##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AflII AflIII AgeI AhdI
AscI Asp700I Asp718I AspEI AspI
AvaI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BsaAI BsaBI
BsiWI BsoBI Bsp1407I BspDI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI Eam1105I EcoO65I EcoRI EcoT22I
FbaI FseI HpaI KpnI KspI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SbfI SexAI SfiI SfuI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 2587 CGCG CG^CG
AclI 446 AACGTT AA^CGTT
AcsI 1444 GAATTT [GA]^AATT[CT]
ApaI 1136 GGGCCC GGGCC^C
ApoI 1444 GAATTT [GA]^AATT[CT]
AseI 2888 ATTAAT AT^TAAT
AsnI 2888 ATTAAT AT^TAAT
AvrII 1474 CCTAGG C^CTAGG
BalI 576 TGGCCA TGG^CCA
BanI 2547 GGCGCC G^G[CT][GA]CC
BbeI 2547 GGCGCC GGCGC^C
BglI 1430 GCCAACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2491 AGATCT A^GATCT
BlnI 1474 CCTAGG C^CTAGG
BlpI 604 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 604 GCTAAGC GC^T[ACGT]AGC
BsmI 490 GAATGC GAATGC(1/-1)
BspHI 18 TCATGA T^CATGA
BssSI 149 CTCGTG CACGAG(-5/-1)
BstUI 2587 CGCG CG^CG
CelII 604 GCTAAGC GC^T[ACGT]AGC
DraIII 311 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1875 GACTTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1613 CGGCCG C^GGCCG
EclXI 1613 CGGCCG C^GGCCG
Eco52I 1613 CGGCCG C^GGCCG
HindIII 1793 AAGCTT A^AGCTT
KasI 2547 GGCGCC G^GCGCC
MluNI 576 TGGCCA TGG^CCA
MscI 576 TGGCCA TGG^CCA
MslI 301 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 2587 CGCG CG^CG
NaeI 2544 GCCGGC GCC^GGC
NarI 2547 GGCGCC GG^CGCC
NgoMIV 2544 GCCGGC G^CCGGC
NspI 285 GCATGC [GA]CATG^[CT]
PflMI 2751 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1354 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2888 ATTAAT AT^TAAT
Psp1406I 446 AACGTT AA^CGTT
RcaI 18 TCATGA T^CATGA
ScaI 2211 AGTACT AGT^ACT
SfoI 2547 GGCGCC GGC^GCC
SgrAI 2543 CGCCGGCG C[GA]^CCGG[CT]G
SphI 285 GCATGC GCATG^C
Van91I 2751 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 733 TCTAGA T^CTAGA
XcmI 1223 CCACTGCTTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 797 GACGTC GACGT^C
2529 GACGTC
AccI 655 GTCGAC GT^[AC][GT]AC
1909 GTCGAC
AccIII 1215 TCCGGA T^CCGGA
1678 TCCGGA
Alw44I 308 GTGCAC G^TGCAC
2829 GTGCAC
Aor51HI 642 AGCGCT AGC^GCT
1373 AGCGCT
ApaLI 308 GTGCAC G^TGCAC
2829 GTGCAC
AviII 1022 TGCGCA TGC^GCA
1461 TGCGCA
BsaI 629 GAGACC GGTCTC(1/5)
1056 GAGACC
BseAI 1215 TCCGGA T^CCGGA
1678 TCCGGA
BspEI 1215 TCCGGA T^CCGGA
1678 TCCGGA
BsrBI 545 GAGCGG CCGCTC(-3/-3)
2800 GAGCGG
BsrDI 1759 CATTGC GCAATG(2/0)
1954 CATTGC
BsrFI 2544 GCCGGC [GA]^CCGG[CT]
2569 GCCGGT
BstXI 111 CCAAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1440 CCAAGAATTTGG
Cfr10I 2544 GCCGGC [GA]^CCGG[CT]
2569 GCCGGT
EaeI 576 TGGCCA [CT]^GGCC[GA]
1613 CGGCCG
Eco47III 642 AGCGCT AGC^GCT
1373 AGCGCT
FspI 1022 TGCGCA TGC^GCA
1461 TGCGCA
MroI 1215 TCCGGA T^CCGGA
1678 TCCGGA
PstI 416 CTGCAG CTGCA^G
518 CTGCAG
SacI 1280 GAGCTC GAGCT^C
2641 GAGCTC
SalI 655 GTCGAC G^TCGAC
1909 GTCGAC
SapI 1622 GAAGAGC GCTCTTC(1/4)
2351 GAAGAGC
SmlI 755 CTTGAG C^T[CT][GA]AG
2120 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI AspHI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
Eco81I EcoNI EcoO109I EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII