##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccI AccII AclI AflII
AflIII AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbeI
BfrI BglII BlnI BsaBI BsaI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT22I FseI HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SexAI SfiI SfoI
SfuI SnaBI SpeI SspBI SspI
SwaI Tru9I Tth111I
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 366 GACGTC GACGT^C
AccIII 2199 TCCGGA T^CCGGA
AgeI 574 ACCGGT A^CCGGT
Asp700I 1505 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1407 GTGCTC G[AT]GC[AT]^C
AviII 1907 TGCGCA TGC^GCA
BbrPI 293 CACGTG CAC^GTG
BbsI 330 GTCTTC GAAGAC(2/6)
BclI 511 TGATCA T^GATCA
BlpI 540 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 540 GCTAAGC GC^T[ACGT]AGC
BpuAI 330 GTCTTC GAAGAC(2/6)
BsaAI 293 CACGTG [CT]AC^GT[GA]
BseAI 2199 TCCGGA T^CCGGA
BsiEI 701 CGACCG CG[GA][CT]^CG
BsiHKAI 1407 GTGCTC G[AT]GC[AT]^C
BsmBI 504 GAGACG CGTCTC(1/5)
BspEI 2199 TCCGGA T^CCGGA
BsrBI 1203 CCGCTC CCGCTC(-3/-3)
BssSI 1839 CTCGTG CACGAG(-5/-1)
BstAPI 1724 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 562 AGATCC [GA]^GATC[CT]
CelII 540 GCTAAGC GC^T[ACGT]AGC
DraIII 293 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 713 GACTATAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1849 GAATTC G^AATTC
EcoRV 234 GATATC GAT^ATC
FbaI 511 TGATCA T^GATCA
FspI 1907 TGCGCA TGC^GCA
HindIII 12 AAGCTT A^AGCTT
MflI 562 AGATCC [GA]^GATC[CT]
MroI 2199 TCCGGA T^CCGGA
NheI 242 GCTAGC G^CTAGC
PaeR7I 774 CTCGAG C^TCGAG
PflMI 938 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 574 ACCGGT A^CCGGT
PmaCI 293 CACGTG CAC^GTG
PmlI 293 CACGTG CAC^GTG
PshAI 2069 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 163 AGTACT AGT^ACT
SgrAI 573 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1615 CCCGGG CCC^GGG
SphI 520 GCATGC GCATG^C
TfiI 146 GATTC G^A[AT]TC
Van91I 938 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2223 TCTAGA T^CTAGA
XhoI 774 CTCGAG C^TCGAG
XhoII 562 AGATCC [GA]^GATC[CT]
XmaCI 1615 CCCGGG C^CCGGG
XmaI 1615 CCCGGG C^CCGGG
XmnI 1505 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 366 GACGTC G[GA]^CG[CT]C
1898 GGCGTC
AvaI 774 CTCGAG C^[CT]CG[GA]G
1615 CCCGGG
BalI 1327 TGGCCA TGG^CCA
2057 TGGCCA
BanI 1045 GGCACC G^G[CT][GA]CC
1347 GGCACC
BbvCI 863 CCTCAGC CCTCAGC(-5/-2)
1523 GCTGAGG
BcgI 520 GCATGCTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2169 GCAATAATTTCG
BciVI 1019 GGATAC GTATCC(6/5)
1919 GGATAC
BglI 1660 GCCTGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1780 GCCAGCAGGGC
BsaHI 366 GACGTC G[GA]^CG[CT]C
1898 GGCGTC
BsaWI 574 ACCGGT [AT]^CCGG[AT]
2199 TCCGGA
BsoBI 774 CTCGAG C^[CT]CG[GA]G
1615 CCCGGG
BsrFI 574 ACCGGT [GA]^CCGG[CT]
703 ACCGGC
Cfr10I 574 ACCGGT [GA]^CCGG[CT]
703 ACCGGC
EcoT14I 943 CCTTGG C^C[AT][AT]GG
1543 CCAAGG
HaeII 778 AGCGCC [GA]GCGC^[CT]
798 AGCGCC
Hin1I 366 GACGTC G[GA]^CG[CT]C
1898 GGCGTC
MluNI 1327 TGGCCA TGG^CCA
2057 TGGCCA
MscI 1327 TGGCCA TGG^CCA
2057 TGGCCA
SbfI 959 CCTGCAGG CCTGCA^GG
1305 CCTGCAGG
Sse8387I 959 CCTGCAGG CCTGCA^GG
1305 CCTGCAGG
StuI 940 AGGCCT AGG^CCT
1507 AGGCCT
StyI 943 CCTTGG C^C[AT][AT]GG
1543 CCAAGG
XcmI 1063 CCAGCAGTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1543 CCAAGGGGTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I