##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI TseI Van91I XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 394 AACGTT AA^CGTT
AlwNI 1519 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 672 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 94 GAGCCC G[GA]GC[CT]^C
BbsI 991 GAAGAC GAAGAC(2/6)
BmyI 94 GAGCCC G[AGT]GC[ACT]^C
BpuAI 991 GAAGAC GAAGAC(2/6)
BsaAI 899 CACGTA [CT]AC^GT[GA]
BseRI 1509 CTCCTC GAGGAG(10/8)
Bsp1286I 94 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 447 TGTACA T^GTACA
BsrBI 606 GAGCGG CCGCTC(-3/-3)
BsrGI 447 TGTACA T^GTACA
BstF5I 511 GGATG GGATG(2/0)
BstXI 1694 CCATCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 630 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 428 TGGCCG [CT]^GGCC[GA]
EcoNI 881 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 630 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 2077 GAATTC G^AATTC
FauI 998 CCCGC CCCGC(4/6)
Fnu4HI 2223 GCCGC GC^[ACGT]GC
FokI 511 GGATG GGATG(9/13)
HincII 219 GTTAAC GT[CT]^[GA]AC
HindII 219 GTTAAC GT[CT]^[GA]AC
HindIII 453 AAGCTT A^AGCTT
HpaI 219 GTTAAC GTT^AAC
ItaI 2223 GCCGC GC^[ACGT]GC
MslI 588 CATAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1683 CAGCTG C[AC]G^C[GT]G
NheI 2219 GCTAGC G^CTAGC
NspI 2215 GCATGC [GA]CATG^[CT]
PflFI 672 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 630 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1077 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 394 AACGTT AA^CGTT
PstI 1341 CTGCAG CTGCA^G
PvuII 1683 CAGCTG CAG^CTG
SexAI 1700 ACCTGGT A^CC[AT]GGT
SphI 2215 GCATGC GCATG^C
SspBI 447 TGTACA T^GTACA
SspI 2680 AATATT AAT^ATT
Tth111I 672 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 770 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BamHI 1875 GGATCC G^GATCC
2138 GGATCC
BsmI 2565 GAATGC GAATGC(1/-1)
2614 GCATTC
BsrDI 592 GCAATG GCAATG(2/0)
1234 GCAATG
BsrFI 430 GCCGGT [GA]^CCGG[CT]
1381 GCCGGT
BssKI 1701 CCTGG ^CC[ACGT]GG
1785 CCTGG
BstNI 1701 CCTGG CC^[AT]GG
1785 CCTGG
Cfr10I 430 GCCGGT [GA]^CCGG[CT]
1381 GCCGGT
EcoRII 1701 CCTGG ^CC[AT]GG
1785 CCTGG
EcoRV 697 GATATC GAT^ATC
1503 GATATC
MaeII 395 ACGT A^CGT
900 ACGT
MvaI 1701 CCTGG CC^[AT]GG
1785 CCTGG
NlaIV 1875 GGATCC GG[ACGT]^[ACGT]CC
2138 GGATCC
ScrFI 1701 CCTGG CC^[ACGT]GG
1785 CCTGG
SmlI 1180 CTCAAG C^T[CT][GA]AG
2707 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BfaI BsaI BsaJI
BsiYI BslI BsmAI BsrI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoT14I HaeIII HapII HinfI HpaII
HphI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
NdeII NlaIII PleI RsaI Sau3AI
Sau96I SfaNI SfcI StyI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I XhoII