##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AhdI ApaI AscI Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BglI BglII BlnI BsaAI BsaHI
BseAI BsiEI BsiWI BspEI BssHII
Bst1107I BstZ17I CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HpaI KasI KpnI KspI
MaeII MluI MroI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
PacI PaeR7I PflMI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I StuI
SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AflIII 2358 ACATGT A^C[GA][CT]GT
AgeI 2900 ACCGGT A^CCGGT
AlwI 2382 GATCC GGATC(4/5)
Asp700I 930 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3094 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 226 CCCGGG C^[CT]CG[GA]G
BalI 2866 TGGCCA TGG^CCA
BanI 1064 GGTGCC G^G[CT][GA]CC
BsaBI 345 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2900 ACCGGT [AT]^CCGG[AT]
BsmBI 1373 GAGACG CGTCTC(1/5)
BsoBI 226 CCCGGG C^[CT]CG[GA]G
BspDI 600 ATCGAT AT^CGAT
BspLU11I 2358 ACATGT A^CATGT
BspMI 2542 GCAGGT ACCTGC(4/8)
BsrBI 746 CCGCTC CCGCTC(-3/-3)
BsrFI 2900 ACCGGT [GA]^CCGG[CT]
BstAPI 1147 GCACACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1290 TTCGAA TT^CGAA
BstEII 2532 GGTGACC G^GT[ACGT]ACC
BstPI 2532 GGTGACC G^GT[ACGT]ACC
BstXI 875 CCAGTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2381 AGATCC [GA]^GATC[CT]
Cfr10I 2900 ACCGGT [GA]^CCGG[CT]
ClaI 600 ATCGAT AT^CGAT
DpnII 2382 GATC ^GATC
DraII 824 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1475 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 824 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2532 GGTGACC G^GT[ACGT]ACC
EcoT14I 2066 CCAAGG C^C[AT][AT]GG
MamI 345 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 2382 GATC ^GATC
MfeI 565 CAATTG C^AATTG
MflI 2381 AGATCC [GA]^GATC[CT]
MluNI 2866 TGGCCA TGG^CCA
MscI 2866 TGGCCA TGG^CCA
MunI 565 CAATTG C^AATTG
NdeI 2024 CATATG CA^TATG
NdeII 2382 GATC ^GATC
NheI 669 GCTAGC G^CTAGC
NspV 1290 TTCGAA TT^CGAA
PflFI 3094 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2900 ACCGGT A^CCGGT
PpuMI 824 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1722 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 2382 GATC ^GATC
ScaI 877 AGTACT AGT^ACT
SfuI 1290 TTCGAA TT^CGAA
SmaI 226 CCCGGG CCC^GGG
SphI 149 GCATGC GCATG^C
StyI 2066 CCAAGG C^C[AT][AT]GG
Tth111I 3094 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1437 CCATTGTGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2381 AGATCC [GA]^GATC[CT]
XmaCI 226 CCCGGG C^CCGGG
XmaI 226 CCCGGG C^CCGGG
XmnI 930 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflII 2404 CTTAAG C^TTAAG
2462 CTTAAG
Alw44I 842 GTGCAC G^TGCAC
1442 GTGCAC
AocI 324 CCTTAGG CC^T[ACGT]AGG
816 CCTCAGG
Aor51HI 1574 AGCGCT AGC^GCT
2976 AGCGCT
ApaLI 842 GTGCAC G^TGCAC
1442 GTGCAC
AseI 174 ATTAAT AT^TAAT
2188 ATTAAT
AsnI 174 ATTAAT AT^TAAT
2188 ATTAAT
BfrI 2404 CTTAAG C^TTAAG
2462 CTTAAG
Bsp1407I 2160 TGTACA T^GTACA
2598 TGTACA
BspHI 629 TCATGA T^CATGA
3383 TCATGA
BsrDI 2906 CATTGC GCAATG(2/0)
3324 CATTGC
BsrGI 2160 TGTACA T^GTACA
2598 TGTACA
Bsu36I 324 CCTTAGG CC^T[ACGT]AGG
816 CCTCAGG
DsaI 2684 CCGTGG C^C[GA][CT]GG
2699 CCGTGG
EaeI 743 TGGCCG [CT]^GGCC[GA]
2866 TGGCCA
Eco47III 1574 AGCGCT AGC^GCT
2976 AGCGCT
Eco81I 324 CCTTAGG CC^T[ACGT]AGG
816 CCTCAGG
EcoRI 1000 GAATTC G^AATTC
1624 GAATTC
FauI 1334 GCGGG CCCGC(4/6)
1343 GCGGG
HaeII 1574 AGCGCT [GA]GCGC^[CT]
2976 AGCGCT
MspA1I 1167 CAGCTG C[AC]G^C[GT]G
3103 CAGCTG
NlaIV 36 GGAGCC GG[ACGT]^[ACGT]CC
1064 GGTGCC
NspI 149 GCATGC [GA]CATG^[CT]
2358 ACATGT
PshBI 174 ATTAAT AT^TAAT
2188 ATTAAT
PstI 23 CTGCAG CTGCA^G
1110 CTGCAG
PvuII 1167 CAGCTG CAG^CTG
3103 CAGCTG
RcaI 629 TCATGA T^CATGA
3383 TCATGA
SacI 2611 GAGCTC GAGCT^C
3387 GAGCTC
SapI 367 GAAGAGC GCTCTTC(1/4)
1303 GAAGAGC
SspBI 2160 TGTACA T^GTACA
2598 TGTACA
XbaI 73 TCTAGA T^CTAGA
961 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BssSI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr13I
DdeI DraI EarI EcoNI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboII MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NlaIII
PleI RsaI Sau96I ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I