################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmI BspEI BspHI BspLU11I BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CpoI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HpaI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspI SwaI TfiI XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AhdI 1676 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 1676 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2310 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 106 TGCGCA TGC^GCA BciVI 426 GTATCC GTATCC(6/5) BsaAI 2239 CACGTA [CT]AC^GT[GA] BsaI 1210 GAGACC GGTCTC(1/5) Bsp1407I 1751 TGTACA T^GTACA BspDI 1770 ATCGAT AT^CGAT BsrBI 978 GAGCGG CCGCTC(-3/-3) BsrFI 1539 GCCGGC [GA]^CCGG[CT] BsrGI 1751 TGTACA T^GTACA BssSI 726 CTCGTG CACGAG(-5/-1) Cfr10I 1539 GCCGGC [GA]^CCGG[CT] ClaI 1770 ATCGAT AT^CGAT DraI 1780 TTTAAA TTT^AAA DrdI 1120 GACCATTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 995 CCACGG C^C[GA][CT]GG EaeI 981 CGGCCA [CT]^GGCC[GA] Eam1105I 1676 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 131 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 2539 CCTTGG C^C[AT][AT]GG FspI 106 TGCGCA TGC^GCA HgaI 2455 GCGTC GACGC(5/10) HindIII 1914 AAGCTT A^AGCTT NaeI 1539 GCCGGC GCC^GGC NgoMIV 1539 GCCGGC G^CCGGC PflFI 2310 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC SapI 1534 GAAGAGC GCTCTTC(1/4) ScaI 713 AGTACT AGT^ACT SmaI 1115 CCCGGG CCC^GGG SspBI 1751 TGTACA T^GTACA StuI 1421 AGGCCT AGG^CCT StyI 2539 CCTTGG C^C[AT][AT]GG TaqI 1771 TCGA T^CGA Tsp509I 2128 AATT ^AATT Tth111I 2310 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1771 TCGA T^CGA XmaCI 1115 CCCGGG C^CCGGG XmaI 1115 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 1062 GGCGCC G[GA]^CG[CT]C 1519 GGCGCC AlwNI 251 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1581 CAGGGACTG AvaI 1115 CCCGGG C^[CT]CG[GA]G 1237 CCCGAG BbeI 1062 GGCGCC GGCGC^C 1519 GGCGCC BpmI 1417 CTCCAG CTGGAG(16/14) 1486 CTCCAG BsaHI 1062 GGCGCC G[GA]^CG[CT]C 1519 GGCGCC BsmBI 2068 CGTCTC CGTCTC(1/5) 1631 GAGACG BsoBI 1115 CCCGGG C^[CT]CG[GA]G 1237 CCCGAG BsrDI 707 CATTGC GCAATG(2/0) 177 GCAATG DraIII 688 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 1613 CACTTGGTG HaeII 1062 GGCGCC [GA]GCGC^[CT] 1519 GGCGCC Hin1I 1062 GGCGCC G[GA]^CG[CT]C 1519 GGCGCC HincII 531 GTCAAC GT[CT]^[GA]AC 1673 GTTGAC HindII 531 GTCAAC GT[CT]^[GA]AC 1673 GTTGAC KasI 1062 GGCGCC G^GCGCC 1519 GGCGCC MaeII 838 ACGT A^CGT 2240 ACGT NarI 1062 GGCGCC GG^CGCC 1519 GGCGCC NspI 271 GCATGT [GA]CATG^[CT] 2403 ACATGC PflMI 579 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2123 CCAGGAATTGG SacI 1929 GAGCTC GAGCT^C 2352 GAGCTC SfoI 1062 GGCGCC GGC^GCC 1519 GGCGCC SpeI 1669 ACTAGT A^CTAGT 1921 ACTAGT Van91I 579 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2123 CCAGGAATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### Acc65I AciI AfaI AluI Asp718I AspHI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI Tru9I TseI Tsp45I TspRI XcmI