################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII BciVI BclI BfrI BglII BsaWI BseAI BsiWI BsmBI BsmI BspEI BspHI BspLU11I BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII KpnI KspI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 92 AAATTC [GA]^AATT[CT] AflIII 239 ACACGT A^C[GA][CT]GT ApoI 92 AAATTC [GA]^AATT[CT] Asp700I 1914 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1997 CCCGGG C^[CT]CG[GA]G BalI 1460 TGGCCA TGG^CCA BamHI 1603 GGATCC G^GATCC BbrPI 240 CACGTG CAC^GTG BbvCI 1761 CCTCAGC CCTCAGC(-5/-2) BcgI 1315 CGAAGAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1701 GCCTAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 240 CACGTG [CT]AC^GT[GA] BsaBI 1026 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 776 CGGTCG CG[GA][CT]^CG BsoBI 1997 CCCGGG C^[CT]CG[GA]G Bsp1407I 195 TGTACA T^GTACA BspDI 1719 ATCGAT AT^CGAT BsrDI 276 GCAATG GCAATG(2/0) BsrGI 195 TGTACA T^GTACA BssSI 2506 CACGAG CACGAG(-5/-1) BstAPI 1879 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 103 CCATCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 1719 ATCGAT AT^CGAT DrdI 1485 GACGGGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1460 TGGCCA [CT]^GGCC[GA] HincII 736 GTTAAC GT[CT]^[GA]AC HindII 736 GTTAAC GT[CT]^[GA]AC HpaI 736 GTTAAC GTT^AAC MamI 1026 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1460 TGGCCA TGG^CCA MscI 1460 TGGCCA TGG^CCA NcoI 885 CCATGG C^CATGG PmaCI 240 CACGTG CAC^GTG PmlI 240 CACGTG CAC^GTG PstI 1387 CTGCAG CTGCA^G SacI 23 GAGCTC GAGCT^C SmaI 1997 CCCGGG CCC^GGG SphI 1559 GCATGC GCATG^C SspBI 195 TGTACA T^GTACA XmaCI 1997 CCCGGG C^CCGGG XmaI 1997 CCCGGG C^CCGGG XmnI 1914 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1009 GTCTAC GT^[AC][GT]AC 1084 GTAGAC Alw44I 759 GTGCAC G^TGCAC 1191 GTGCAC AocI 1695 CCTGAGG CC^T[ACGT]AGG 2101 CCTCAGG ApaI 499 GGGCCC GGGCC^C 531 GGGCCC ApaLI 759 GTGCAC G^TGCAC 1191 GTGCAC BanI 787 GGCGCC G^G[CT][GA]CC 1612 GGCGCC BbeI 787 GGCGCC GGCGC^C 1612 GGCGCC BlpI 1286 GCTCAGC GC^T[ACGT]AGC 1766 GCTCAGC Bpu1102I 1286 GCTCAGC GC^T[ACGT]AGC 1766 GCTCAGC BspMI 481 ACCTGC ACCTGC(4/8) 1814 ACCTGC BstYI 629 GGATCT [GA]^GATC[CT] 1603 GGATCC Bsu36I 1695 CCTGAGG CC^T[ACGT]AGG 2101 CCTCAGG CelII 1286 GCTCAGC GC^T[ACGT]AGC 1766 GCTCAGC DsaI 885 CCATGG C^C[GA][CT]GG 1552 CCACGG Eco81I 1695 CCTGAGG CC^T[ACGT]AGG 2101 CCTCAGG EcoNI 1575 CCTAGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2301 CCTGTTCCAGG FauI 683 CCCGC CCCGC(4/6) 1211 CCCGC HaeII 787 GGCGCC [GA]GCGC^[CT] 1612 GGCGCC KasI 787 GGCGCC G^GCGCC 1612 GGCGCC MaeII 241 ACGT A^CGT 2275 ACGT MflI 629 GGATCT [GA]^GATC[CT] 1603 GGATCC NarI 787 GGCGCC GG^CGCC 1612 GGCGCC NspI 1214 GCATGT [GA]CATG^[CT] 1559 GCATGC SapI 2423 GAAGAGC GCTCTTC(1/4) 114 GCTCTTC ScaI 1130 AGTACT AGT^ACT 2433 AGTACT SfoI 787 GGCGCC GGC^GCC 1612 GGCGCC SmlI 385 CTCAAG C^T[CT][GA]AG 519 CTCAAG TfiI 575 GATTC G^A[AT]TC 1319 GAATC XbaI 158 TCTAGA T^CTAGA 1786 TCTAGA XhoII 629 GGATCT [GA]^GATC[CT] 1603 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaII AvrII BanII BbsI BbvI BcnI BfaI BlnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI