##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BciVI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BstBI BstEII BstPI
CelII ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FseI FspI HaeII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 1643 GTATAC GT^[AC][GT]AC
AflII 3156 CTTAAG C^TTAAG
ApaI 609 GGGCCC GGGCC^C
BamHI 1676 GGATCC G^GATCC
BanI 1238 GGTGCC G^G[CT][GA]CC
BbrPI 1143 CACGTG CAC^GTG
BfrI 3156 CTTAAG C^TTAAG
BglI 919 GCCTTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1143 CACGTG [CT]AC^GT[GA]
Bsp1407I 1597 TGTACA T^GTACA
BspHI 2085 TCATGA T^CATGA
BsrFI 792 ACCGGC [GA]^CCGG[CT]
BsrGI 1597 TGTACA T^GTACA
BssHII 1511 GCGCGC G^CGCGC
BssSI 1574 CACGAG CACGAG(-5/-1)
Bst1107I 1643 GTATAC GTA^TAC
BstXI 1648 CCATTCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1643 GTATAC GTA^TAC
Cfr10I 792 ACCGGC [GA]^CCGG[CT]
DraIII 3009 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 190 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3586 GAATTC G^AATTC
EcoRV 585 GATATC GAT^ATC
HincII 3901 GTCAAC GT[CT]^[GA]AC
HindII 3901 GTCAAC GT[CT]^[GA]AC
NcoI 1664 CCATGG C^CATGG
NheI 3869 GCTAGC G^CTAGC
PaeR7I 3553 CTCGAG C^TCGAG
PflMI 1142 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1143 CACGTG CAC^GTG
PmlI 1143 CACGTG CAC^GTG
PshAI 3562 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2217 CTGCAG CTGCA^G
RcaI 2085 TCATGA T^CATGA
SapI 1622 GCTCTTC GCTCTTC(1/4)
SphI 1475 GCATGC GCATG^C
SspBI 1597 TGTACA T^GTACA
SspI 2893 AATATT AAT^ATT
Van91I 1142 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 151 TCTAGA T^CTAGA
XhoI 3553 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 783 GACGCC G[GA]^CG[CT]C
1317 GACGCC
AflIII 250 ACATGT A^C[GA][CT]GT
1693 ACATGT
AocI 327 CCTGAGG CC^T[ACGT]AGG
800 CCTGAGG
Asp700I 737 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3531 GAAACCTTTC
BalI 123 TGGCCA TGG^CCA
385 TGGCCA
BbvCI 1526 GCTGAGG CCTCAGC(-5/-2)
2010 GCTGAGG
BcgI 2456 CGAGCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3232 GCACTGTCTTCG
BsaHI 783 GACGCC G[GA]^CG[CT]C
1317 GACGCC
BsaI 2282 GAGACC GGTCTC(1/5)
3398 GGTCTC
BspLU11I 250 ACATGT A^CATGT
1693 ACATGT
BstAPI 1387 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3929 GCAGCTTTTGC
Bsu36I 327 CCTGAGG CC^T[ACGT]AGG
800 CCTGAGG
DraI 2565 TTTAAA TTT^AAA
2876 TTTAAA
DrdI 2312 GACTATTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3095 GACAGGGATGTC
Eco81I 327 CCTGAGG CC^T[ACGT]AGG
800 CCTGAGG
FauI 1472 CCCGC CCCGC(4/6)
1745 GCGGG
Hin1I 783 GACGCC G[GA]^CG[CT]C
1317 GACGCC
MluNI 123 TGGCCA TGG^CCA
385 TGGCCA
MscI 123 TGGCCA TGG^CCA
385 TGGCCA
StuI 1375 AGGCCT AGG^CCT
3148 AGGCCT
XmnI 737 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3531 GAAACCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII