##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbeI
BbrPI BciVI BclI BfrI BlnI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssSI BstEII BstPI BstXI Bsu36I
ClaI CpoI DraI DrdI Eam1105I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NarI NdeI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 119 GACGTC GACGT^C
AccI 1393 GTATAC GT^[AC][GT]AC
AspI 1957 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2007 GGATCC G^GATCC
BanI 82 GGTGCC G^G[CT][GA]CC
BsaAI 463 CACGTA [CT]AC^GT[GA]
BsaBI 139 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1490 GGTCTC GGTCTC(1/5)
BsaWI 1561 ACCGGA [AT]^CCGG[AT]
BsgI 1328 GTGCAG GTGCAG(16/14)
BssHII 289 GCGCGC G^CGCGC
Bst1107I 1393 GTATAC GTA^TAC
BstBI 1541 TTCGAA TT^CGAA
BstZ17I 1393 GTATAC GTA^TAC
DraIII 1825 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
MamI 139 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2133 TTAA T^TAA
MslI 448 CATCGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1710 CCATGG C^CATGG
NheI 2103 GCTAGC G^CTAGC
NspV 1541 TTCGAA TT^CGAA
PflFI 1957 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1824 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 806 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1303 CAGCTG CAG^CTG
ScaI 1894 AGTACT AGT^ACT
SfuI 1541 TTCGAA TT^CGAA
SmaI 107 CCCGGG CCC^GGG
SspI 295 AATATT AAT^ATT
Tru9I 2133 TTAA T^TAA
Tth111I 1957 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1824 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1018 TCTAGA T^CTAGA
XmaCI 107 CCCGGG C^CCGGG
XmaI 107 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 963 GAATTC [GA]^AATT[CT]
2094 GAATTC
ApoI 963 GAATTC [GA]^AATT[CT]
2094 GAATTC
AspHI 1014 GAGCTC G[AT]GC[AT]^C
1923 GAGCTC
BcgI 486 GCAGCTACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1650 GCAGCGTCGTCG
BglII 857 AGATCT A^GATCT
2125 AGATCT
BlpI 1111 GCTGAGC GC^T[ACGT]AGC
1197 GCTGAGC
Bpu1102I 1111 GCTGAGC GC^T[ACGT]AGC
1197 GCTGAGC
BsiEI 87 CGGCCG CG[GA][CT]^CG
132 CGGCCG
BsiHKAI 1014 GAGCTC G[AT]GC[AT]^C
1923 GAGCTC
BspMI 666 GCAGGT ACCTGC(4/8)
1032 GCAGGT
BsrBI 53 CCGCTC CCGCTC(-3/-3)
413 CCGCTC
BsrDI 834 CATTGC GCAATG(2/0)
1122 CATTGC
BstAPI 1032 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1140 GCAGCGCTTGC
CelII 1111 GCTGAGC GC^T[ACGT]AGC
1197 GCTGAGC
DraII 179 AGGCCCC [GA]G^G[ACGT]CC[CT]
806 AGGACCT
DsaI 711 CCGTGG C^C[GA][CT]GG
1710 CCATGG
EagI 87 CGGCCG C^GGCCG
132 CGGCCG
EarI 538 GAAGAG CTCTTC(1/4)
1863 GAAGAG
EclXI 87 CGGCCG C^GGCCG
132 CGGCCG
Eco52I 87 CGGCCG C^GGCCG
132 CGGCCG
EcoNI 1071 CCTCTATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1265 CCTGCCGCAGG
EcoO109I 179 AGGCCCC [GA]G^G[ACGT]CC[CT]
806 AGGACCT
EcoRI 963 GAATTC G^AATTC
2094 GAATTC
Ksp632I 538 GAAGAG CTCTTC(1/4)
1863 GAAGAG
MaeII 120 ACGT A^CGT
464 ACGT
NspI 1981 ACATGC [GA]CATG^[CT]
2041 ACATGC
SacI 1014 GAGCTC GAGCT^C
1923 GAGCTC
TfiI 795 GAATC G^A[AT]TC
1806 GATTC
Tsp45I 1477 GTGAC ^GT[GC]AC
1639 GTGAC
TspRI 916 CACTG CA[GC]TG[ACGT][ACGT]^
1637 CAGTG
XcmI 23 CCAAAGCACGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1587 CCAAAGCCCCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI AvaI AvaII
BalI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII EaeI Eco47III
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp509I TthHB8I XhoII