################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI AspEI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 2028 GGTACC G^GTACC AccII 1640 CGCG CG^CG Asp718I 2028 GGTACC G^GTACC AspI 629 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 190 CTCGAG C^[CT]CG[GA]G BalI 460 TGGCCA TGG^CCA BbvCI 1819 CCTCAGC CCTCAGC(-5/-2) BglI 1469 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 937 AGATCT A^GATCT BsaI 193 GAGACC GGTCTC(1/5) BsgI 1700 CTGCAC GTGCAG(16/14) BsoBI 190 CTCGAG C^[CT]CG[GA]G BstUI 1640 CGCG CG^CG BstXI 1382 CCAAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 1321 TTTAAA TTT^AAA DsaI 505 CCATGG C^C[GA][CT]GG EarI 1287 CTCTTC CTCTTC(1/4) EcoNI 828 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 570 GGCGCT [GA]GCGC^[CT] HapII 340 CCGG C^CGG HindIII 1515 AAGCTT A^AGCTT HpaII 340 CCGG C^CGG KpnI 2028 GGTACC GGTAC^C Ksp632I 1287 CTCTTC CTCTTC(1/4) MaeII 839 ACGT A^CGT MluNI 460 TGGCCA TGG^CCA MscI 460 TGGCCA TGG^CCA MslI 530 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1922 CAGCTG C[AC]G^C[GT]G MspI 340 CCGG C^CGG MvnI 1640 CGCG CG^CG NcoI 505 CCATGG C^CATGG NheI 1449 GCTAGC G^CTAGC PaeR7I 190 CTCGAG C^TCGAG PflFI 629 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 437 GACTC GAGTC(4/5) PvuII 1922 CAGCTG CAG^CTG SacI 1590 GAGCTC GAGCT^C SbfI 296 CCTGCAGG CCTGCA^GG Sse8387I 296 CCTGCAGG CCTGCA^GG Tth111I 629 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1221 TCTAGA T^CTAGA XcmI 269 CCACATTTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 190 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 312 GACGCC G[GA]^CG[CT]C 681 GACGCC AflIII 622 ACATGT A^C[GA][CT]GT 957 ACATGT Alw44I 1167 GTGCAC G^TGCAC 1247 GTGCAC AlwNI 43 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 105 CAGAAGCTG ApaLI 1167 GTGCAC G^TGCAC 1247 GTGCAC Asp700I 155 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 227 GAATAATTTC BsaBI 480 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 557 GATCCTCATC BsaHI 312 GACGCC G[GA]^CG[CT]C 681 GACGCC BspLU11I 622 ACATGT A^CATGT 957 ACATGT BspMI 295 ACCTGC ACCTGC(4/8) 868 GCAGGT BsrDI 386 CATTGC GCAATG(2/0) 1995 CATTGC CfoI 571 GCGC GCG^C 1708 GCGC EaeI 4 CGGCCA [CT]^GGCC[GA] 460 TGGCCA FauI 89 CCCGC CCCGC(4/6) 655 CCCGC HgaI 312 GACGC GACGC(5/10) 681 GACGC HhaI 571 GCGC GCG^C 1708 GCGC Hin1I 312 GACGCC G[GA]^CG[CT]C 681 GACGCC HinP1I 571 GCGC G^CGC 1708 GCGC MamI 480 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 557 GATCCTCATC PpuMI 133 GGGACCT [GA]G^G[AT]CC[CT] 423 AGGACCC PstI 297 CTGCAG CTGCA^G 933 CTGCAG SfaNI 540 GATGC GCATC(5/9) 1824 GCATC SspI 715 AATATT AAT^ATT 2134 AATATT TfiI 1553 GATTC G^A[AT]TC 1610 GATTC XmnI 155 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 227 GAATAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI RsaI Sau3AI Sau96I ScaI ScrFI SfcI SmlI SspBI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII