##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I ApaI
ApaLI AscI Asp718I AspEI AvaI
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco52I EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
Hin1I KasI KpnI KspI MaeII
MluI MluNI MroI MscI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI SwaI
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflIII 3180 ACATGT A^C[GA][CT]GT
AocI 2778 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2346 AGCGCT AGC^GCT
AspHI 3470 GAGCTC G[AT]GC[AT]^C
AspI 353 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1438 GGACC G^G[AT]CC
AviII 808 TGCGCA TGC^GCA
BamHI 1841 GGATCC G^GATCC
BanII 3470 GAGCTC G[GA]GC[CT]^C
BglII 302 AGATCT A^GATCT
BpmI 1089 CTCCAG CTGGAG(16/14)
BsaBI 2930 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 3470 GAGCTC G[AT]GC[AT]^C
BspLU11I 3180 ACATGT A^CATGT
BspMI 2320 ACCTGC ACCTGC(4/8)
Bsu36I 2778 CCTAAGG CC^T[ACGT]AGG
Eco47III 2346 AGCGCT AGC^GCT
Eco81I 2778 CCTAAGG CC^T[ACGT]AGG
EcoNI 2444 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2380 GAATTC G^AATTC
FspI 808 TGCGCA TGC^GCA
HaeII 2346 AGCGCT [GA]GCGC^[CT]
HapII 48 CCGG C^CGG
HgaI 2094 GCGTC GACGC(5/10)
HindIII 1593 AAGCTT A^AGCTT
HpaI 782 GTTAAC GTT^AAC
HpaII 48 CCGG C^CGG
MamI 2930 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1813 CAATTG C^AATTG
MspI 48 CCGG C^CGG
MunI 1813 CAATTG C^AATTG
PflFI 353 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1277 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 3470 GAGCTC GAGCT^C
SspI 1806 AATATT AAT^ATT
Tth111I 353 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1277 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 3450 GTATAC GT^[AC][GT]AC
3660 GTATAC
AflII 3415 CTTAAG C^TTAAG
3445 CTTAAG
Asp700I 747 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2376 GAAAGAATTC
BfrI 3415 CTTAAG C^TTAAG
3445 CTTAAG
BmyI 3470 GAGCTC G[AGT]GC[ACT]^C
3711 GTGCCC
BsaJI 520 CCAAGG C^C[ACGT][ACGT]GG
1070 CCAAGG
BsgI 3651 CTGCAC GTGCAG(16/14)
1614 GTGCAG
Bsp1286I 3470 GAGCTC G[AGT]GC[ACT]^C
3711 GTGCCC
Bsp1407I 270 TGTACA T^GTACA
792 TGTACA
BsrDI 2687 CATTGC GCAATG(2/0)
3510 CATTGC
BsrGI 270 TGTACA T^GTACA
792 TGTACA
Bst1107I 3450 GTATAC GTA^TAC
3660 GTATAC
BstZ17I 3450 GTATAC GTA^TAC
3660 GTATAC
Cfr13I 1438 GGACC G^G[ACGT]CC
3007 GGGCC
EcoT14I 520 CCAAGG C^C[AT][AT]GG
1070 CCAAGG
HphI 1344 GGTGA GGTGA(8/7)
755 TCACC
NdeI 1949 CATATG CA^TATG
3048 CATATG
PshAI 3234 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3569 GACTAGAGTC
PstI 143 CTGCAG CTGCA^G
1417 CTGCAG
Sau96I 1438 GGACC G^G[ACGT]CC
3007 GGGCC
SexAI 249 ACCTGGT A^CC[AT]GGT
798 ACCAGGT
SpeI 1098 ACTAGT A^CTAGT
1253 ACTAGT
SspBI 270 TGTACA T^GTACA
792 TGTACA
StyI 520 CCAAGG C^C[AT][AT]GG
1070 CCAAGG
TaqI 2601 TCGA T^CGA
3739 TCGA
Tsp45I 316 GTGAC ^GT[GC]AC
3576 GTCAC
TthHB8I 2601 TCGA T^CGA
3739 TCGA
XmnI 747 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2376 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI BbsI
BbvI BfaI BpuAI BsaI BseRI
BsiYI BslI BsmAI BsmFI BsmI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI DdeI DpnII DraI
EarI EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HinfI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI PvuII
RsaI Sau3AI ScaI ScrFI SfaNI
SfcI SmlI TfiI Tru9I TseI
Tsp509I TspRI XhoII