##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AclI AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AviII
AvrII BalI BanI BanII BbeI
BbrPI BciVI BglI BlnI BsiWI
BsmBI Bsp1407I BspHI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraII DraIII DrdI Eam1105I
Eco47III EcoNI EcoO109I EcoO65I EcoRV
EcoT14I FseI FspI HaeII KasI
KpnI MfeI MluNI MscI MunI
NaeI NarI NcoI NgoMIV NotI
NruI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I RcaI RsrII SacI
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI StyI SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 3024 GACGTC GACGT^C
AccI 2436 GTCTAC GT^[AC][GT]AC
AccIII 1533 TCCGGA T^CCGGA
AcyI 3024 GACGTC G[GA]^CG[CT]C
AflIII 3055 ACGCGT A^C[GA][CT]GT
Alw44I 2673 GTGCAC G^TGCAC
AocI 853 CCTGAGG CC^T[ACGT]AGG
ApaLI 2673 GTGCAC G^TGCAC
AseI 658 ATTAAT AT^TAAT
AsnI 658 ATTAAT AT^TAAT
Asp700I 492 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 212 GGATCC G^GATCC
BbvCI 219 CCTCAGC CCTCAGC(-5/-2)
BcgI 3340 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 429 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 429 GCTAAGC GC^T[ACGT]AGC
BsaHI 3024 GACGTC G[GA]^CG[CT]C
BseAI 1533 TCCGGA T^CCGGA
BseRI 2833 GAGGAG GAGGAG(10/8)
BspDI 343 ATCGAT AT^CGAT
BspEI 1533 TCCGGA T^CCGGA
BsrFI 161 GCCGGT [GA]^CCGG[CT]
BssSI 1746 CACGAG CACGAG(-5/-1)
Bsu36I 853 CCTGAGG CC^T[ACGT]AGG
CelII 429 GCTAAGC GC^T[ACGT]AGC
Cfr10I 161 GCCGGT [GA]^CCGG[CT]
ClaI 343 ATCGAT AT^CGAT
DraI 3616 TTTAAA TTT^AAA
DsaI 120 CCGCGG C^C[GA][CT]GG
Eco81I 853 CCTGAGG CC^T[ACGT]AGG
EcoRI 848 GAATTC G^AATTC
EcoT22I 3101 ATGCAT ATGCA^T
Hin1I 3024 GACGTC G[GA]^CG[CT]C
HindIII 432 AAGCTT A^AGCTT
HpaI 2473 GTTAAC GTT^AAC
KspI 120 CCGCGG CCGC^GG
MluI 3055 ACGCGT A^CGCGT
MroI 1533 TCCGGA T^CCGGA
NdeI 3881 CATATG CA^TATG
NheI 3532 GCTAGC G^CTAGC
NsiI 3101 ATGCAT ATGCA^T
PshAI 1429 GACGCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 658 ATTAAT AT^TAAT
PvuI 626 CGATCG CGAT^CG
SacII 120 CCGCGG CCGC^GG
StuI 2145 AGGCCT AGG^CCT
XmnI 492 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 610 GAGCAC G[AT]GC[AT]^C
2673 GTGCAC
AspI 448 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2956 GACGTAGTC
AvaI 9 CTCGGG C^[CT]CG[GA]G
48 CCCGAG
BbsI 1008 GAAGAC GAAGAC(2/6)
1387 GAAGAC
BclI 1407 TGATCA T^GATCA
2361 TGATCA
BcnI 94 CCCGG CC^[GC]GG
209 CCGGG
BmyI 610 GAGCAC G[AGT]GC[ACT]^C
2673 GTGCAC
BpuAI 1008 GAAGAC GAAGAC(2/6)
1387 GAAGAC
BsaAI 992 TACGTG [CT]AC^GT[GA]
3049 CACGTA
BsaBI 336 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2923 GATCTGGATC
BsaWI 1533 TCCGGA [AT]^CCGG[AT]
1623 ACCGGA
BsiHKAI 610 GAGCAC G[AT]GC[AT]^C
2673 GTGCAC
BsoBI 9 CTCGGG C^[CT]CG[GA]G
48 CCCGAG
Bsp1286I 610 GAGCAC G[AGT]GC[ACT]^C
2673 GTGCAC
BsrDI 532 GCAATG GCAATG(2/0)
2825 GCAATG
BssHII 224 GCGCGC G^CGCGC
2561 GCGCGC
BstXI 560 CCATGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1477 CCAGAAAAATGG
EagI 153 CGGCCG C^GGCCG
2804 CGGCCG
EclXI 153 CGGCCG C^GGCCG
2804 CGGCCG
Eco52I 153 CGGCCG C^GGCCG
2804 CGGCCG
FbaI 1407 TGATCA T^GATCA
2361 TGATCA
MamI 336 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2923 GATCTGGATC
NciI 94 CCCGG CC^[GC]GG
209 CCGGG
PflFI 448 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2956 GACGTAGTC
SapI 2752 GAAGAGC GCTCTTC(1/4)
3076 GAAGAGC
ScaI 3010 AGTACT AGT^ACT
3797 AGTACT
SpeI 259 ACTAGT A^CTAGT
1017 ACTAGT
SspI 3208 AATATT AAT^ATT
3229 AATATT
Tth111I 448 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2956 GACGTAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI AlwNI ApoI AvaII
BbvI BfaI BfrI BglII BpmI
BsaI BsaJI BsgI BsiEI BsiYI
BslI BsmAI BsmFI BsmI BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII