################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## Acc65I AflII AflIII AgeI AhdI AscI Asp700I Asp718I AspEI AspI AvaI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BsaAI BsaBI BsiWI BsoBI Bsp1407I BspDI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI Eam1105I EcoO65I EcoRI EcoT22I FbaI FseI HpaI KpnI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SbfI SexAI SfiI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 2587 CGCG CG^CG AclI 446 AACGTT AA^CGTT AcsI 1444 GAATTT [GA]^AATT[CT] ApaI 1136 GGGCCC GGGCC^C ApoI 1444 GAATTT [GA]^AATT[CT] AseI 2888 ATTAAT AT^TAAT AsnI 2888 ATTAAT AT^TAAT AvrII 1474 CCTAGG C^CTAGG BalI 576 TGGCCA TGG^CCA BanI 2547 GGCGCC G^G[CT][GA]CC BbeI 2547 GGCGCC GGCGC^C BglI 1430 GCCAACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2491 AGATCT A^GATCT BlnI 1474 CCTAGG C^CTAGG BlpI 604 GCTAAGC GC^T[ACGT]AGC Bpu1102I 604 GCTAAGC GC^T[ACGT]AGC BsmI 490 GAATGC GAATGC(1/-1) BspHI 18 TCATGA T^CATGA BssSI 149 CTCGTG CACGAG(-5/-1) BstUI 2587 CGCG CG^CG CelII 604 GCTAAGC GC^T[ACGT]AGC DraIII 311 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1875 GACTTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1613 CGGCCG C^GGCCG EclXI 1613 CGGCCG C^GGCCG Eco52I 1613 CGGCCG C^GGCCG HindIII 1793 AAGCTT A^AGCTT KasI 2547 GGCGCC G^GCGCC MluNI 576 TGGCCA TGG^CCA MscI 576 TGGCCA TGG^CCA MslI 301 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 2587 CGCG CG^CG NaeI 2544 GCCGGC GCC^GGC NarI 2547 GGCGCC GG^CGCC NgoMIV 2544 GCCGGC G^CCGGC NspI 285 GCATGC [GA]CATG^[CT] PflMI 2751 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1354 AGGTCCC [GA]G^G[AT]CC[CT] PshBI 2888 ATTAAT AT^TAAT Psp1406I 446 AACGTT AA^CGTT RcaI 18 TCATGA T^CATGA ScaI 2211 AGTACT AGT^ACT SfoI 2547 GGCGCC GGC^GCC SgrAI 2543 CGCCGGCG C[GA]^CCGG[CT]G SphI 285 GCATGC GCATG^C Van91I 2751 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 733 TCTAGA T^CTAGA XcmI 1223 CCACTGCTTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AatII 797 GACGTC GACGT^C 2529 GACGTC AccI 655 GTCGAC GT^[AC][GT]AC 1909 GTCGAC AccIII 1215 TCCGGA T^CCGGA 1678 TCCGGA Alw44I 308 GTGCAC G^TGCAC 2829 GTGCAC Aor51HI 642 AGCGCT AGC^GCT 1373 AGCGCT ApaLI 308 GTGCAC G^TGCAC 2829 GTGCAC AviII 1022 TGCGCA TGC^GCA 1461 TGCGCA BsaI 629 GAGACC GGTCTC(1/5) 1056 GAGACC BseAI 1215 TCCGGA T^CCGGA 1678 TCCGGA BspEI 1215 TCCGGA T^CCGGA 1678 TCCGGA BsrBI 545 GAGCGG CCGCTC(-3/-3) 2800 GAGCGG BsrDI 1759 CATTGC GCAATG(2/0) 1954 CATTGC BsrFI 2544 GCCGGC [GA]^CCGG[CT] 2569 GCCGGT BstXI 111 CCAAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1440 CCAAGAATTTGG Cfr10I 2544 GCCGGC [GA]^CCGG[CT] 2569 GCCGGT EaeI 576 TGGCCA [CT]^GGCC[GA] 1613 CGGCCG Eco47III 642 AGCGCT AGC^GCT 1373 AGCGCT FspI 1022 TGCGCA TGC^GCA 1461 TGCGCA MroI 1215 TCCGGA T^CCGGA 1678 TCCGGA PstI 416 CTGCAG CTGCA^G 518 CTGCAG SacI 1280 GAGCTC GAGCT^C 2641 GAGCTC SalI 655 GTCGAC G^TCGAC 1909 GTCGAC SapI 1622 GAAGAGC GCTCTTC(1/4) 2351 GAAGAGC SmlI 755 CTTGAG C^T[CT][GA]AG 2120 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoNI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII