##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AlwNI
AocI Aor51HI AscI Asp700I Asp718I
AspI AvaI AviII BalI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BstAPI BstBI BstEII BstPI
BstUI Bsu36I CfoI Cfr10I ClaI
CpoI DsaI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI Sse8387I SwaI TaqI Tth111I
TthHB8I Van91I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AciI 635 CCGC CCGC(-3/-1)
AhdI 83 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1700 GTGCAC G^TGCAC
ApaI 248 GGGCCC GGGCC^C
ApaLI 1700 GTGCAC G^TGCAC
AseI 1361 ATTAAT AT^TAAT
AsnI 1361 ATTAAT AT^TAAT
AspEI 83 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 1081 TGATCA T^GATCA
BcnI 1424 CCGGG CC^[GC]GG
BsaAI 2318 TACGTA [CT]AC^GT[GA]
BsaI 1203 GAGACC GGTCTC(1/5)
BseRI 1380 CTCCTC GAGGAG(10/8)
Bsp1407I 2435 TGTACA T^GTACA
BspMI 2802 GCAGGT ACCTGC(4/8)
BsrGI 2435 TGTACA T^GTACA
BssSI 222 CTCGTG CACGAG(-5/-1)
Bst1107I 2389 GTATAC GTA^TAC
BstXI 2791 CCACTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2389 GTATAC GTA^TAC
DrdI 894 GACAATTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 83 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1081 TGATCA T^GATCA
HapII 1424 CCGG C^CGG
HpaI 2188 GTTAAC GTT^AAC
HpaII 1424 CCGG C^CGG
MspI 1424 CCGG C^CGG
NciI 1424 CCGGG CC^[GC]GG
PshAI 740 GACTTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1361 ATTAAT AT^TAAT
PstI 309 CTGCAG CTGCA^G
SapI 714 GCTCTTC GCTCTTC(1/4)
SnaBI 2318 TACGTA TAC^GTA
SphI 136 GCATGC GCATG^C
SspBI 2435 TGTACA T^GTACA
XbaI 2729 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1646 GTAGAC GT^[AC][GT]AC
2389 GTATAC
BamHI 874 GGATCC G^GATCC
2270 GGATCC
BanI 374 GGCACC G^G[CT][GA]CC
1091 GGTGCC
BbsI 968 GAAGAC GAAGAC(2/6)
1707 GTCTTC
BglII 1912 AGATCT A^GATCT
2845 AGATCT
BlpI 102 GCTCAGC GC^T[ACGT]AGC
532 GCTCAGC
Bpu1102I 102 GCTCAGC GC^T[ACGT]AGC
532 GCTCAGC
BpuAI 968 GAAGAC GAAGAC(2/6)
1707 GTCTTC
BsgI 141 CTGCAC GTGCAG(16/14)
314 GTGCAG
BspHI 2129 TCATGA T^CATGA
2653 TCATGA
BsrDI 217 CATTGC GCAATG(2/0)
1493 GCAATG
BstF5I 331 CATCC GGATG(2/0)
790 GGATG
CelII 102 GCTCAGC GC^T[ACGT]AGC
532 GCTCAGC
DraII 672 GGGACCC [GA]G^G[ACGT]CC[CT]
1689 AGGTCCC
DraIII 950 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
2562 CACATTGTG
EcoO109I 672 GGGACCC [GA]G^G[ACGT]CC[CT]
1689 AGGTCCC
FokI 331 CATCC GGATG(9/13)
790 GGATG
MwoI 722 GCTAGGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1118 GCAGCCAAAGC
NspI 136 GCATGC [GA]CATG^[CT]
1650 ACATGC
PpuMI 672 GGGACCC [GA]G^G[AT]CC[CT]
1689 AGGTCCC
RcaI 2129 TCATGA T^CATGA
2653 TCATGA
SacI 321 GAGCTC GAGCT^C
530 GAGCTC
SspI 2040 AATATT AAT^ATT
2338 AATATT
XcmI 1537 CCAGAAAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1573 CCAGCCTTCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII AvrII BanII BbvI
BfaI BlnI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII