##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AclI AflII AflIII AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbeI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaI BsiEI BsiWI BspDI
BspLU11I BsrBI Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRV FbaI FseI
FspI HaeII HpaI KasI KspI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 169 GGTACC G^GTACC
AccI 391 GTCGAC GT^[AC][GT]AC
AcyI 212 GACGCC G[GA]^CG[CT]C
AlwI 43 GGATC GGATC(4/5)
Asp700I 1967 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 169 GGTACC G^GTACC
BalI 660 TGGCCA TGG^CCA
BbrPI 503 CACGTG CAC^GTG
BsaAI 503 CACGTG [CT]AC^GT[GA]
BsaHI 212 GACGCC G[GA]^CG[CT]C
BsmBI 210 GAGACG CGTCTC(1/5)
BsmI 923 GAATGC GAATGC(1/-1)
Bsp1407I 1158 TGTACA T^GTACA
BspHI 741 TCATGA T^CATGA
BsrDI 1890 CATTGC GCAATG(2/0)
BsrGI 1158 TGTACA T^GTACA
BssSI 2174 CACGAG CACGAG(-5/-1)
BstEII 4 GGTCACC G^GT[ACGT]ACC
BstPI 4 GGTCACC G^GT[ACGT]ACC
DraII 2582 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 2793 CCTTACATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2582 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 4 GGTCACC G^GT[ACGT]ACC
EcoT22I 2922 ATGCAT ATGCA^T
FauI 1269 GCGGG CCCGC(4/6)
Hin1I 212 GACGCC G[GA]^CG[CT]C
HindIII 1809 AAGCTT A^AGCTT
KpnI 169 GGTACC GGTAC^C
MluNI 660 TGGCCA TGG^CCA
MscI 660 TGGCCA TGG^CCA
NsiI 2922 ATGCAT ATGCA^T
NspI 2899 ACATGC [GA]CATG^[CT]
PflMI 2217 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 503 CACGTG CAC^GTG
PmlI 503 CACGTG CAC^GTG
PpuMI 2582 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 741 TCATGA T^CATGA
SacI 737 GAGCTC GAGCT^C
SalI 391 GTCGAC G^TCGAC
SapI 20 GCTCTTC GCTCTTC(1/4)
SexAI 219 ACCTGGT A^CC[AT]GGT
SspBI 1158 TGTACA T^GTACA
Van91I 2217 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1967 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 1075 TCCGGA T^CCGGA
1626 TCCGGA
AspHI 304 GAGCAC G[AT]GC[AT]^C
737 GAGCTC
AspI 791 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1432 GACCATGTC
AvaI 104 CCCGAG C^[CT]CG[GA]G
1902 CTCGGG
BbvCI 544 CCTCAGC CCTCAGC(-5/-2)
1019 GCTGAGG
BcnI 673 CCGGG CC^[GC]GG
1976 CCGGG
BsaBI 2152 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2335 GATATATATC
BseAI 1075 TCCGGA T^CCGGA
1626 TCCGGA
BsiHKAI 304 GAGCAC G[AT]GC[AT]^C
737 GAGCTC
BsoBI 104 CCCGAG C^[CT]CG[GA]G
1902 CTCGGG
BspEI 1075 TCCGGA T^CCGGA
1626 TCCGGA
BssHII 113 GCGCGC G^CGCGC
115 GCGCGC
BstAPI 88 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1480 GCAGGCTGTGC
DrdI 197 GACTTTTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1697 GACACTTTTGTC
EaeI 64 CGGCCA [CT]^GGCC[GA]
660 TGGCCA
EarI 21 CTCTTC CTCTTC(1/4)
2133 GAAGAG
EcoRI 1818 GAATTC G^AATTC
1844 GAATTC
HgaI 212 GACGC GACGC(5/10)
640 GCGTC
HincII 391 GTCGAC GT[CT]^[GA]AC
1664 GTTGAC
HindII 391 GTCGAC GT[CT]^[GA]AC
1664 GTTGAC
Ksp632I 21 CTCTTC CTCTTC(1/4)
2133 GAAGAG
MamI 2152 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2335 GATATATATC
MroI 1075 TCCGGA T^CCGGA
1626 TCCGGA
NciI 673 CCGGG CC^[GC]GG
1976 CCGGG
NheI 1754 GCTAGC G^CTAGC
2619 GCTAGC
PflFI 791 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1432 GACCATGTC
PstI 95 CTGCAG CTGCA^G
137 CTGCAG
SfcI 95 CTGCAG C^T[GA][CT]AG
137 CTGCAG
Tth111I 791 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1432 GACCATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AgeI
AluI AlwNI ApoI AvaII BanI
BanII BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV PinAI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII