##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccI AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MslI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1329 GGTACC G^GTACC
AclI 1457 AACGTT AA^CGTT
AcsI 447 GAATTC [GA]^AATT[CT]
AocI 843 CCTAAGG CC^T[ACGT]AGG
ApoI 447 GAATTC [GA]^AATT[CT]
Asp700I 443 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1329 GGTACC G^GTACC
AspHI 782 GAGCTC G[AT]GC[AT]^C
BalI 152 TGGCCA TGG^CCA
BamHI 122 GGATCC G^GATCC
BbsI 2107 GAAGAC GAAGAC(2/6)
BfaI 550 CTAG C^TAG
BglII 535 AGATCT A^GATCT
BpuAI 2107 GAAGAC GAAGAC(2/6)
BsaWI 271 ACCGGA [AT]^CCGG[AT]
BsiHKAI 782 GAGCTC G[AT]GC[AT]^C
BspMI 191 GCAGGT ACCTGC(4/8)
BsrDI 931 GCAATG GCAATG(2/0)
BsrFI 2038 ACCGGC [GA]^CCGG[CT]
BssSI 1899 CACGAG CACGAG(-5/-1)
BstEII 1091 GGTGACC G^GT[ACGT]ACC
BstPI 1091 GGTGACC G^GT[ACGT]ACC
Bsu36I 843 CCTAAGG CC^T[ACGT]AGG
Cfr10I 2038 ACCGGC [GA]^CCGG[CT]
DraIII 1561 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 152 TGGCCA [CT]^GGCC[GA]
Eco81I 843 CCTAAGG CC^T[ACGT]AGG
EcoNI 1592 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1091 GGTGACC G^GT[ACGT]ACC
EcoRI 447 GAATTC G^AATTC
KpnI 1329 GGTACC GGTAC^C
MaeI 550 CTAG C^TAG
MluNI 152 TGGCCA TGG^CCA
MscI 152 TGGCCA TGG^CCA
PaeR7I 471 CTCGAG C^TCGAG
PflMI 896 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1457 AACGTT AA^CGTT
SacI 782 GAGCTC GAGCT^C
SapI 1784 GCTCTTC GCTCTTC(1/4)
SphI 2068 GCATGC GCATG^C
SspI 2282 AATATT AAT^ATT
Tsp509I 448 AATT ^AATT
Van91I 896 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 471 CTCGAG C^TCGAG
XmnI 443 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BcnI 1690 CCCGG CC^[GC]GG
2021 CCGGG
BsaI 69 GAGACC GGTCTC(1/5)
330 GAGACC
BseRI 2227 CTCCTC GAGGAG(10/8)
1902 GAGGAG
BsgI 608 CTGCAC GTGCAG(16/14)
883 CTGCAC
BsmBI 33 GAGACG CGTCTC(1/5)
1779 GAGACG
BsmI 343 GAATGC GAATGC(1/-1)
1672 GCATTC
BstYI 122 GGATCC [GA]^GATC[CT]
535 AGATCT
FauI 922 GCGGG CCCGC(4/6)
967 GCGGG
HaeII 488 GGCGCT [GA]GCGC^[CT]
1765 AGCGCC
HgaI 35 GACGC GACGC(5/10)
1781 GACGC
HincII 411 GTTGAC GT[CT]^[GA]AC
1772 GTCAAC
HindII 411 GTTGAC GT[CT]^[GA]AC
1772 GTCAAC
MaeII 409 ACGT A^CGT
1458 ACGT
MflI 122 GGATCC [GA]^GATC[CT]
535 AGATCT
MseI 183 TTAA T^TAA
1339 TTAA
NciI 1690 CCCGG CC^[GC]GG
2021 CCGGG
NspI 1645 ACATGC [GA]CATG^[CT]
2068 GCATGC
PstI 1084 CTGCAG CTGCA^G
1530 CTGCAG
ScaI 403 AGTACT AGT^ACT
1825 AGTACT
SfaNI 372 GATGC GCATC(5/9)
1727 GATGC
SmlI 471 CTCGAG C^T[CT][GA]AG
779 CTTGAG
TfiI 1185 GAATC G^A[AT]TC
1864 GATTC
Tru9I 183 TTAA T^TAA
1339 TTAA
XhoII 122 GGATCC [GA]^GATC[CT]
535 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaI AvaII BanI BanII
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I