##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AccII AclI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstPI BstUI
BstXI Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV FauI FbaI
FseI FspI HincII HindII HpaI
KasI KspI MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 234 GACGTC GACGT^C
Acc65I 674 GGTACC G^GTACC
AccI 502 GTATAC GT^[AC][GT]AC
AcyI 234 GACGTC G[GA]^CG[CT]C
AflIII 1509 ACATGT A^C[GA][CT]GT
AlwNI 1042 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 407 AGCGCT AGC^GCT
Asp718I 674 GGTACC G^GTACC
AspHI 619 GAGCTC G[AT]GC[AT]^C
AvaI 709 CTCGAG C^[CT]CG[GA]G
AvaII 1020 GGACC G^G[AT]CC
BciVI 582 GGATAC GTATCC(6/5)
BcnI 1778 CCCGG CC^[GC]GG
BglI 1258 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 984 AGATCT A^GATCT
BsaAI 331 TACGTG [CT]AC^GT[GA]
BsaHI 234 GACGTC G[GA]^CG[CT]C
BsaI 1054 GGTCTC GGTCTC(1/5)
BseRI 222 CTCCTC GAGGAG(10/8)
BsiHKAI 619 GAGCTC G[AT]GC[AT]^C
BsmI 817 GAATGC GAATGC(1/-1)
BsoBI 709 CTCGAG C^[CT]CG[GA]G
BspLU11I 1509 ACATGT A^CATGT
BsrDI 1604 GCAATG GCAATG(2/0)
Bst1107I 502 GTATAC GTA^TAC
BstAPI 697 GCACCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 502 GTATAC GTA^TAC
DraI 2106 TTTAAA TTT^AAA
DrdI 234 GACGTCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 956 CCATGG C^C[GA][CT]GG
Eco47III 407 AGCGCT AGC^GCT
EcoT14I 956 CCATGG C^C[AT][AT]GG
EcoT22I 1355 ATGCAT ATGCA^T
HaeII 407 AGCGCT [GA]GCGC^[CT]
Hin1I 234 GACGTC G[GA]^CG[CT]C
KpnI 674 GGTACC GGTAC^C
NciI 1778 CCCGG CC^[GC]GG
NcoI 956 CCATGG C^CATGG
NdeI 1840 CATATG CA^TATG
NheI 1500 GCTAGC G^CTAGC
NsiI 1355 ATGCAT ATGCA^T
PaeR7I 709 CTCGAG C^TCGAG
PvuII 664 CAGCTG CAG^CTG
SacI 619 GAGCTC GAGCT^C
SapI 189 GAAGAGC GCTCTTC(1/4)
SfcI 920 CTGTAG C^T[GA][CT]AG
SspI 1924 AATATT AAT^ATT
StyI 956 CCATGG C^C[AT][AT]GG
Tsp45I 1315 GTCAC ^GT[GC]AC
XhoI 709 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BanI 674 GGTACC G^G[CT][GA]CC
1822 GGTGCC
BanII 192 GAGCCC G[GA]GC[CT]^C
619 GAGCTC
BmyI 192 GAGCCC G[AGT]GC[ACT]^C
619 GAGCTC
BsgI 313 GTGCAG GTGCAG(16/14)
1538 GTGCAG
Bsp1286I 192 GAGCCC G[AGT]GC[ACT]^C
619 GAGCTC
BstYI 414 AGATCC [GA]^GATC[CT]
984 AGATCT
CfoI 408 GCGC GCG^C
594 GCGC
EarI 189 GAAGAG CTCTTC(1/4)
684 GAAGAG
EcoRI 1709 GAATTC G^AATTC
1846 GAATTC
HgaI 549 GACGC GACGC(5/10)
1179 GACGC
HhaI 408 GCGC GCG^C
594 GCGC
HinP1I 408 GCGC G^CGC
594 GCGC
HindIII 778 AAGCTT A^AGCTT
1210 AAGCTT
Ksp632I 189 GAAGAG CTCTTC(1/4)
684 GAAGAG
MflI 414 AGATCC [GA]^GATC[CT]
984 AGATCT
MslI 804 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1987 CACATATATG
MspA1I 664 CAGCTG C[AC]G^C[GT]G
1645 CAGCGG
SphI 1335 GCATGC GCATG^C
1353 GCATGC
StuI 976 AGGCCT AGG^CCT
1542 AGGCCT
XcmI 1037 CCAACCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1411 CCAGAAGAAGTGTGG
XhoII 414 AGATCC [GA]^GATC[CT]
984 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI ApoI BbsI BbvI BfaI
BpmI BpuAI BsaJI BsaWI BseAI
BsiYI BslI BsmAI BsmFI BspEI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MroI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I Van91I