################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## Acc65I AflIII AgeI AhdI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanII BbvCI BcgI BcnI BlnI BsaBI BsiEI BsiWI BspDI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FseI FspI HindIII HpaI KpnI KspI MamI MluI MluNI MscI NaeI NciI NgoMIV NotI NruI PacI PflFI PinAI PmeI PpuMI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 79 GACGTC GACGT^C AccI 1178 GTCTAC GT^[AC][GT]AC AccII 2555 CGCG CG^CG AclI 367 AACGTT AA^CGTT AflII 2630 CTTAAG C^TTAAG Alw44I 1770 GTGCAC G^TGCAC ApaLI 1770 GTGCAC G^TGCAC AseI 3252 ATTAAT AT^TAAT AsnI 3252 ATTAAT AT^TAAT AvaI 1822 CTCGAG C^[CT]CG[GA]G BbeI 2695 GGCGCC GGCGC^C BbrPI 1478 CACGTG CAC^GTG BciVI 2958 GTATCC GTATCC(6/5) BfrI 2630 CTTAAG C^TTAAG BglII 1818 AGATCT A^GATCT BsaAI 1478 CACGTG [CT]AC^GT[GA] BseRI 2042 GAGGAG GAGGAG(10/8) BsoBI 1822 CTCGAG C^[CT]CG[GA]G Bsp1407I 939 TGTACA T^GTACA BspHI 1758 TCATGA T^CATGA BsrDI 1890 CATTGC GCAATG(2/0) BsrGI 939 TGTACA T^GTACA BstAPI 552 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 371 TTCGAA TT^CGAA BstUI 2555 CGCG CG^CG DraI 2850 TTTAAA TTT^AAA DsaI 128 CCATGG C^C[GA][CT]GG HaeII 2695 GGCGCC [GA]GCGC^[CT] HgaI 1593 GCGTC GACGC(5/10) KasI 2695 GGCGCC G^GCGCC MvnI 2555 CGCG CG^CG NarI 2695 GGCGCC GG^CGCC NcoI 128 CCATGG C^CATGG NheI 2076 GCTAGC G^CTAGC NspI 1279 ACATGC [GA]CATG^[CT] NspV 371 TTCGAA TT^CGAA PaeR7I 1822 CTCGAG C^TCGAG PmaCI 1478 CACGTG CAC^GTG PmlI 1478 CACGTG CAC^GTG PshAI 1303 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3252 ATTAAT AT^TAAT Psp1406I 367 AACGTT AA^CGTT RcaI 1758 TCATGA T^CATGA ScaI 2375 AGTACT AGT^ACT SexAI 1025 ACCTGGT A^CC[AT]GGT SfoI 2695 GGCGCC GGC^GCC SfuI 371 TTCGAA TT^CGAA SspBI 939 TGTACA T^GTACA SspI 1832 AATATT AAT^ATT StuI 1331 AGGCCT AGG^CCT XbaI 909 TCTAGA T^CTAGA XcmI 1684 CCAGAGTATGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1822 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccIII 104 TCCGGA T^CCGGA 1347 TCCGGA AcyI 79 GACGTC G[GA]^CG[CT]C 2695 GGCGCC AspHI 22 GAGCAC G[AT]GC[AT]^C 1770 GTGCAC BclI 212 TGATCA T^GATCA 3288 TGATCA BmyI 22 GAGCAC G[AGT]GC[ACT]^C 1770 GTGCAC BsaHI 79 GACGTC G[GA]^CG[CT]C 2695 GGCGCC BsaI 470 GAGACC GGTCTC(1/5) 2334 GGTCTC BsaWI 104 TCCGGA [AT]^CCGG[AT] 1347 TCCGGA BseAI 104 TCCGGA T^CCGGA 1347 TCCGGA BsgI 2713 CTGCAC GTGCAG(16/14) 1042 GTGCAG BsiHKAI 22 GAGCAC G[AT]GC[AT]^C 1770 GTGCAC BsmBI 2793 CGTCTC CGTCTC(1/5) 43 GAGACG BsmI 1847 GCATTC GAATGC(1/-1) 2708 GCATTC Bsp1286I 22 GAGCAC G[AGT]GC[ACT]^C 1770 GTGCAC BspEI 104 TCCGGA T^CCGGA 1347 TCCGGA BspMI 557 ACCTGC ACCTGC(4/8) 1243 GCAGGT BstYI 1419 GGATCT [GA]^GATC[CT] 1818 AGATCT CfoI 1922 GCGC GCG^C 2696 GCGC FbaI 212 TGATCA T^GATCA 3288 TGATCA HapII 105 CCGG C^CGG 1348 CCGG HhaI 1922 GCGC GCG^C 2696 GCGC Hin1I 79 GACGTC G[GA]^CG[CT]C 2695 GGCGCC HinP1I 1922 GCGC G^CGC 2696 GCGC HpaII 105 CCGG C^CGG 1348 CCGG MfeI 943 CAATTG C^AATTG 1662 CAATTG MflI 1419 GGATCT [GA]^GATC[CT] 1818 AGATCT MroI 104 TCCGGA T^CCGGA 1347 TCCGGA MslI 129 CATGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2194 CATCATTGTG MspI 105 CCGG C^CGG 1348 CCGG MunI 943 CAATTG C^AATTG 1662 CAATTG NdeI 1325 CATATG CA^TATG 3095 CATATG PflMI 1708 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2454 CCAGGCTCTGG PstI 851 CTGCAG CTGCA^G 1414 CTGCAG Tsp45I 2483 GTCAC ^GT[GC]AC 2950 GTCAC Van91I 1708 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2454 CCAGGCTCTGG XhoII 1419 GGATCT [GA]^GATC[CT] 1818 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BanI BbsI BbvI BfaI BglI BlpI BpmI Bpu1102I BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI BstXI Cac8I CelII Cfr13I DdeI DpnII EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV NsiI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I