################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BciVI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BstBI BstEII BstPI CelII ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HaeII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 1643 GTATAC GT^[AC][GT]AC AflII 3156 CTTAAG C^TTAAG ApaI 609 GGGCCC GGGCC^C BamHI 1676 GGATCC G^GATCC BanI 1238 GGTGCC G^G[CT][GA]CC BbrPI 1143 CACGTG CAC^GTG BfrI 3156 CTTAAG C^TTAAG BglI 919 GCCTTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1143 CACGTG [CT]AC^GT[GA] Bsp1407I 1597 TGTACA T^GTACA BspHI 2085 TCATGA T^CATGA BsrFI 792 ACCGGC [GA]^CCGG[CT] BsrGI 1597 TGTACA T^GTACA BssHII 1511 GCGCGC G^CGCGC BssSI 1574 CACGAG CACGAG(-5/-1) Bst1107I 1643 GTATAC GTA^TAC BstXI 1648 CCATTCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1643 GTATAC GTA^TAC Cfr10I 792 ACCGGC [GA]^CCGG[CT] DraIII 3009 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 190 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3586 GAATTC G^AATTC EcoRV 585 GATATC GAT^ATC HincII 3901 GTCAAC GT[CT]^[GA]AC HindII 3901 GTCAAC GT[CT]^[GA]AC NcoI 1664 CCATGG C^CATGG NheI 3869 GCTAGC G^CTAGC PaeR7I 3553 CTCGAG C^TCGAG PflMI 1142 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1143 CACGTG CAC^GTG PmlI 1143 CACGTG CAC^GTG PshAI 3562 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2217 CTGCAG CTGCA^G RcaI 2085 TCATGA T^CATGA SapI 1622 GCTCTTC GCTCTTC(1/4) SphI 1475 GCATGC GCATG^C SspBI 1597 TGTACA T^GTACA SspI 2893 AATATT AAT^ATT Van91I 1142 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 151 TCTAGA T^CTAGA XhoI 3553 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 783 GACGCC G[GA]^CG[CT]C 1317 GACGCC AflIII 250 ACATGT A^C[GA][CT]GT 1693 ACATGT AocI 327 CCTGAGG CC^T[ACGT]AGG 800 CCTGAGG Asp700I 737 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3531 GAAACCTTTC BalI 123 TGGCCA TGG^CCA 385 TGGCCA BbvCI 1526 GCTGAGG CCTCAGC(-5/-2) 2010 GCTGAGG BcgI 2456 CGAGCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3232 GCACTGTCTTCG BsaHI 783 GACGCC G[GA]^CG[CT]C 1317 GACGCC BsaI 2282 GAGACC GGTCTC(1/5) 3398 GGTCTC BspLU11I 250 ACATGT A^CATGT 1693 ACATGT BstAPI 1387 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3929 GCAGCTTTTGC Bsu36I 327 CCTGAGG CC^T[ACGT]AGG 800 CCTGAGG DraI 2565 TTTAAA TTT^AAA 2876 TTTAAA DrdI 2312 GACTATTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3095 GACAGGGATGTC Eco81I 327 CCTGAGG CC^T[ACGT]AGG 800 CCTGAGG FauI 1472 CCCGC CCCGC(4/6) 1745 GCGGG Hin1I 783 GACGCC G[GA]^CG[CT]C 1317 GACGCC MluNI 123 TGGCCA TGG^CCA 385 TGGCCA MscI 123 TGGCCA TGG^CCA 385 TGGCCA StuI 1375 AGGCCT AGG^CCT 3148 AGGCCT XmnI 737 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3531 GAAACCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII