################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbeI BbrPI BciVI BclI BfrI BlnI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI BstEII BstPI BstXI Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NarI NdeI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 119 GACGTC GACGT^C AccI 1393 GTATAC GT^[AC][GT]AC AspI 1957 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2007 GGATCC G^GATCC BanI 82 GGTGCC G^G[CT][GA]CC BsaAI 463 CACGTA [CT]AC^GT[GA] BsaBI 139 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1490 GGTCTC GGTCTC(1/5) BsaWI 1561 ACCGGA [AT]^CCGG[AT] BsgI 1328 GTGCAG GTGCAG(16/14) BssHII 289 GCGCGC G^CGCGC Bst1107I 1393 GTATAC GTA^TAC BstBI 1541 TTCGAA TT^CGAA BstZ17I 1393 GTATAC GTA^TAC DraIII 1825 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG MamI 139 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 2133 TTAA T^TAA MslI 448 CATCGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1710 CCATGG C^CATGG NheI 2103 GCTAGC G^CTAGC NspV 1541 TTCGAA TT^CGAA PflFI 1957 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1824 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 806 AGGACCT [GA]G^G[AT]CC[CT] PvuII 1303 CAGCTG CAG^CTG ScaI 1894 AGTACT AGT^ACT SfuI 1541 TTCGAA TT^CGAA SmaI 107 CCCGGG CCC^GGG SspI 295 AATATT AAT^ATT Tru9I 2133 TTAA T^TAA Tth111I 1957 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1824 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1018 TCTAGA T^CTAGA XmaCI 107 CCCGGG C^CCGGG XmaI 107 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 963 GAATTC [GA]^AATT[CT] 2094 GAATTC ApoI 963 GAATTC [GA]^AATT[CT] 2094 GAATTC AspHI 1014 GAGCTC G[AT]GC[AT]^C 1923 GAGCTC BcgI 486 GCAGCTACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1650 GCAGCGTCGTCG BglII 857 AGATCT A^GATCT 2125 AGATCT BlpI 1111 GCTGAGC GC^T[ACGT]AGC 1197 GCTGAGC Bpu1102I 1111 GCTGAGC GC^T[ACGT]AGC 1197 GCTGAGC BsiEI 87 CGGCCG CG[GA][CT]^CG 132 CGGCCG BsiHKAI 1014 GAGCTC G[AT]GC[AT]^C 1923 GAGCTC BspMI 666 GCAGGT ACCTGC(4/8) 1032 GCAGGT BsrBI 53 CCGCTC CCGCTC(-3/-3) 413 CCGCTC BsrDI 834 CATTGC GCAATG(2/0) 1122 CATTGC BstAPI 1032 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1140 GCAGCGCTTGC CelII 1111 GCTGAGC GC^T[ACGT]AGC 1197 GCTGAGC DraII 179 AGGCCCC [GA]G^G[ACGT]CC[CT] 806 AGGACCT DsaI 711 CCGTGG C^C[GA][CT]GG 1710 CCATGG EagI 87 CGGCCG C^GGCCG 132 CGGCCG EarI 538 GAAGAG CTCTTC(1/4) 1863 GAAGAG EclXI 87 CGGCCG C^GGCCG 132 CGGCCG Eco52I 87 CGGCCG C^GGCCG 132 CGGCCG EcoNI 1071 CCTCTATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1265 CCTGCCGCAGG EcoO109I 179 AGGCCCC [GA]G^G[ACGT]CC[CT] 806 AGGACCT EcoRI 963 GAATTC G^AATTC 2094 GAATTC Ksp632I 538 GAAGAG CTCTTC(1/4) 1863 GAAGAG MaeII 120 ACGT A^CGT 464 ACGT NspI 1981 ACATGC [GA]CATG^[CT] 2041 ACATGC SacI 1014 GAGCTC GAGCT^C 1923 GAGCTC TfiI 795 GAATC G^A[AT]TC 1806 GATTC Tsp45I 1477 GTGAC ^GT[GC]AC 1639 GTGAC TspRI 916 CACTG CA[GC]TG[ACGT][ACGT]^ 1637 CAGTG XcmI 23 CCAAAGCACGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1587 CCAAAGCCCCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI Aor51HI AvaI AvaII BalI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI Eco47III EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp509I TthHB8I XhoII