##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AviII BciVI BclI BfrI BglII
BsaWI BseAI BsiWI BsmBI BsmI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Cfr10I CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcsI 92 AAATTC [GA]^AATT[CT]
AflIII 239 ACACGT A^C[GA][CT]GT
ApoI 92 AAATTC [GA]^AATT[CT]
Asp700I 1914 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1997 CCCGGG C^[CT]CG[GA]G
BalI 1460 TGGCCA TGG^CCA
BamHI 1603 GGATCC G^GATCC
BbrPI 240 CACGTG CAC^GTG
BbvCI 1761 CCTCAGC CCTCAGC(-5/-2)
BcgI 1315 CGAAGAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1701 GCCTAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 240 CACGTG [CT]AC^GT[GA]
BsaBI 1026 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 776 CGGTCG CG[GA][CT]^CG
BsoBI 1997 CCCGGG C^[CT]CG[GA]G
Bsp1407I 195 TGTACA T^GTACA
BspDI 1719 ATCGAT AT^CGAT
BsrDI 276 GCAATG GCAATG(2/0)
BsrGI 195 TGTACA T^GTACA
BssSI 2506 CACGAG CACGAG(-5/-1)
BstAPI 1879 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 103 CCATCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1719 ATCGAT AT^CGAT
DrdI 1485 GACGGGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1460 TGGCCA [CT]^GGCC[GA]
HincII 736 GTTAAC GT[CT]^[GA]AC
HindII 736 GTTAAC GT[CT]^[GA]AC
HpaI 736 GTTAAC GTT^AAC
MamI 1026 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1460 TGGCCA TGG^CCA
MscI 1460 TGGCCA TGG^CCA
NcoI 885 CCATGG C^CATGG
PmaCI 240 CACGTG CAC^GTG
PmlI 240 CACGTG CAC^GTG
PstI 1387 CTGCAG CTGCA^G
SacI 23 GAGCTC GAGCT^C
SmaI 1997 CCCGGG CCC^GGG
SphI 1559 GCATGC GCATG^C
SspBI 195 TGTACA T^GTACA
XmaCI 1997 CCCGGG C^CCGGG
XmaI 1997 CCCGGG C^CCGGG
XmnI 1914 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1009 GTCTAC GT^[AC][GT]AC
1084 GTAGAC
Alw44I 759 GTGCAC G^TGCAC
1191 GTGCAC
AocI 1695 CCTGAGG CC^T[ACGT]AGG
2101 CCTCAGG
ApaI 499 GGGCCC GGGCC^C
531 GGGCCC
ApaLI 759 GTGCAC G^TGCAC
1191 GTGCAC
BanI 787 GGCGCC G^G[CT][GA]CC
1612 GGCGCC
BbeI 787 GGCGCC GGCGC^C
1612 GGCGCC
BlpI 1286 GCTCAGC GC^T[ACGT]AGC
1766 GCTCAGC
Bpu1102I 1286 GCTCAGC GC^T[ACGT]AGC
1766 GCTCAGC
BspMI 481 ACCTGC ACCTGC(4/8)
1814 ACCTGC
BstYI 629 GGATCT [GA]^GATC[CT]
1603 GGATCC
Bsu36I 1695 CCTGAGG CC^T[ACGT]AGG
2101 CCTCAGG
CelII 1286 GCTCAGC GC^T[ACGT]AGC
1766 GCTCAGC
DsaI 885 CCATGG C^C[GA][CT]GG
1552 CCACGG
Eco81I 1695 CCTGAGG CC^T[ACGT]AGG
2101 CCTCAGG
EcoNI 1575 CCTAGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2301 CCTGTTCCAGG
FauI 683 CCCGC CCCGC(4/6)
1211 CCCGC
HaeII 787 GGCGCC [GA]GCGC^[CT]
1612 GGCGCC
KasI 787 GGCGCC G^GCGCC
1612 GGCGCC
MaeII 241 ACGT A^CGT
2275 ACGT
MflI 629 GGATCT [GA]^GATC[CT]
1603 GGATCC
NarI 787 GGCGCC GG^CGCC
1612 GGCGCC
NspI 1214 GCATGT [GA]CATG^[CT]
1559 GCATGC
SapI 2423 GAAGAGC GCTCTTC(1/4)
114 GCTCTTC
ScaI 1130 AGTACT AGT^ACT
2433 AGTACT
SfoI 787 GGCGCC GGC^GCC
1612 GGCGCC
SmlI 385 CTCAAG C^T[CT][GA]AG
519 CTCAAG
TfiI 575 GATTC G^A[AT]TC
1319 GAATC
XbaI 158 TCTAGA T^CTAGA
1786 TCTAGA
XhoII 629 GGATCT [GA]^GATC[CT]
1603 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII AvrII BanII
BbsI BbvI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI