##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AhdI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbrPI
BciVI BfrI BlnI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
KpnI KspI MluI MroI NaeI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfuI SpeI SphI
Sse8387I SspI SwaI TfiI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 2585 GACGTC GACGT^C
AccI 2819 GTCTAC GT^[AC][GT]AC
AgeI 168 ACCGGT A^CCGGT
Alw44I 2828 GTGCAC G^TGCAC
AocI 1166 CCTGAGG CC^T[ACGT]AGG
ApaLI 2828 GTGCAC G^TGCAC
AviII 432 TGCGCA TGC^GCA
BalI 1180 TGGCCA TGG^CCA
BbeI 41 GGCGCC GGCGC^C
BcgI 789 GCACCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1834 TGATCA T^GATCA
BlpI 1859 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1859 GCTGAGC GC^T[ACGT]AGC
BsaAI 2662 TACGTA [CT]AC^GT[GA]
BsaBI 277 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 642 GAGACC GGTCTC(1/5)
BsaWI 168 ACCGGT [AT]^CCGG[AT]
Bsp1407I 348 TGTACA T^GTACA
BsrBI 86 GAGCGG CCGCTC(-3/-3)
BsrDI 2647 CATTGC GCAATG(2/0)
BsrGI 348 TGTACA T^GTACA
BssHII 55 GCGCGC G^CGCGC
BstXI 2732 CCAGCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1166 CCTGAGG CC^T[ACGT]AGG
CelII 1859 GCTGAGC GC^T[ACGT]AGC
DraI 2285 TTTAAA TTT^AAA
Eco81I 1166 CCTGAGG CC^T[ACGT]AGG
FbaI 1834 TGATCA T^GATCA
FspI 432 TGCGCA TGC^GCA
HgaI 1189 GCGTC GACGC(5/10)
KasI 41 GGCGCC G^GCGCC
MamI 277 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3056 CAATTG C^AATTG
MluNI 1180 TGGCCA TGG^CCA
MscI 1180 TGGCCA TGG^CCA
MslI 659 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3056 CAATTG C^AATTG
NarI 41 GGCGCC GG^CGCC
NcoI 24 CCATGG C^CATGG
NheI 2170 GCTAGC G^CTAGC
NspI 429 ACATGC [GA]CATG^[CT]
PinAI 168 ACCGGT A^CCGGT
SapI 448 GCTCTTC GCTCTTC(1/4)
SfoI 41 GGCGCC GGC^GCC
SgrAI 1184 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 2662 TACGTA TAC^GTA
SspBI 348 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 2206 GAATTT [GA]^AATT[CT]
3201 AAATTT
AlwI 277 GATCC GGATC(4/5)
2655 GGATC
Aor51HI 204 AGCGCT AGC^GCT
600 AGCGCT
ApaI 678 GGGCCC GGGCC^C
2752 GGGCCC
ApoI 2206 GAATTT [GA]^AATT[CT]
3201 AAATTT
BbvCI 1233 CCTCAGC CCTCAGC(-5/-2)
2863 GCTGAGG
BssSI 299 CACGAG CACGAG(-5/-1)
2973 CTCGTG
DraIII 155 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2577 CACACGGTG
Eco47III 204 AGCGCT AGC^GCT
600 AGCGCT
EcoNI 739 CCTGTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1979 CCTCCCCCAGG
MaeII 2586 ACGT A^CGT
2663 ACGT
SacI 446 GAGCTC GAGCT^C
1328 GAGCTC
ScaI 2334 AGTACT AGT^ACT
3214 AGTACT
SfiI 965 GGCCGAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2949 GGCCTCAGTGGCC
TaqI 529 TCGA T^CGA
798 TCGA
TthHB8I 529 TCGA T^CGA
798 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII
XmaCI XmaI XmnI