################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I ApaI ApaLI AscI Asp718I AspEI AvaI AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI Hin1I KasI KpnI KspI MaeII MluI MluNI MroI MscI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AflIII 3180 ACATGT A^C[GA][CT]GT AocI 2778 CCTAAGG CC^T[ACGT]AGG Aor51HI 2346 AGCGCT AGC^GCT AspHI 3470 GAGCTC G[AT]GC[AT]^C AspI 353 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1438 GGACC G^G[AT]CC AviII 808 TGCGCA TGC^GCA BamHI 1841 GGATCC G^GATCC BanII 3470 GAGCTC G[GA]GC[CT]^C BglII 302 AGATCT A^GATCT BpmI 1089 CTCCAG CTGGAG(16/14) BsaBI 2930 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 3470 GAGCTC G[AT]GC[AT]^C BspLU11I 3180 ACATGT A^CATGT BspMI 2320 ACCTGC ACCTGC(4/8) Bsu36I 2778 CCTAAGG CC^T[ACGT]AGG Eco47III 2346 AGCGCT AGC^GCT Eco81I 2778 CCTAAGG CC^T[ACGT]AGG EcoNI 2444 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2380 GAATTC G^AATTC FspI 808 TGCGCA TGC^GCA HaeII 2346 AGCGCT [GA]GCGC^[CT] HapII 48 CCGG C^CGG HgaI 2094 GCGTC GACGC(5/10) HindIII 1593 AAGCTT A^AGCTT HpaI 782 GTTAAC GTT^AAC HpaII 48 CCGG C^CGG MamI 2930 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1813 CAATTG C^AATTG MspI 48 CCGG C^CGG MunI 1813 CAATTG C^AATTG PflFI 353 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1277 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 3470 GAGCTC GAGCT^C SspI 1806 AATATT AAT^ATT Tth111I 353 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1277 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 3450 GTATAC GT^[AC][GT]AC 3660 GTATAC AflII 3415 CTTAAG C^TTAAG 3445 CTTAAG Asp700I 747 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2376 GAAAGAATTC BfrI 3415 CTTAAG C^TTAAG 3445 CTTAAG BmyI 3470 GAGCTC G[AGT]GC[ACT]^C 3711 GTGCCC BsaJI 520 CCAAGG C^C[ACGT][ACGT]GG 1070 CCAAGG BsgI 3651 CTGCAC GTGCAG(16/14) 1614 GTGCAG Bsp1286I 3470 GAGCTC G[AGT]GC[ACT]^C 3711 GTGCCC Bsp1407I 270 TGTACA T^GTACA 792 TGTACA BsrDI 2687 CATTGC GCAATG(2/0) 3510 CATTGC BsrGI 270 TGTACA T^GTACA 792 TGTACA Bst1107I 3450 GTATAC GTA^TAC 3660 GTATAC BstZ17I 3450 GTATAC GTA^TAC 3660 GTATAC Cfr13I 1438 GGACC G^G[ACGT]CC 3007 GGGCC EcoT14I 520 CCAAGG C^C[AT][AT]GG 1070 CCAAGG HphI 1344 GGTGA GGTGA(8/7) 755 TCACC NdeI 1949 CATATG CA^TATG 3048 CATATG PshAI 3234 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3569 GACTAGAGTC PstI 143 CTGCAG CTGCA^G 1417 CTGCAG Sau96I 1438 GGACC G^G[ACGT]CC 3007 GGGCC SexAI 249 ACCTGGT A^CC[AT]GGT 798 ACCAGGT SpeI 1098 ACTAGT A^CTAGT 1253 ACTAGT SspBI 270 TGTACA T^GTACA 792 TGTACA StyI 520 CCAAGG C^C[AT][AT]GG 1070 CCAAGG TaqI 2601 TCGA T^CGA 3739 TCGA Tsp45I 316 GTGAC ^GT[GC]AC 3576 GTCAC TthHB8I 2601 TCGA T^CGA 3739 TCGA XmnI 747 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2376 GAAAGAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI BbsI BbvI BfaI BpuAI BsaI BseRI BsiYI BslI BsmAI BsmFI BsmI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI PvuII RsaI Sau3AI ScaI ScrFI SfaNI SfcI SmlI TfiI Tru9I TseI Tsp509I TspRI XhoII