################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflIII AgeI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BglI BlnI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I Cfr10I CpoI DraI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1355 GGTACC G^GTACC AccII 537 CGCG CG^CG AflII 385 CTTAAG C^TTAAG AhdI 605 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 550 CCTGAGG CC^T[ACGT]AGG ApaI 791 GGGCCC GGGCC^C Asp718I 1355 GGTACC G^GTACC AspEI 605 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbvCI 23 GCTGAGG CCTCAGC(-5/-2) BfrI 385 CTTAAG C^TTAAG BglII 263 AGATCT A^GATCT BsaWI 880 TCCGGT [AT]^CCGG[AT] BsmBI 1463 CGTCTC CGTCTC(1/5) Bsp1407I 196 TGTACA T^GTACA BsrGI 196 TGTACA T^GTACA BssSI 35 CTCGTG CACGAG(-5/-1) BstUI 537 CGCG CG^CG BstYI 263 AGATCT [GA]^GATC[CT] Bsu36I 550 CCTGAGG CC^T[ACGT]AGG DraIII 1360 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 260 GACAGATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 605 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 550 CCTGAGG CC^T[ACGT]AGG EcoT22I 69 ATGCAT ATGCA^T KpnI 1355 GGTACC GGTAC^C MaeII 1071 ACGT A^CGT MflI 263 AGATCT [GA]^GATC[CT] MseI 386 TTAA T^TAA MvnI 537 CGCG CG^CG NcoI 244 CCATGG C^CATGG NheI 1302 GCTAGC G^CTAGC NsiI 69 ATGCAT ATGCA^T PaeR7I 93 CTCGAG C^TCGAG PpuMI 630 AGGTCCT [GA]G^G[AT]CC[CT] SexAI 296 ACCAGGT A^CC[AT]GGT SspBI 196 TGTACA T^GTACA Tru9I 386 TTAA T^TAA Tsp509I 1390 AATT ^AATT XcmI 3 CCACAGTTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 93 CTCGAG C^TCGAG XhoII 263 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 412 GTCTAC GT^[AC][GT]AC 1073 GTAGAC AlwNI 404 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1201 CAGCAGCTG AspHI 17 GAGCTC G[AT]GC[AT]^C 865 GAGCTC AvaI 93 CTCGAG C^[CT]CG[GA]G 489 CTCGGG AvaII 631 GGTCC G^G[AT]CC 1177 GGACC BbsI 500 GTCTTC GAAGAC(2/6) 1497 GTCTTC BcnI 728 CCCGG CC^[GC]GG 843 CCCGG BlpI 541 GCTCAGC GC^T[ACGT]AGC 867 GCTCAGC Bpu1102I 541 GCTCAGC GC^T[ACGT]AGC 867 GCTCAGC BpuAI 500 GTCTTC GAAGAC(2/6) 1497 GTCTTC BsgI 461 GTGCAG GTGCAG(16/14) 759 GTGCAG BsiHKAI 17 GAGCTC G[AT]GC[AT]^C 865 GAGCTC BsoBI 93 CTCGAG C^[CT]CG[GA]G 489 CTCGGG BspDI 455 ATCGAT AT^CGAT 1570 ATCGAT BsrDI 799 CATTGC GCAATG(2/0) 1443 CATTGC BstXI 297 CCAGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 781 CCAAGGCCCTGG CelII 541 GCTCAGC GC^T[ACGT]AGC 867 GCTCAGC CfoI 536 GCGC GCG^C 1414 GCGC ClaI 455 ATCGAT AT^CGAT 1570 ATCGAT DpnII 264 GATC ^GATC 580 GATC DsaI 244 CCATGG C^C[GA][CT]GG 529 CCACGG EarI 1031 CTCTTC CTCTTC(1/4) 1227 GAAGAG FauI 693 CCCGC CCCGC(4/6) 916 GCGGG HgaI 638 GACGC GACGC(5/10) 1462 GCGTC HhaI 536 GCGC GCG^C 1414 GCGC HinP1I 536 GCGC G^CGC 1414 GCGC Ksp632I 1031 CTCTTC CTCTTC(1/4) 1227 GAAGAG MboI 264 GATC ^GATC 580 GATC NciI 728 CCCGG CC^[GC]GG 843 CCCGG NdeII 264 GATC ^GATC 580 GATC PvuII 600 CAGCTG CAG^CTG 1204 CAGCTG SacI 17 GAGCTC GAGCT^C 865 GAGCTC Sau3AI 264 GATC ^GATC 580 GATC SfaNI 1378 GCATC GCATC(5/9) 1506 GCATC StuI 934 AGGCCT AGG^CCT 1185 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI BanI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI Cac8I Cfr13I DdeI DraII EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboII MnlI MslI MspA1I MspI MvaI MwoI NlaIII NlaIV NspI PleI RsaI Sau96I ScrFI SmlI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I