##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AgeI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I BalI BanI BbeI BcgI
BglI BsaHI BseAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI Hin1I HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tsp509I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AhdI 948 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1494 GTGCAC G^TGCAC
AocI 926 CCTGAGG CC^T[ACGT]AGG
ApaI 1640 GGGCCC GGGCC^C
ApaLI 1494 GTGCAC G^TGCAC
AspEI 948 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 282 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1155 CCTAGG C^CTAGG
BbrPI 1497 CACGTG CAC^GTG
BciVI 2006 GTATCC GTATCC(6/5)
BclI 631 TGATCA T^GATCA
BlnI 1155 CCTAGG C^CTAGG
BsaBI 509 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 802 ACCGGA [AT]^CCGG[AT]
BsiEI 1760 CGATCG CG[GA][CT]^CG
Bsp1407I 1330 TGTACA T^GTACA
BspLU11I 1563 ACATGT A^CATGT
BsrBI 272 CCGCTC CCGCTC(-3/-3)
BsrGI 1330 TGTACA T^GTACA
BssSI 1050 CACGAG CACGAG(-5/-1)
BstXI 352 CCAATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 926 CCTGAGG CC^T[ACGT]AGG
DraIII 1363 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2057 CGGCCA [CT]^GGCC[GA]
Eam1105I 948 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 942 GAAGAG CTCTTC(1/4)
Eco81I 926 CCTGAGG CC^T[ACGT]AGG
EcoNI 455 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 631 TGATCA T^GATCA
HaeII 562 AGCGCC [GA]GCGC^[CT]
HgaI 2077 GCGTC GACGC(5/10)
HincII 291 GTTGAC GT[CT]^[GA]AC
HindII 291 GTTGAC GT[CT]^[GA]AC
HindIII 1766 AAGCTT A^AGCTT
Ksp632I 942 GAAGAG CTCTTC(1/4)
MamI 509 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 1077 CTCGAG C^TCGAG
PflFI 282 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1497 CACGTG CAC^GTG
PmlI 1497 CACGTG CAC^GTG
PvuI 1760 CGATCG CGAT^CG
ScaI 1990 AGTACT AGT^ACT
SmaI 1505 CCCGGG CCC^GGG
SphI 1344 GCATGC GCATG^C
SspBI 1330 TGTACA T^GTACA
Tth111I 282 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1077 CTCGAG C^TCGAG
XmaCI 1505 CCCGGG C^CCGGG
XmaI 1505 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 696 CTTAAG C^TTAAG
1851 CTTAAG
AflIII 1498 ACGTGT A^C[GA][CT]GT
1563 ACATGT
AvaI 1077 CTCGAG C^[CT]CG[GA]G
1505 CCCGGG
BbsI 767 GAAGAC GAAGAC(2/6)
999 GAAGAC
BbvCI 309 GCTGAGG CCTCAGC(-5/-2)
1900 GCTGAGG
BfrI 696 CTTAAG C^TTAAG
1851 CTTAAG
BglII 688 AGATCT A^GATCT
1100 AGATCT
BlpI 266 GCTGAGC GC^T[ACGT]AGC
274 GCTCAGC
Bpu1102I 266 GCTGAGC GC^T[ACGT]AGC
274 GCTCAGC
BpuAI 767 GAAGAC GAAGAC(2/6)
999 GAAGAC
BsaAI 1497 CACGTG [CT]AC^GT[GA]
2034 TACGTG
BsaI 648 GAGACC GGTCTC(1/5)
1074 GGTCTC
BsoBI 1077 CTCGAG C^[CT]CG[GA]G
1505 CCCGGG
BspMI 466 ACCTGC ACCTGC(4/8)
579 ACCTGC
BsrDI 377 CATTGC GCAATG(2/0)
599 CATTGC
CelII 266 GCTGAGC GC^T[ACGT]AGC
274 GCTCAGC
MaeII 1498 ACGT A^CGT
2035 ACGT
NcoI 424 CCATGG C^CATGG
1955 CCATGG
PflMI 419 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
560 CCAGCGCCTGG
StuI 452 AGGCCT AGG^CCT
1797 AGGCCT
Van91I 419 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
560 CCAGCGCCTGG
XcmI 247 CCAAACGCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1955 CCATGGATGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII AviII BamHI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI FokI FspI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I XhoII