##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KasI KspI MfeI MluI MroI
MunI NaeI NarI NciI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2028 GGTACC G^GTACC
AccII 1640 CGCG CG^CG
Asp718I 2028 GGTACC G^GTACC
AspI 629 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 190 CTCGAG C^[CT]CG[GA]G
BalI 460 TGGCCA TGG^CCA
BbvCI 1819 CCTCAGC CCTCAGC(-5/-2)
BglI 1469 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 937 AGATCT A^GATCT
BsaI 193 GAGACC GGTCTC(1/5)
BsgI 1700 CTGCAC GTGCAG(16/14)
BsoBI 190 CTCGAG C^[CT]CG[GA]G
BstUI 1640 CGCG CG^CG
BstXI 1382 CCAAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1321 TTTAAA TTT^AAA
DsaI 505 CCATGG C^C[GA][CT]GG
EarI 1287 CTCTTC CTCTTC(1/4)
EcoNI 828 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 570 GGCGCT [GA]GCGC^[CT]
HapII 340 CCGG C^CGG
HindIII 1515 AAGCTT A^AGCTT
HpaII 340 CCGG C^CGG
KpnI 2028 GGTACC GGTAC^C
Ksp632I 1287 CTCTTC CTCTTC(1/4)
MaeII 839 ACGT A^CGT
MluNI 460 TGGCCA TGG^CCA
MscI 460 TGGCCA TGG^CCA
MslI 530 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1922 CAGCTG C[AC]G^C[GT]G
MspI 340 CCGG C^CGG
MvnI 1640 CGCG CG^CG
NcoI 505 CCATGG C^CATGG
NheI 1449 GCTAGC G^CTAGC
PaeR7I 190 CTCGAG C^TCGAG
PflFI 629 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 437 GACTC GAGTC(4/5)
PvuII 1922 CAGCTG CAG^CTG
SacI 1590 GAGCTC GAGCT^C
SbfI 296 CCTGCAGG CCTGCA^GG
Sse8387I 296 CCTGCAGG CCTGCA^GG
Tth111I 629 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1221 TCTAGA T^CTAGA
XcmI 269 CCACATTTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 190 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 312 GACGCC G[GA]^CG[CT]C
681 GACGCC
AflIII 622 ACATGT A^C[GA][CT]GT
957 ACATGT
Alw44I 1167 GTGCAC G^TGCAC
1247 GTGCAC
AlwNI 43 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
105 CAGAAGCTG
ApaLI 1167 GTGCAC G^TGCAC
1247 GTGCAC
Asp700I 155 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
227 GAATAATTTC
BsaBI 480 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
557 GATCCTCATC
BsaHI 312 GACGCC G[GA]^CG[CT]C
681 GACGCC
BspLU11I 622 ACATGT A^CATGT
957 ACATGT
BspMI 295 ACCTGC ACCTGC(4/8)
868 GCAGGT
BsrDI 386 CATTGC GCAATG(2/0)
1995 CATTGC
CfoI 571 GCGC GCG^C
1708 GCGC
EaeI 4 CGGCCA [CT]^GGCC[GA]
460 TGGCCA
FauI 89 CCCGC CCCGC(4/6)
655 CCCGC
HgaI 312 GACGC GACGC(5/10)
681 GACGC
HhaI 571 GCGC GCG^C
1708 GCGC
Hin1I 312 GACGCC G[GA]^CG[CT]C
681 GACGCC
HinP1I 571 GCGC G^CGC
1708 GCGC
MamI 480 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
557 GATCCTCATC
PpuMI 133 GGGACCT [GA]G^G[AT]CC[CT]
423 AGGACCC
PstI 297 CTGCAG CTGCA^G
933 CTGCAG
SfaNI 540 GATGC GCATC(5/9)
1824 GCATC
SspI 715 AATATT AAT^ATT
2134 AATATT
TfiI 1553 GATTC G^A[AT]TC
1610 GATTC
XmnI 155 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
227 GAATAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI RsaI
Sau3AI Sau96I ScaI ScrFI SfcI
SmlI SspBI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII