##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI BspEI BspHI BspLU11I BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CpoI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HpaI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SphI Sse8387I SspI SwaI
TfiI XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AhdI 1676 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1676 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2310 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 106 TGCGCA TGC^GCA
BciVI 426 GTATCC GTATCC(6/5)
BsaAI 2239 CACGTA [CT]AC^GT[GA]
BsaI 1210 GAGACC GGTCTC(1/5)
Bsp1407I 1751 TGTACA T^GTACA
BspDI 1770 ATCGAT AT^CGAT
BsrBI 978 GAGCGG CCGCTC(-3/-3)
BsrFI 1539 GCCGGC [GA]^CCGG[CT]
BsrGI 1751 TGTACA T^GTACA
BssSI 726 CTCGTG CACGAG(-5/-1)
Cfr10I 1539 GCCGGC [GA]^CCGG[CT]
ClaI 1770 ATCGAT AT^CGAT
DraI 1780 TTTAAA TTT^AAA
DrdI 1120 GACCATTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 995 CCACGG C^C[GA][CT]GG
EaeI 981 CGGCCA [CT]^GGCC[GA]
Eam1105I 1676 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 131 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 2539 CCTTGG C^C[AT][AT]GG
FspI 106 TGCGCA TGC^GCA
HgaI 2455 GCGTC GACGC(5/10)
HindIII 1914 AAGCTT A^AGCTT
NaeI 1539 GCCGGC GCC^GGC
NgoMIV 1539 GCCGGC G^CCGGC
PflFI 2310 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1534 GAAGAGC GCTCTTC(1/4)
ScaI 713 AGTACT AGT^ACT
SmaI 1115 CCCGGG CCC^GGG
SspBI 1751 TGTACA T^GTACA
StuI 1421 AGGCCT AGG^CCT
StyI 2539 CCTTGG C^C[AT][AT]GG
TaqI 1771 TCGA T^CGA
Tsp509I 2128 AATT ^AATT
Tth111I 2310 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1771 TCGA T^CGA
XmaCI 1115 CCCGGG C^CCGGG
XmaI 1115 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 1062 GGCGCC G[GA]^CG[CT]C
1519 GGCGCC
AlwNI 251 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1581 CAGGGACTG
AvaI 1115 CCCGGG C^[CT]CG[GA]G
1237 CCCGAG
BbeI 1062 GGCGCC GGCGC^C
1519 GGCGCC
BpmI 1417 CTCCAG CTGGAG(16/14)
1486 CTCCAG
BsaHI 1062 GGCGCC G[GA]^CG[CT]C
1519 GGCGCC
BsmBI 2068 CGTCTC CGTCTC(1/5)
1631 GAGACG
BsoBI 1115 CCCGGG C^[CT]CG[GA]G
1237 CCCGAG
BsrDI 707 CATTGC GCAATG(2/0)
177 GCAATG
DraIII 688 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1613 CACTTGGTG
HaeII 1062 GGCGCC [GA]GCGC^[CT]
1519 GGCGCC
Hin1I 1062 GGCGCC G[GA]^CG[CT]C
1519 GGCGCC
HincII 531 GTCAAC GT[CT]^[GA]AC
1673 GTTGAC
HindII 531 GTCAAC GT[CT]^[GA]AC
1673 GTTGAC
KasI 1062 GGCGCC G^GCGCC
1519 GGCGCC
MaeII 838 ACGT A^CGT
2240 ACGT
NarI 1062 GGCGCC GG^CGCC
1519 GGCGCC
NspI 271 GCATGT [GA]CATG^[CT]
2403 ACATGC
PflMI 579 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2123 CCAGGAATTGG
SacI 1929 GAGCTC GAGCT^C
2352 GAGCTC
SfoI 1062 GGCGCC GGC^GCC
1519 GGCGCC
SpeI 1669 ACTAGT A^CTAGT
1921 ACTAGT
Van91I 579 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2123 CCAGGAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
Acc65I AciI AfaI AluI Asp718I
AspHI AvaII BanI BanII BbvI
BcnI BfaI BglI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
Tru9I TseI Tsp45I TspRI XcmI