##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BamHI BbeI BbrPI BbvCI BciVI
BclI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
Hin1I KasI KpnI KspI MamI
MfeI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI Tth111I
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 789 CGCG CG^CG
AcsI 1693 AAATTC [GA]^AATT[CT]
AflII 1887 CTTAAG C^TTAAG
AflIII 788 ACGCGT A^C[GA][CT]GT
AlwNI 1643 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1693 AAATTC [GA]^AATT[CT]
BalI 1517 TGGCCA TGG^CCA
BanI 2191 GGCACC G^G[CT][GA]CC
BfrI 1887 CTTAAG C^TTAAG
BglI 295 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1090 TCCGGT [AT]^CCGG[AT]
BseRI 1768 GAGGAG GAGGAG(10/8)
BssSI 591 CTCGTG CACGAG(-5/-1)
BstUI 789 CGCG CG^CG
CfoI 2013 GCGC GCG^C
DraIII 896 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 783 GACGCACGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1517 TGGCCA [CT]^GGCC[GA]
EarI 1288 CTCTTC CTCTTC(1/4)
HaeII 2012 AGCGCC [GA]GCGC^[CT]
HhaI 2013 GCGC GCG^C
HinP1I 2013 GCGC G^CGC
HindIII 1416 AAGCTT A^AGCTT
HpaI 163 GTTAAC GTT^AAC
Ksp632I 1288 CTCTTC CTCTTC(1/4)
MluI 788 ACGCGT A^CGCGT
MluNI 1517 TGGCCA TGG^CCA
MscI 1517 TGGCCA TGG^CCA
MvnI 789 CGCG CG^CG
NspI 438 GCATGT [GA]CATG^[CT]
SacI 1434 GAGCTC GAGCT^C
SbfI 1227 CCTGCAGG CCTGCA^GG
SexAI 897 ACCAGGT A^CC[AT]GGT
SmaI 412 CCCGGG CCC^GGG
Sse8387I 1227 CCTGCAGG CCTGCA^GG
TaqI 1292 TCGA T^CGA
TthHB8I 1292 TCGA T^CGA
XmaCI 412 CCCGGG C^CCGGG
XmaI 412 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 1131 GATCC GGATC(4/5)
477 GGATC
BbsI 74 GAAGAC GAAGAC(2/6)
1798 GAAGAC
BcgI 959 CGAGCTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1066 GCAACATCATCG
BcnI 412 CCCGG CC^[GC]GG
413 CCGGG
BpuAI 74 GAAGAC GAAGAC(2/6)
1798 GAAGAC
BsgI 2355 CTGCAC GTGCAG(16/14)
2008 GTGCAG
BsmI 758 GCATTC GAATGC(1/-1)
2365 GCATTC
EcoNI 1287 CCTCTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1654 CCTAGGTGAGG
HapII 413 CCGG C^CGG
1091 CCGG
HgaI 783 GACGC GACGC(5/10)
790 GCGTC
HincII 163 GTTAAC GT[CT]^[GA]AC
2160 GTCAAC
HindII 163 GTTAAC GT[CT]^[GA]AC
2160 GTCAAC
HpaII 413 CCGG C^CGG
1091 CCGG
MspA1I 347 CCGCTG C[AC]G^C[GT]G
753 CAGCGG
MspI 413 CCGG C^CGG
1091 CCGG
NciI 412 CCCGG CC^[GC]GG
413 CCGGG
NheI 1530 GCTAGC G^CTAGC
2177 GCTAGC
PflMI 865 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
895 CCACCAGGTGG
PpuMI 811 AGGACCC [GA]G^G[AT]CC[CT]
1232 AGGACCT
Tsp509I 1694 AATT ^AATT
2038 AATT
Van91I 865 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
895 CCACCAGGTGG
XbaI 180 TCTAGA T^CTAGA
2435 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AciI AfaI AluI AspHI
AvaI AvaII AvrII BanII BbvI
BfaI BglII BlnI BmyI BpmI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TfiI Tru9I
TseI Tsp45I TspRI XhoII