##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AflIII AgeI AhdI AocI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanII BbvCI BcgI BcnI
BlnI BsaBI BsiEI BsiWI BspDI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FseI
FspI HindIII HpaI KpnI KspI
MamI MluI MluNI MscI NaeI
NciI NgoMIV NotI NruI PacI
PflFI PinAI PmeI PpuMI PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 79 GACGTC GACGT^C
AccI 1178 GTCTAC GT^[AC][GT]AC
AccII 2555 CGCG CG^CG
AclI 367 AACGTT AA^CGTT
AflII 2630 CTTAAG C^TTAAG
Alw44I 1770 GTGCAC G^TGCAC
ApaLI 1770 GTGCAC G^TGCAC
AseI 3252 ATTAAT AT^TAAT
AsnI 3252 ATTAAT AT^TAAT
AvaI 1822 CTCGAG C^[CT]CG[GA]G
BbeI 2695 GGCGCC GGCGC^C
BbrPI 1478 CACGTG CAC^GTG
BciVI 2958 GTATCC GTATCC(6/5)
BfrI 2630 CTTAAG C^TTAAG
BglII 1818 AGATCT A^GATCT
BsaAI 1478 CACGTG [CT]AC^GT[GA]
BseRI 2042 GAGGAG GAGGAG(10/8)
BsoBI 1822 CTCGAG C^[CT]CG[GA]G
Bsp1407I 939 TGTACA T^GTACA
BspHI 1758 TCATGA T^CATGA
BsrDI 1890 CATTGC GCAATG(2/0)
BsrGI 939 TGTACA T^GTACA
BstAPI 552 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 371 TTCGAA TT^CGAA
BstUI 2555 CGCG CG^CG
DraI 2850 TTTAAA TTT^AAA
DsaI 128 CCATGG C^C[GA][CT]GG
HaeII 2695 GGCGCC [GA]GCGC^[CT]
HgaI 1593 GCGTC GACGC(5/10)
KasI 2695 GGCGCC G^GCGCC
MvnI 2555 CGCG CG^CG
NarI 2695 GGCGCC GG^CGCC
NcoI 128 CCATGG C^CATGG
NheI 2076 GCTAGC G^CTAGC
NspI 1279 ACATGC [GA]CATG^[CT]
NspV 371 TTCGAA TT^CGAA
PaeR7I 1822 CTCGAG C^TCGAG
PmaCI 1478 CACGTG CAC^GTG
PmlI 1478 CACGTG CAC^GTG
PshAI 1303 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3252 ATTAAT AT^TAAT
Psp1406I 367 AACGTT AA^CGTT
RcaI 1758 TCATGA T^CATGA
ScaI 2375 AGTACT AGT^ACT
SexAI 1025 ACCTGGT A^CC[AT]GGT
SfoI 2695 GGCGCC GGC^GCC
SfuI 371 TTCGAA TT^CGAA
SspBI 939 TGTACA T^GTACA
SspI 1832 AATATT AAT^ATT
StuI 1331 AGGCCT AGG^CCT
XbaI 909 TCTAGA T^CTAGA
XcmI 1684 CCAGAGTATGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1822 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 104 TCCGGA T^CCGGA
1347 TCCGGA
AcyI 79 GACGTC G[GA]^CG[CT]C
2695 GGCGCC
AspHI 22 GAGCAC G[AT]GC[AT]^C
1770 GTGCAC
BclI 212 TGATCA T^GATCA
3288 TGATCA
BmyI 22 GAGCAC G[AGT]GC[ACT]^C
1770 GTGCAC
BsaHI 79 GACGTC G[GA]^CG[CT]C
2695 GGCGCC
BsaI 470 GAGACC GGTCTC(1/5)
2334 GGTCTC
BsaWI 104 TCCGGA [AT]^CCGG[AT]
1347 TCCGGA
BseAI 104 TCCGGA T^CCGGA
1347 TCCGGA
BsgI 2713 CTGCAC GTGCAG(16/14)
1042 GTGCAG
BsiHKAI 22 GAGCAC G[AT]GC[AT]^C
1770 GTGCAC
BsmBI 2793 CGTCTC CGTCTC(1/5)
43 GAGACG
BsmI 1847 GCATTC GAATGC(1/-1)
2708 GCATTC
Bsp1286I 22 GAGCAC G[AGT]GC[ACT]^C
1770 GTGCAC
BspEI 104 TCCGGA T^CCGGA
1347 TCCGGA
BspMI 557 ACCTGC ACCTGC(4/8)
1243 GCAGGT
BstYI 1419 GGATCT [GA]^GATC[CT]
1818 AGATCT
CfoI 1922 GCGC GCG^C
2696 GCGC
FbaI 212 TGATCA T^GATCA
3288 TGATCA
HapII 105 CCGG C^CGG
1348 CCGG
HhaI 1922 GCGC GCG^C
2696 GCGC
Hin1I 79 GACGTC G[GA]^CG[CT]C
2695 GGCGCC
HinP1I 1922 GCGC G^CGC
2696 GCGC
HpaII 105 CCGG C^CGG
1348 CCGG
MfeI 943 CAATTG C^AATTG
1662 CAATTG
MflI 1419 GGATCT [GA]^GATC[CT]
1818 AGATCT
MroI 104 TCCGGA T^CCGGA
1347 TCCGGA
MslI 129 CATGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2194 CATCATTGTG
MspI 105 CCGG C^CGG
1348 CCGG
MunI 943 CAATTG C^AATTG
1662 CAATTG
NdeI 1325 CATATG CA^TATG
3095 CATATG
PflMI 1708 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2454 CCAGGCTCTGG
PstI 851 CTGCAG CTGCA^G
1414 CTGCAG
Tsp45I 2483 GTCAC ^GT[GC]AC
2950 GTCAC
Van91I 1708 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2454 CCAGGCTCTGG
XhoII 1419 GGATCT [GA]^GATC[CT]
1818 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanI BbsI
BbvI BfaI BglI BlpI BpmI
Bpu1102I BpuAI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI BstXI Cac8I CelII Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NsiI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I