################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## Acc65I AclI AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AviII AvrII BalI BanI BanII BbeI BbrPI BciVI BglI BlnI BsiWI BsmBI Bsp1407I BspHI BspLU11I BsrGI Bst1107I BstBI BstEII BstPI BstZ17I CpoI DraII DraIII DrdI Eam1105I Eco47III EcoNI EcoO109I EcoO65I EcoRV EcoT14I FseI FspI HaeII KasI KpnI MfeI MluNI MscI MunI NaeI NarI NcoI NgoMIV NotI NruI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I RcaI RsrII SacI SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StyI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 3024 GACGTC GACGT^C AccI 2436 GTCTAC GT^[AC][GT]AC AccIII 1533 TCCGGA T^CCGGA AcyI 3024 GACGTC G[GA]^CG[CT]C AflIII 3055 ACGCGT A^C[GA][CT]GT Alw44I 2673 GTGCAC G^TGCAC AocI 853 CCTGAGG CC^T[ACGT]AGG ApaLI 2673 GTGCAC G^TGCAC AseI 658 ATTAAT AT^TAAT AsnI 658 ATTAAT AT^TAAT Asp700I 492 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 212 GGATCC G^GATCC BbvCI 219 CCTCAGC CCTCAGC(-5/-2) BcgI 3340 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 429 GCTAAGC GC^T[ACGT]AGC Bpu1102I 429 GCTAAGC GC^T[ACGT]AGC BsaHI 3024 GACGTC G[GA]^CG[CT]C BseAI 1533 TCCGGA T^CCGGA BseRI 2833 GAGGAG GAGGAG(10/8) BspDI 343 ATCGAT AT^CGAT BspEI 1533 TCCGGA T^CCGGA BsrFI 161 GCCGGT [GA]^CCGG[CT] BssSI 1746 CACGAG CACGAG(-5/-1) Bsu36I 853 CCTGAGG CC^T[ACGT]AGG CelII 429 GCTAAGC GC^T[ACGT]AGC Cfr10I 161 GCCGGT [GA]^CCGG[CT] ClaI 343 ATCGAT AT^CGAT DraI 3616 TTTAAA TTT^AAA DsaI 120 CCGCGG C^C[GA][CT]GG Eco81I 853 CCTGAGG CC^T[ACGT]AGG EcoRI 848 GAATTC G^AATTC EcoT22I 3101 ATGCAT ATGCA^T Hin1I 3024 GACGTC G[GA]^CG[CT]C HindIII 432 AAGCTT A^AGCTT HpaI 2473 GTTAAC GTT^AAC KspI 120 CCGCGG CCGC^GG MluI 3055 ACGCGT A^CGCGT MroI 1533 TCCGGA T^CCGGA NdeI 3881 CATATG CA^TATG NheI 3532 GCTAGC G^CTAGC NsiI 3101 ATGCAT ATGCA^T PshAI 1429 GACGCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 658 ATTAAT AT^TAAT PvuI 626 CGATCG CGAT^CG SacII 120 CCGCGG CCGC^GG StuI 2145 AGGCCT AGG^CCT XmnI 492 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AspHI 610 GAGCAC G[AT]GC[AT]^C 2673 GTGCAC AspI 448 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2956 GACGTAGTC AvaI 9 CTCGGG C^[CT]CG[GA]G 48 CCCGAG BbsI 1008 GAAGAC GAAGAC(2/6) 1387 GAAGAC BclI 1407 TGATCA T^GATCA 2361 TGATCA BcnI 94 CCCGG CC^[GC]GG 209 CCGGG BmyI 610 GAGCAC G[AGT]GC[ACT]^C 2673 GTGCAC BpuAI 1008 GAAGAC GAAGAC(2/6) 1387 GAAGAC BsaAI 992 TACGTG [CT]AC^GT[GA] 3049 CACGTA BsaBI 336 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2923 GATCTGGATC BsaWI 1533 TCCGGA [AT]^CCGG[AT] 1623 ACCGGA BsiHKAI 610 GAGCAC G[AT]GC[AT]^C 2673 GTGCAC BsoBI 9 CTCGGG C^[CT]CG[GA]G 48 CCCGAG Bsp1286I 610 GAGCAC G[AGT]GC[ACT]^C 2673 GTGCAC BsrDI 532 GCAATG GCAATG(2/0) 2825 GCAATG BssHII 224 GCGCGC G^CGCGC 2561 GCGCGC BstXI 560 CCATGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1477 CCAGAAAAATGG EagI 153 CGGCCG C^GGCCG 2804 CGGCCG EclXI 153 CGGCCG C^GGCCG 2804 CGGCCG Eco52I 153 CGGCCG C^GGCCG 2804 CGGCCG FbaI 1407 TGATCA T^GATCA 2361 TGATCA MamI 336 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2923 GATCTGGATC NciI 94 CCCGG CC^[GC]GG 209 CCGGG PflFI 448 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2956 GACGTAGTC SapI 2752 GAAGAGC GCTCTTC(1/4) 3076 GAAGAGC ScaI 3010 AGTACT AGT^ACT 3797 AGTACT SpeI 259 ACTAGT A^CTAGT 1017 ACTAGT SspI 3208 AATATT AAT^ATT 3229 AATATT Tth111I 448 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2956 GACGTAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AluI AlwI AlwNI ApoI AvaII BbvI BfaI BfrI BglII BpmI BsaI BsaJI BsgI BsiEI BsiYI BslI BsmAI BsmFI BsmI BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII