCC G[GA]^CG[CT]C BseRI 835 GAGGAG GAGGAG(10/8) BsgI 2009 CTGCAC GTGCAG(16/14) BsmI 483 GAATGC GAATGC(1/-1) Bsp1407I 1360 TGTACA T^GTACA BsrDI 884 GCAATG GCAATG(2/0) BsrFI 1554 GCCGGT [GA]^CCGG[CT] BsrGI 1360 TGTACA T^GTACA BstAPI 1160 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 470 GGTTACC G^GT[ACGT]ACC BstPI 470 GGTTACC G^GT[ACGT]ACC CelII 92 GCTGAGC GC^T[ACGT]AGC Cfr10I 1554 GCCGGT [GA]^CCGG[CT] DraI 2070 TTTAAA TTT^AAA DraIII 2054 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1078 GACTATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 96 AGCGCT AGC^GCT EcoO65I 470 GGTTACC G^GT[ACGT]ACC FbaI 729 TGATCA T^GATCA HgaI 135 GCGTC GACGC(5/10) Hin1I 10 GGCGCC G[GA]^CG[CT]C HincII 229 GTCGAC GT[CT]^[GA]AC HindII 229 GTCGAC GT[CT]^[GA]AC HindIII 798 AAGCTT A^AGCTT KasI 10 GGCGCC G^GCGCC MluNI 1897 TGGCCA TGG^CCA MscI 1897 TGGCCA TGG^CCA MslI 875 CACATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 10 GGCGCC GG^CGCC NdeI 1545 CATATG CA^TATG PacI 1505 TTAATTAA TTAAT^TAA PflFI 1242 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 843 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 749 CTGCAG CTGCA^G PvuII 1655 CAGCTG CAG^CTG SalI 229 GTCGAC G^TCGAC SexAI 1136 ACCAGGT A^CC[AT]GGT SfoI 10 GGCGCC GGC^GCC SmlI 1311 CTCAAG C^T[CT][GA]AG SphI 960 GCATGC GCATG^C SspBI 1360 TGTACA T^GTACA SspI 633 AATATT AAT^ATT StuI 1120 AGGCCT AGG^CCT Tth111I 1242 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 435 TCTAGA T^CTAGA XcmI 644 CCATGGACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 29 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AseI 454 ATTAAT AT^TAAT 1504 ATTAAT AsnI 454 ATTAAT AT^TAAT 1504 ATTAAT AvaI 1331 CCCGAG C^[CT]CG[GA]G 1977 CCCGAG BanII 1648 GGGCTC G[GA]GC[CT]^C 1928 GAGCCC BfaI 368 CTAG C^TAG 436 CTAG BsmFI 1100 GGGAC GGGAC(10/14) 137 GTCCC BsoBI 1331 CCCGAG C^[CT]CG[GA]G 1977 CCCGAG BspHI 405 TCATGA T^CATGA 732 TCATGA DraII 1100 GGGACCT [GA]G^G[ACGT]CC[CT] 1246 GGGTCCT EagI 84 CGGCCG C^GGCCG 116 CGGCCG EclXI 84 CGGCCG C^GGCCG 116 CGGCCG Eco52I 84 CGGCCG C^GGCCG 116 CGGCCG EcoO109I 1100 GGGACCT [GA]G^G[ACGT]CC[CT] 1246 GGGTCCT MaeI 368 CTAG C^TAG 436 CTAG MaeII 239 ACGT A^CGT 1909 ACGT NspI 932 ACATGC [GA]CATG^[CT] 960 GCATGC PpuMI 1100 GGGACCT [GA]G^G[AT]CC[CT] 1246 GGGTCCT PshBI 454 ATTAAT AT^TAAT 1504 ATTAAT RcaI 405 TCATGA T^CATGA 732 TCATGA SapI 976 GAAGAGC GCTCTTC(1/4) 1844 GCTCTTC SfaNI 1190 GCATC GCATC(5/9) 1943 GCATC SfcI 749 CTGCAG C^T[GA][CT]AG 1463 CTGTAG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AvaII BanI BbvI BcnI BmyI BpmI BsaI BsaJI BsiEI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII