##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI AspI AviII BalI BciVI
BclI BfrI BglII BsaBI BsaI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssSI Bst1107I BstBI BstXI
BstZ17I ClaI Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI MamI MfeI MluI MluNI
MscI MunI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI SacI SalI SapI
ScaI SexAI SfiI SfuI SnaBI
SspBI StuI SwaI Tth111I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 183 GGTACC G^GTACC
AccI 2062 GTCTAC GT^[AC][GT]AC
AccIII 1368 TCCGGA T^CCGGA
Alw44I 2381 GTGCAC G^TGCAC
Aor51HI 263 AGCGCT AGC^GCT
ApaLI 2381 GTGCAC G^TGCAC
Asp718I 183 GGTACC G^GTACC
AvrII 1755 CCTAGG C^CTAGG
BamHI 2538 GGATCC G^GATCC
BbrPI 1192 CACGTG CAC^GTG
BcgI 1050 CGAACGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1755 CCTAGG C^CTAGG
BsaAI 1192 CACGTG [CT]AC^GT[GA]
BseAI 1368 TCCGGA T^CCGGA
BsgI 1582 GTGCAG GTGCAG(16/14)
BsmI 630 GCATTC GAATGC(1/-1)
BspEI 1368 TCCGGA T^CCGGA
BsrDI 480 CATTGC GCAATG(2/0)
BssHII 1143 GCGCGC G^CGCGC
BstAPI 1767 GCAGGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1570 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1424 CGGCCG C^GGCCG
EarI 2695 CTCTTC CTCTTC(1/4)
EclXI 1424 CGGCCG C^GGCCG
Eco47III 263 AGCGCT AGC^GCT
Eco52I 1424 CGGCCG C^GGCCG
HincII 871 GTCAAC GT[CT]^[GA]AC
HindII 871 GTCAAC GT[CT]^[GA]AC
KpnI 183 GGTACC GGTAC^C
Ksp632I 2695 CTCTTC CTCTTC(1/4)
KspI 1362 CCGCGG CCGC^GG
MroI 1368 TCCGGA T^CCGGA
NaeI 1244 GCCGGC GCC^GGC
NgoMIV 1244 GCCGGC G^CCGGC
NotI 1423 GCGGCCGC GC^GGCCGC
PflMI 1569 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1192 CACGTG CAC^GTG
PmlI 1192 CACGTG CAC^GTG
SacII 1362 CCGCGG CCGC^GG
SgrAI 1131 CACCGGCG C[GA]^CCGG[CT]G
SpeI 2727 ACTAGT A^CTAGT
SphI 2599 GCATGC GCATG^C
Van91I 1569 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 1122 GTGCTC G[AT]GC[AT]^C
2381 GTGCAC
BpmI 1702 CTGGAG CTGGAG(16/14)
1939 CTGGAG
BsiEI 1424 CGGCCG CG[GA][CT]^CG
1663 CGACCG
BsiHKAI 1122 GTGCTC G[AT]GC[AT]^C
2381 GTGCAC
BspMI 251 ACCTGC ACCTGC(4/8)
2776 ACCTGC
BsrBI 1785 CCGCTC CCGCTC(-3/-3)
2214 GAGCGG
BsrFI 1132 ACCGGC [GA]^CCGG[CT]
1244 GCCGGC
Cfr10I 1132 ACCGGC [GA]^CCGG[CT]
1244 GCCGGC
CpoI 596 CGGACCG CG^G[AT]CCG
1365 CGGTCCG
DraI 2908 TTTAAA TTT^AAA
2928 TTTAAA
DrdI 274 GACCTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2492 GACCTCTAAGTC
EcoNI 294 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3082 CCTTCCTCAGG
NcoI 1106 CCATGG C^CATGG
2238 CCATGG
RsrII 596 CGGACCG CG^G[AT]CCG
1365 CGGTCCG
SmlI 298 CTCAAG C^T[CT][GA]AG
472 CTCAAG
SspI 25 AATATT AAT^ATT
2861 AATATT
TaqI 362 TCGA T^CGA
716 TCGA
TthHB8I 362 TCGA T^CGA
716 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AvaI AvaII BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI Bpu1102I
BpuAI BsaHI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SfoI SmaI Sse8387I StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmaCI XmaI