##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccII AflII AflIII
Alw44I AlwNI ApaI ApaLI AscI
Asp718I AspI AviII AvrII BalI
BamHI BbrPI BclI BfrI BglI
BlnI BsaAI BsaBI BsiEI BsiWI
BsmBI BsmI BspDI BspLU11I BssHII
Bst1107I BstEII BstPI BstUI BstZ17I
ClaI CpoI DraIII EaeI EagI
EclXI Eco52I EcoO65I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MluI
MluNI MscI MvnI NaeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PmaCI PmeI PmlI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SmaI SnaBI SpeI
Sse8387I SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 1347 GTCTAC GT^[AC][GT]AC
AccIII 1732 TCCGGA T^CCGGA
AclI 225 AACGTT AA^CGTT
AgeI 1688 ACCGGT A^CCGGT
AhdI 1839 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 700 GGATC GGATC(4/5)
Aor51HI 2678 AGCGCT AGC^GCT
AspEI 1839 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 743 CTCGGG C^[CT]CG[GA]G
BanII 1603 GAGCTC G[GA]GC[CT]^C
BbeI 716 GGCGCC GGCGC^C
BbsI 1836 GAAGAC GAAGAC(2/6)
BbvCI 779 CCTCAGC CCTCAGC(-5/-2)
BciVI 205 GTATCC GTATCC(6/5)
BcnI 1231 CCCGG CC^[GC]GG
BglII 2119 AGATCT A^GATCT
BlpI 603 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 603 GCTGAGC GC^T[ACGT]AGC
BpuAI 1836 GAAGAC GAAGAC(2/6)
BseAI 1732 TCCGGA T^CCGGA
BseRI 24 GAGGAG GAGGAG(10/8)
BsoBI 743 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2710 TGTACA T^GTACA
BspEI 1732 TCCGGA T^CCGGA
BspHI 1312 TCATGA T^CATGA
BsrBI 144 GAGCGG CCGCTC(-3/-3)
BsrGI 2710 TGTACA T^GTACA
BssSI 21 CACGAG CACGAG(-5/-1)
BstXI 1480 CCATTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2119 AGATCT [GA]^GATC[CT]
CelII 603 GCTGAGC GC^T[ACGT]AGC
DrdI 1494 GACCCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2194 CCATGG C^C[GA][CT]GG
Eam1105I 1839 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2678 AGCGCT AGC^GCT
EcoRI 1509 GAATTC G^AATTC
EcoRV 1997 GATATC GAT^ATC
FauI 146 GCGGG CCCGC(4/6)
KasI 716 GGCGCC G^GCGCC
MfeI 1903 CAATTG C^AATTG
MflI 2119 AGATCT [GA]^GATC[CT]
MroI 1732 TCCGGA T^CCGGA
MunI 1903 CAATTG C^AATTG
NarI 716 GGCGCC GG^CGCC
NciI 1231 CCCGG CC^[GC]GG
NcoI 2194 CCATGG C^CATGG
NdeI 1096 CATATG CA^TATG
PflMI 82 CCATGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1688 ACCGGT A^CCGGT
Psp1406I 225 AACGTT AA^CGTT
RcaI 1312 TCATGA T^CATGA
SacI 1603 GAGCTC GAGCT^C
SapI 1058 GAAGAGC GCTCTTC(1/4)
SfoI 716 GGCGCC GGC^GCC
SgrAI 1687 CACCGGTG C[GA]^CCGG[CT]G
SspBI 2710 TGTACA T^GTACA
Van91I 82 CCATGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 349 CCATTGTGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2119 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 716 GGCGCC G[GA]^CG[CT]C
2190 GACGCC
AocI 815 CCTGAGG CC^T[ACGT]AGG
2332 CCTTAGG
Asp700I 1058 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1509 GAATTCCTTC
BanI 716 GGCGCC G^G[CT][GA]CC
2043 GGTGCC
BcgI 1210 CGAAAGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2381 GCATTGCCTTCG
BpmI 1654 CTCCAG CTGGAG(16/14)
1043 CTGGAG
BsaHI 716 GGCGCC G[GA]^CG[CT]C
2190 GACGCC
BsaI 582 GGTCTC GGTCTC(1/5)
2564 GGTCTC
BsgI 14 GTGCAG GTGCAG(16/14)
1561 GTGCAG
BsrFI 442 ACCGGC [GA]^CCGG[CT]
1688 ACCGGT
BstBI 1208 TTCGAA TT^CGAA
1756 TTCGAA
Bsu36I 815 CCTGAGG CC^T[ACGT]AGG
2332 CCTTAGG
CfoI 717 GCGC GCG^C
2679 GCGC
Cfr10I 442 ACCGGC [GA]^CCGG[CT]
1688 ACCGGT
DraII 748 GGGTCCT [GA]G^G[ACGT]CC[CT]
1492 GGGACCC
Eco81I 815 CCTGAGG CC^T[ACGT]AGG
2332 CCTTAGG
EcoO109I 748 GGGTCCT [GA]G^G[ACGT]CC[CT]
1492 GGGACCC
HaeII 716 GGCGCC [GA]GCGC^[CT]
2678 AGCGCT
HgaI 2190 GACGC GACGC(5/10)
811 GCGTC
HhaI 717 GCGC GCG^C
2679 GCGC
Hin1I 716 GGCGCC G[GA]^CG[CT]C
2190 GACGCC
HinP1I 717 GCGC G^CGC
2679 GCGC
NspV 1208 TTCGAA TT^CGAA
1756 TTCGAA
PpuMI 748 GGGTCCT [GA]G^G[AT]CC[CT]
1492 GGGACCC
PshAI 380 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1461 GACCAGAGTC
PstI 474 CTGCAG CTGCA^G
1842 CTGCAG
ScaI 1930 AGTACT AGT^ACT
2580 AGTACT
SfuI 1208 TTCGAA TT^CGAA
1756 TTCGAA
SmlI 1421 CTCAAG C^T[CT][GA]AG
2430 CTCAAG
SphI 773 GCATGC GCATG^C
2695 GCATGC
SspI 2112 AATATT AAT^ATT
2808 AATATT
StuI 1886 AGGCCT AGG^CCT
2336 AGGCCT
XmnI 1058 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1509 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AseI AsnI AspHI AvaII BbvI
BfaI BmyI BsaJI BsaWI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I