##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflIII AgeI Aor51HI AscI AseI
AsnI Asp718I AspI AviII AvrII
BbeI BbsI BbvCI BcgI BglI
BglII BlnI BpuAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I ClaI EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
FspI HaeII HgaI KasI KpnI
KspI MamI MluI MroI MvnI
NarI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 2859 GACGTC GACGT^C
AcyI 2859 GACGTC G[GA]^CG[CT]C
AflII 2529 CTTAAG C^TTAAG
AhdI 890 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2469 GTGCAC G^TGCAC
AocI 2494 CCTGAGG CC^T[ACGT]AGG
ApaLI 2469 GTGCAC G^TGCAC
Asp700I 1216 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 890 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1295 TGGCCA TGG^CCA
BamHI 390 GGATCC G^GATCC
BbrPI 2565 CACGTG CAC^GTG
BciVI 594 GGATAC GTATCC(6/5)
BclI 2037 TGATCA T^GATCA
BfrI 2529 CTTAAG C^TTAAG
BlpI 1647 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1647 GCTGAGC GC^T[ACGT]AGC
BsaHI 2859 GACGTC G[GA]^CG[CT]C
BsmI 2641 GAATGC GAATGC(1/-1)
BspHI 1370 TCATGA T^CATGA
BspMI 468 ACCTGC ACCTGC(4/8)
BsrDI 634 CATTGC GCAATG(2/0)
BsrFI 1201 GCCGGC [GA]^CCGG[CT]
BstXI 1070 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2494 CCTGAGG CC^T[ACGT]AGG
CelII 1647 GCTGAGC GC^T[ACGT]AGC
CfoI 12 GCGC GCG^C
Cfr10I 1201 GCCGGC [GA]^CCGG[CT]
CpoI 53 CGGTCCG CG^G[AT]CCG
DrdI 1461 GACCAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 890 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2494 CCTGAGG CC^T[ACGT]AGG
FbaI 2037 TGATCA T^GATCA
HhaI 12 GCGC GCG^C
Hin1I 2859 GACGTC G[GA]^CG[CT]C
HinP1I 12 GCGC G^CGC
MfeI 2242 CAATTG C^AATTG
MluNI 1295 TGGCCA TGG^CCA
MscI 1295 TGGCCA TGG^CCA
MunI 2242 CAATTG C^AATTG
NaeI 1201 GCCGGC GCC^GGC
NgoMIV 1201 GCCGGC G^CCGGC
NheI 2451 GCTAGC G^CTAGC
PflMI 1065 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2565 CACGTG CAC^GTG
PmlI 2565 CACGTG CAC^GTG
PpuMI 2814 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1773 CAGCTG CAG^CTG
RcaI 1370 TCATGA T^CATGA
RsrII 53 CGGTCCG CG^G[AT]CCG
SacI 62 GAGCTC GAGCT^C
SapI 616 GCTCTTC GCTCTTC(1/4)
SbfI 1703 CCTGCAGG CCTGCA^GG
ScaI 1413 AGTACT AGT^ACT
SphI 1496 GCATGC GCATG^C
Sse8387I 1703 CCTGCAGG CCTGCA^GG
Van91I 1065 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1216 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
ApaI 25 GGGCCC GGGCC^C
79 GGGCCC
AvaI 71 CTCGGG C^[CT]CG[GA]G
2300 CTCGGG
AvaII 54 GGTCC G^G[AT]CC
2815 GGACC
BcnI 875 CCGGG CC^[GC]GG
2063 CCCGG
BsaAI 1299 CACGTA [CT]AC^GT[GA]
2565 CACGTG
BsmBI 1469 CGTCTC CGTCTC(1/5)
2861 CGTCTC
BsoBI 71 CTCGGG C^[CT]CG[GA]G
2300 CTCGGG
BstAPI 1845 GCATGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2418 GCATCGTTTGC
BstEII 879 GGTAACC G^GT[ACGT]ACC
1175 GGTCACC
BstPI 879 GGTAACC G^GT[ACGT]ACC
1175 GGTCACC
BstYI 390 GGATCC [GA]^GATC[CT]
798 AGATCC
DraI 2221 TTTAAA TTT^AAA
2794 TTTAAA
DraIII 1026 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2023 CACAGGGTG
EaeI 368 CGGCCA [CT]^GGCC[GA]
1295 TGGCCA
EcoNI 124 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
168 CCTTCTTCAGG
EcoO65I 879 GGTAACC G^GT[ACGT]ACC
1175 GGTCACC
HincII 998 GTTAAC GT[CT]^[GA]AC
1567 GTTAAC
HindII 998 GTTAAC GT[CT]^[GA]AC
1567 GTTAAC
HpaI 998 GTTAAC GTT^AAC
1567 GTTAAC
MflI 390 GGATCC [GA]^GATC[CT]
798 AGATCC
NciI 875 CCGGG CC^[GC]GG
2063 CCCGG
NcoI 1070 CCATGG C^CATGG
2663 CCATGG
TaqI 477 TCGA T^CGA
1047 TCGA
TthHB8I 477 TCGA T^CGA
1047 TCGA
XcmI 1682 CCAAGCTGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2476 CCATCAGCCCCTTGG
XhoII 390 GGATCC [GA]^GATC[CT]
798 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI BanI BanII
BbvI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI