##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BcnI BglII BlnI BpuAI BsaHI
BsiEI BsiWI BsmBI BspDI BspHI
BsrBI BsrFI BssHII BssSI BstAPI
BstEII BstPI BstUI BstYI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FauI FseI FspI
HgaI Hin1I KasI KpnI KspI
MfeI MflI MluI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfcI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1990 GTATAC GT^[AC][GT]AC
AccIII 838 TCCGGA T^CCGGA
AciI 250 GCGG CCGC(-3/-1)
AlwI 1808 GATCC GGATC(4/5)
AocI 2802 CCTTAGG CC^T[ACGT]AGG
BbvCI 2021 GCTGAGG CCTCAGC(-5/-2)
BclI 1609 TGATCA T^GATCA
BglI 1140 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2597 GGTCTC GGTCTC(1/5)
BseAI 838 TCCGGA T^CCGGA
Bsp1407I 2613 TGTACA T^GTACA
BspEI 838 TCCGGA T^CCGGA
BspLU11I 2472 ACATGT A^CATGT
BspMI 377 ACCTGC ACCTGC(4/8)
BsrGI 2613 TGTACA T^GTACA
Bst1107I 1990 GTATAC GTA^TAC
BstBI 138 TTCGAA TT^CGAA
BstZ17I 1990 GTATAC GTA^TAC
Bsu36I 2802 CCTTAGG CC^T[ACGT]AGG
CfoI 598 GCGC GCG^C
DsaI 354 CCGTGG C^C[GA][CT]GG
Eco81I 2802 CCTTAGG CC^T[ACGT]AGG
EcoNI 548 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1609 TGATCA T^GATCA
HaeII 597 AGCGCC [GA]GCGC^[CT]
HhaI 598 GCGC GCG^C
HinP1I 598 GCGC G^CGC
HincII 2738 GTTAAC GT[CT]^[GA]AC
HindII 2738 GTTAAC GT[CT]^[GA]AC
HpaI 2738 GTTAAC GTT^AAC
MroI 838 TCCGGA T^CCGGA
NspV 138 TTCGAA TT^CGAA
PflMI 1752 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1782 GACTC GAGTC(4/5)
SacI 2848 GAGCTC GAGCT^C
SapI 713 GAAGAGC GCTCTTC(1/4)
SfiI 1139 GGCCAAAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 138 TTCGAA TT^CGAA
SpeI 1883 ACTAGT A^CTAGT
SspBI 2613 TGTACA T^GTACA
StuI 2181 AGGCCT AGG^CCT
Van91I 1752 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 1998 AAATTC [GA]^AATT[CT]
2527 AAATTT
AflII 570 CTTAAG C^TTAAG
2582 CTTAAG
AflIII 2128 ACGTGT A^C[GA][CT]GT
2472 ACATGT
AlwNI 125 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
1638 CAGTTACTG
ApaI 1835 GGGCCC GGGCC^C
2147 GGGCCC
ApoI 1998 AAATTC [GA]^AATT[CT]
2527 AAATTT
Asp700I 864 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
966 GAATGGTTTC
AspHI 928 GAGCAC G[AT]GC[AT]^C
2848 GAGCTC
AvaI 319 CCCGAG C^[CT]CG[GA]G
651 CCCGAG
BfrI 570 CTTAAG C^TTAAG
2582 CTTAAG
BlpI 925 GCTGAGC GC^T[ACGT]AGC
1201 GCTAAGC
Bpu1102I 925 GCTGAGC GC^T[ACGT]AGC
1201 GCTAAGC
BsaAI 522 TACGTG [CT]AC^GT[GA]
2127 TACGTG
BsaBI 874 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1802 GATGATGATC
BsaWI 309 ACCGGA [AT]^CCGG[AT]
838 TCCGGA
BsiHKAI 928 GAGCAC G[AT]GC[AT]^C
2848 GAGCTC
BsmFI 2631 GGGAC GGGAC(10/14)
2834 GTCCC
BsmI 864 GAATGC GAATGC(1/-1)
868 GCATTC
BsoBI 319 CCCGAG C^[CT]CG[GA]G
651 CCCGAG
BstXI 960 CCAGCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2547 CCACTTTTGTGG
CelII 925 GCTGAGC GC^T[ACGT]AGC
1201 GCTAAGC
EcoT14I 328 CCTTGG C^C[AT][AT]GG
2657 CCTTGG
EcoT22I 866 ATGCAT ATGCA^T
2620 ATGCAT
HapII 310 CCGG C^CGG
839 CCGG
HpaII 310 CCGG C^CGG
839 CCGG
MamI 874 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1802 GATGATGATC
MspI 310 CCGG C^CGG
839 CCGG
NdeI 1282 CATATG CA^TATG
2617 CATATG
NsiI 866 ATGCAT ATGCA^T
2620 ATGCAT
NspI 2472 ACATGT [GA]CATG^[CT]
2622 GCATGT
PpuMI 323 AGGACCC [GA]G^G[AT]CC[CT]
820 AGGACCT
SspI 1667 AATATT AAT^ATT
1716 AATATT
StyI 328 CCTTGG C^C[AT][AT]GG
2657 CCTTGG
XmnI 864 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
966 GAATGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AfaI AluI AvaII BalI BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I