##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BseAI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      Bst1107I    
BstBI       BstUI       BstZ17I     CelII       ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRV       EcoT22I     
FbaI        FseI        FspI        Hin1I       HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MroI        MslI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        RcaI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I       1329  GGTACC               G^GTACC
AclI         1457  AACGTT               AA^CGTT
AcsI          447  GAATTC               [GA]^AATT[CT]
AocI          843  CCTAAGG              CC^T[ACGT]AGG
ApoI          447  GAATTC               [GA]^AATT[CT]
Asp700I       443  GAACGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1329  GGTACC               G^GTACC
AspHI         782  GAGCTC               G[AT]GC[AT]^C
BalI          152  TGGCCA               TGG^CCA
BamHI         122  GGATCC               G^GATCC
BbsI         2107  GAAGAC               GAAGAC(2/6)
BfaI          550  CTAG                 C^TAG
BglII         535  AGATCT               A^GATCT
BpuAI        2107  GAAGAC               GAAGAC(2/6)
BsaWI         271  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       782  GAGCTC               G[AT]GC[AT]^C
BspMI         191  GCAGGT               ACCTGC(4/8)
BsrDI         931  GCAATG               GCAATG(2/0)
BsrFI        2038  ACCGGC               [GA]^CCGG[CT]
BssSI        1899  CACGAG               CACGAG(-5/-1)
BstEII       1091  GGTGACC              G^GT[ACGT]ACC
BstPI        1091  GGTGACC              G^GT[ACGT]ACC
Bsu36I        843  CCTAAGG              CC^T[ACGT]AGG
Cfr10I       2038  ACCGGC               [GA]^CCGG[CT]
DraIII       1561  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          152  TGGCCA               [CT]^GGCC[GA]
Eco81I        843  CCTAAGG              CC^T[ACGT]AGG
EcoNI        1592  CCTCACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1091  GGTGACC              G^GT[ACGT]ACC
EcoRI         447  GAATTC               G^AATTC
KpnI         1329  GGTACC               GGTAC^C
MaeI          550  CTAG                 C^TAG
MluNI         152  TGGCCA               TGG^CCA
MscI          152  TGGCCA               TGG^CCA
PaeR7I        471  CTCGAG               C^TCGAG
PflMI         896  CCAGGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I     1457  AACGTT               AA^CGTT
SacI          782  GAGCTC               GAGCT^C
SapI         1784  GCTCTTC              GCTCTTC(1/4)
SphI         2068  GCATGC               GCATG^C
SspI         2282  AATATT               AAT^ATT
Tsp509I       448  AATT                 ^AATT
Van91I        896  CCAGGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          471  CTCGAG               C^TCGAG
XmnI          443  GAACGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

BcnI         1690  CCCGG                CC^[GC]GG
             2021  CCGGG               
BsaI           69  GAGACC               GGTCTC(1/5)
              330  GAGACC              
BseRI        2227  CTCCTC               GAGGAG(10/8)
             1902  GAGGAG              
BsgI          608  CTGCAC               GTGCAG(16/14)
              883  CTGCAC              
BsmBI          33  GAGACG               CGTCTC(1/5)
             1779  GAGACG              
BsmI          343  GAATGC               GAATGC(1/-1)
             1672  GCATTC              
BstYI         122  GGATCC               [GA]^GATC[CT]
              535  AGATCT              
FauI          922  GCGGG                CCCGC(4/6)
              967  GCGGG               
HaeII         488  GGCGCT               [GA]GCGC^[CT]
             1765  AGCGCC              
HgaI           35  GACGC                GACGC(5/10)
             1781  GACGC               
HincII        411  GTTGAC               GT[CT]^[GA]AC
             1772  GTCAAC              
HindII        411  GTTGAC               GT[CT]^[GA]AC
             1772  GTCAAC              
MaeII         409  ACGT                 A^CGT
             1458  ACGT                
MflI          122  GGATCC               [GA]^GATC[CT]
              535  AGATCT              
MseI          183  TTAA                 T^TAA
             1339  TTAA                
NciI         1690  CCCGG                CC^[GC]GG
             2021  CCGGG               
NspI         1645  ACATGC               [GA]CATG^[CT]
             2068  GCATGC              
PstI         1084  CTGCAG               CTGCA^G
             1530  CTGCAG              
ScaI          403  AGTACT               AGT^ACT
             1825  AGTACT              
SfaNI         372  GATGC                GCATC(5/9)
             1727  GATGC               
SmlI          471  CTCGAG               C^T[CT][GA]AG
              779  CTTGAG              
TfiI         1185  GAATC                G^A[AT]TC
             1864  GATTC               
Tru9I         183  TTAA                 T^TAA
             1339  TTAA                
XhoII         122  GGATCC               [GA]^GATC[CT]
              535  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
ApaI        AvaI        AvaII       BanI        BanII       
BbvI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NcoI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I