##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      ApaI        
ApaLI       AscI        Asp718I     AspEI       AvaI        
AvrII       BalI        BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BspHI       
BsrBI       BsrFI       BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
CelII       Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco52I      EcoO109I    EcoO65I     
EcoRV       EcoT22I     FauI        FbaI        FseI        
Hin1I       KasI        KpnI        KspI        MaeII       
MluI        MluNI       MroI        MscI        MvnI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PpuMI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    StuI        SwaI        
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AflIII       3180  ACATGT               A^C[GA][CT]GT
AocI         2778  CCTAAGG              CC^T[ACGT]AGG
Aor51HI      2346  AGCGCT               AGC^GCT
AspHI        3470  GAGCTC               G[AT]GC[AT]^C
AspI          353  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII        1438  GGACC                G^G[AT]CC
AviII         808  TGCGCA               TGC^GCA
BamHI        1841  GGATCC               G^GATCC
BanII        3470  GAGCTC               G[GA]GC[CT]^C
BglII         302  AGATCT               A^GATCT
BpmI         1089  CTCCAG               CTGGAG(16/14)
BsaBI        2930  GATAGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI      3470  GAGCTC               G[AT]GC[AT]^C
BspLU11I     3180  ACATGT               A^CATGT
BspMI        2320  ACCTGC               ACCTGC(4/8)
Bsu36I       2778  CCTAAGG              CC^T[ACGT]AGG
Eco47III     2346  AGCGCT               AGC^GCT
Eco81I       2778  CCTAAGG              CC^T[ACGT]AGG
EcoNI        2444  CCTGAAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2380  GAATTC               G^AATTC
FspI          808  TGCGCA               TGC^GCA
HaeII        2346  AGCGCT               [GA]GCGC^[CT]
HapII          48  CCGG                 C^CGG
HgaI         2094  GCGTC                GACGC(5/10)
HindIII      1593  AAGCTT               A^AGCTT
HpaI          782  GTTAAC               GTT^AAC
HpaII          48  CCGG                 C^CGG
MamI         2930  GATAGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1813  CAATTG               C^AATTG
MspI           48  CCGG                 C^CGG
MunI         1813  CAATTG               C^AATTG
PflFI         353  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1277  CCAGGAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI         3470  GAGCTC               GAGCT^C
SspI         1806  AATATT               AAT^ATT
Tth111I       353  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1277  CCAGGAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI         3450  GTATAC               GT^[AC][GT]AC
             3660  GTATAC              
AflII        3415  CTTAAG               C^TTAAG
             3445  CTTAAG              
Asp700I       747  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2376  GAAAGAATTC          
BfrI         3415  CTTAAG               C^TTAAG
             3445  CTTAAG              
BmyI         3470  GAGCTC               G[AGT]GC[ACT]^C
             3711  GTGCCC              
BsaJI         520  CCAAGG               C^C[ACGT][ACGT]GG
             1070  CCAAGG              
BsgI         3651  CTGCAC               GTGCAG(16/14)
             1614  GTGCAG              
Bsp1286I     3470  GAGCTC               G[AGT]GC[ACT]^C
             3711  GTGCCC              
Bsp1407I      270  TGTACA               T^GTACA
              792  TGTACA              
BsrDI        2687  CATTGC               GCAATG(2/0)
             3510  CATTGC              
BsrGI         270  TGTACA               T^GTACA
              792  TGTACA              
Bst1107I     3450  GTATAC               GTA^TAC
             3660  GTATAC              
BstZ17I      3450  GTATAC               GTA^TAC
             3660  GTATAC              
Cfr13I       1438  GGACC                G^G[ACGT]CC
             3007  GGGCC               
EcoT14I       520  CCAAGG               C^C[AT][AT]GG
             1070  CCAAGG              
HphI         1344  GGTGA                GGTGA(8/7)
              755  TCACC               
NdeI         1949  CATATG               CA^TATG
             3048  CATATG              
PshAI        3234  GACTATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3569  GACTAGAGTC          
PstI          143  CTGCAG               CTGCA^G
             1417  CTGCAG              
Sau96I       1438  GGACC                G^G[ACGT]CC
             3007  GGGCC               
SexAI         249  ACCTGGT              A^CC[AT]GGT
              798  ACCAGGT             
SpeI         1098  ACTAGT               A^CTAGT
             1253  ACTAGT              
SspBI         270  TGTACA               T^GTACA
              792  TGTACA              
StyI          520  CCAAGG               C^C[AT][AT]GG
             1070  CCAAGG              
TaqI         2601  TCGA                 T^CGA
             3739  TCGA                
Tsp45I        316  GTGAC                ^GT[GC]AC
             3576  GTCAC               
TthHB8I      2601  TCGA                 T^CGA
             3739  TCGA                
XmnI          747  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2376  GAAAGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AseI        AsnI        BbsI        
BbvI        BfaI        BpuAI       BsaI        BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        DdeI        DpnII       DraI        
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HincII      HindII      HinfI       
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PshBI       PvuII       
RsaI        Sau3AI      ScaI        ScrFI       SfaNI       
SfcI        SmlI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XhoII