################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AclI AflII AflIII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AviII AvrII BamHI BbeI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaI BsiEI BsiWI BspDI BspLU11I BsrBI Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV FbaI FseI FspI HaeII HpaI KasI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 169 GGTACC G^GTACC AccI 391 GTCGAC GT^[AC][GT]AC AcyI 212 GACGCC G[GA]^CG[CT]C AlwI 43 GGATC GGATC(4/5) Asp700I 1967 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 169 GGTACC G^GTACC BalI 660 TGGCCA TGG^CCA BbrPI 503 CACGTG CAC^GTG BsaAI 503 CACGTG [CT]AC^GT[GA] BsaHI 212 GACGCC G[GA]^CG[CT]C BsmBI 210 GAGACG CGTCTC(1/5) BsmI 923 GAATGC GAATGC(1/-1) Bsp1407I 1158 TGTACA T^GTACA BspHI 741 TCATGA T^CATGA BsrDI 1890 CATTGC GCAATG(2/0) BsrGI 1158 TGTACA T^GTACA BssSI 2174 CACGAG CACGAG(-5/-1) BstEII 4 GGTCACC G^GT[ACGT]ACC BstPI 4 GGTCACC G^GT[ACGT]ACC DraII 2582 GGGACCT [GA]G^G[ACGT]CC[CT] EcoNI 2793 CCTTACATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 2582 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 4 GGTCACC G^GT[ACGT]ACC EcoT22I 2922 ATGCAT ATGCA^T FauI 1269 GCGGG CCCGC(4/6) Hin1I 212 GACGCC G[GA]^CG[CT]C HindIII 1809 AAGCTT A^AGCTT KpnI 169 GGTACC GGTAC^C MluNI 660 TGGCCA TGG^CCA MscI 660 TGGCCA TGG^CCA NsiI 2922 ATGCAT ATGCA^T NspI 2899 ACATGC [GA]CATG^[CT] PflMI 2217 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 503 CACGTG CAC^GTG PmlI 503 CACGTG CAC^GTG PpuMI 2582 GGGACCT [GA]G^G[AT]CC[CT] RcaI 741 TCATGA T^CATGA SacI 737 GAGCTC GAGCT^C SalI 391 GTCGAC G^TCGAC SapI 20 GCTCTTC GCTCTTC(1/4) SexAI 219 ACCTGGT A^CC[AT]GGT SspBI 1158 TGTACA T^GTACA Van91I 2217 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1967 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccIII 1075 TCCGGA T^CCGGA 1626 TCCGGA AspHI 304 GAGCAC G[AT]GC[AT]^C 737 GAGCTC AspI 791 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1432 GACCATGTC AvaI 104 CCCGAG C^[CT]CG[GA]G 1902 CTCGGG BbvCI 544 CCTCAGC CCTCAGC(-5/-2) 1019 GCTGAGG BcnI 673 CCGGG CC^[GC]GG 1976 CCGGG BsaBI 2152 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2335 GATATATATC BseAI 1075 TCCGGA T^CCGGA 1626 TCCGGA BsiHKAI 304 GAGCAC G[AT]GC[AT]^C 737 GAGCTC BsoBI 104 CCCGAG C^[CT]CG[GA]G 1902 CTCGGG BspEI 1075 TCCGGA T^CCGGA 1626 TCCGGA BssHII 113 GCGCGC G^CGCGC 115 GCGCGC BstAPI 88 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1480 GCAGGCTGTGC DrdI 197 GACTTTTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1697 GACACTTTTGTC EaeI 64 CGGCCA [CT]^GGCC[GA] 660 TGGCCA EarI 21 CTCTTC CTCTTC(1/4) 2133 GAAGAG EcoRI 1818 GAATTC G^AATTC 1844 GAATTC HgaI 212 GACGC GACGC(5/10) 640 GCGTC HincII 391 GTCGAC GT[CT]^[GA]AC 1664 GTTGAC HindII 391 GTCGAC GT[CT]^[GA]AC 1664 GTTGAC Ksp632I 21 CTCTTC CTCTTC(1/4) 2133 GAAGAG MamI 2152 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2335 GATATATATC MroI 1075 TCCGGA T^CCGGA 1626 TCCGGA NciI 673 CCGGG CC^[GC]GG 1976 CCGGG NheI 1754 GCTAGC G^CTAGC 2619 GCTAGC PflFI 791 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1432 GACCATGTC PstI 95 CTGCAG CTGCA^G 137 CTGCAG SfcI 95 CTGCAG C^T[GA][CT]AG 137 CTGCAG Tth111I 791 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1432 GACCATGTC ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcsI AfaI AgeI AluI AlwNI ApoI AvaII BanI BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV PinAI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII