################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspHI BspLU11I BspMI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI TseI Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AclI 394 AACGTT AA^CGTT AlwNI 1519 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AspI 672 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 94 GAGCCC G[GA]GC[CT]^C BbsI 991 GAAGAC GAAGAC(2/6) BmyI 94 GAGCCC G[AGT]GC[ACT]^C BpuAI 991 GAAGAC GAAGAC(2/6) BsaAI 899 CACGTA [CT]AC^GT[GA] BseRI 1509 CTCCTC GAGGAG(10/8) Bsp1286I 94 GAGCCC G[AGT]GC[ACT]^C Bsp1407I 447 TGTACA T^GTACA BsrBI 606 GAGCGG CCGCTC(-3/-3) BsrGI 447 TGTACA T^GTACA BstF5I 511 GGATG GGATG(2/0) BstXI 1694 CCATCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 630 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 428 TGGCCG [CT]^GGCC[GA] EcoNI 881 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 630 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 2077 GAATTC G^AATTC FauI 998 CCCGC CCCGC(4/6) Fnu4HI 2223 GCCGC GC^[ACGT]GC FokI 511 GGATG GGATG(9/13) HincII 219 GTTAAC GT[CT]^[GA]AC HindII 219 GTTAAC GT[CT]^[GA]AC HindIII 453 AAGCTT A^AGCTT HpaI 219 GTTAAC GTT^AAC ItaI 2223 GCCGC GC^[ACGT]GC MslI 588 CATAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1683 CAGCTG C[AC]G^C[GT]G NheI 2219 GCTAGC G^CTAGC NspI 2215 GCATGC [GA]CATG^[CT] PflFI 672 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 630 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1077 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 394 AACGTT AA^CGTT PstI 1341 CTGCAG CTGCA^G PvuII 1683 CAGCTG CAG^CTG SexAI 1700 ACCTGGT A^CC[AT]GGT SphI 2215 GCATGC GCATG^C SspBI 447 TGTACA T^GTACA SspI 2680 AATATT AAT^ATT Tth111I 672 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 770 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BamHI 1875 GGATCC G^GATCC 2138 GGATCC BsmI 2565 GAATGC GAATGC(1/-1) 2614 GCATTC BsrDI 592 GCAATG GCAATG(2/0) 1234 GCAATG BsrFI 430 GCCGGT [GA]^CCGG[CT] 1381 GCCGGT BssKI 1701 CCTGG ^CC[ACGT]GG 1785 CCTGG BstNI 1701 CCTGG CC^[AT]GG 1785 CCTGG Cfr10I 430 GCCGGT [GA]^CCGG[CT] 1381 GCCGGT EcoRII 1701 CCTGG ^CC[AT]GG 1785 CCTGG EcoRV 697 GATATC GAT^ATC 1503 GATATC MaeII 395 ACGT A^CGT 900 ACGT MvaI 1701 CCTGG CC^[AT]GG 1785 CCTGG NlaIV 1875 GGATCC GG[ACGT]^[ACGT]CC 2138 GGATCC ScrFI 1701 CCTGG CC^[ACGT]GG 1785 CCTGG SmlI 1180 CTCAAG C^T[CT][GA]AG 2707 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BfaI BsaI BsaJI BsiYI BslI BsmAI BsrI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoT14I HaeIII HapII HinfI HpaII HphI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspI NdeII NlaIII PleI RsaI Sau3AI Sau96I SfaNI SfcI StyI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I XhoII