################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 1329 GGTACC G^GTACC AclI 1457 AACGTT AA^CGTT AcsI 447 GAATTC [GA]^AATT[CT] AocI 843 CCTAAGG CC^T[ACGT]AGG ApoI 447 GAATTC [GA]^AATT[CT] Asp700I 443 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1329 GGTACC G^GTACC AspHI 782 GAGCTC G[AT]GC[AT]^C BalI 152 TGGCCA TGG^CCA BamHI 122 GGATCC G^GATCC BbsI 2107 GAAGAC GAAGAC(2/6) BfaI 550 CTAG C^TAG BglII 535 AGATCT A^GATCT BpuAI 2107 GAAGAC GAAGAC(2/6) BsaWI 271 ACCGGA [AT]^CCGG[AT] BsiHKAI 782 GAGCTC G[AT]GC[AT]^C BspMI 191 GCAGGT ACCTGC(4/8) BsrDI 931 GCAATG GCAATG(2/0) BsrFI 2038 ACCGGC [GA]^CCGG[CT] BssSI 1899 CACGAG CACGAG(-5/-1) BstEII 1091 GGTGACC G^GT[ACGT]ACC BstPI 1091 GGTGACC G^GT[ACGT]ACC Bsu36I 843 CCTAAGG CC^T[ACGT]AGG Cfr10I 2038 ACCGGC [GA]^CCGG[CT] DraIII 1561 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 152 TGGCCA [CT]^GGCC[GA] Eco81I 843 CCTAAGG CC^T[ACGT]AGG EcoNI 1592 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1091 GGTGACC G^GT[ACGT]ACC EcoRI 447 GAATTC G^AATTC KpnI 1329 GGTACC GGTAC^C MaeI 550 CTAG C^TAG MluNI 152 TGGCCA TGG^CCA MscI 152 TGGCCA TGG^CCA PaeR7I 471 CTCGAG C^TCGAG PflMI 896 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 1457 AACGTT AA^CGTT SacI 782 GAGCTC GAGCT^C SapI 1784 GCTCTTC GCTCTTC(1/4) SphI 2068 GCATGC GCATG^C SspI 2282 AATATT AAT^ATT Tsp509I 448 AATT ^AATT Van91I 896 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 471 CTCGAG C^TCGAG XmnI 443 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### BcnI 1690 CCCGG CC^[GC]GG 2021 CCGGG BsaI 69 GAGACC GGTCTC(1/5) 330 GAGACC BseRI 2227 CTCCTC GAGGAG(10/8) 1902 GAGGAG BsgI 608 CTGCAC GTGCAG(16/14) 883 CTGCAC BsmBI 33 GAGACG CGTCTC(1/5) 1779 GAGACG BsmI 343 GAATGC GAATGC(1/-1) 1672 GCATTC BstYI 122 GGATCC [GA]^GATC[CT] 535 AGATCT FauI 922 GCGGG CCCGC(4/6) 967 GCGGG HaeII 488 GGCGCT [GA]GCGC^[CT] 1765 AGCGCC HgaI 35 GACGC GACGC(5/10) 1781 GACGC HincII 411 GTTGAC GT[CT]^[GA]AC 1772 GTCAAC HindII 411 GTTGAC GT[CT]^[GA]AC 1772 GTCAAC MaeII 409 ACGT A^CGT 1458 ACGT MflI 122 GGATCC [GA]^GATC[CT] 535 AGATCT MseI 183 TTAA T^TAA 1339 TTAA NciI 1690 CCCGG CC^[GC]GG 2021 CCGGG NspI 1645 ACATGC [GA]CATG^[CT] 2068 GCATGC PstI 1084 CTGCAG CTGCA^G 1530 CTGCAG ScaI 403 AGTACT AGT^ACT 1825 AGTACT SfaNI 372 GATGC GCATC(5/9) 1727 GATGC SmlI 471 CTCGAG C^T[CT][GA]AG 779 CTTGAG TfiI 1185 GAATC G^A[AT]TC 1864 GATTC Tru9I 183 TTAA T^TAA 1339 TTAA XhoII 122 GGATCC [GA]^GATC[CT] 535 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AvaI AvaII BanI BanII BbvI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I