##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflIII AgeI Alw44I AlwI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BciVI
BclI BglI BlnI BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsmI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstZ17I Cfr10I CpoI DraI
EaeI EagI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
Van91I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1355 GGTACC G^GTACC
AccII 537 CGCG CG^CG
AflII 385 CTTAAG C^TTAAG
AhdI 605 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 550 CCTGAGG CC^T[ACGT]AGG
ApaI 791 GGGCCC GGGCC^C
Asp718I 1355 GGTACC G^GTACC
AspEI 605 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 23 GCTGAGG CCTCAGC(-5/-2)
BfrI 385 CTTAAG C^TTAAG
BglII 263 AGATCT A^GATCT
BsaWI 880 TCCGGT [AT]^CCGG[AT]
BsmBI 1463 CGTCTC CGTCTC(1/5)
Bsp1407I 196 TGTACA T^GTACA
BsrGI 196 TGTACA T^GTACA
BssSI 35 CTCGTG CACGAG(-5/-1)
BstUI 537 CGCG CG^CG
BstYI 263 AGATCT [GA]^GATC[CT]
Bsu36I 550 CCTGAGG CC^T[ACGT]AGG
DraIII 1360 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 260 GACAGATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 605 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 550 CCTGAGG CC^T[ACGT]AGG
EcoT22I 69 ATGCAT ATGCA^T
KpnI 1355 GGTACC GGTAC^C
MaeII 1071 ACGT A^CGT
MflI 263 AGATCT [GA]^GATC[CT]
MseI 386 TTAA T^TAA
MvnI 537 CGCG CG^CG
NcoI 244 CCATGG C^CATGG
NheI 1302 GCTAGC G^CTAGC
NsiI 69 ATGCAT ATGCA^T
PaeR7I 93 CTCGAG C^TCGAG
PpuMI 630 AGGTCCT [GA]G^G[AT]CC[CT]
SexAI 296 ACCAGGT A^CC[AT]GGT
SspBI 196 TGTACA T^GTACA
Tru9I 386 TTAA T^TAA
Tsp509I 1390 AATT ^AATT
XcmI 3 CCACAGTTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 93 CTCGAG C^TCGAG
XhoII 263 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 412 GTCTAC GT^[AC][GT]AC
1073 GTAGAC
AlwNI 404 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1201 CAGCAGCTG
AspHI 17 GAGCTC G[AT]GC[AT]^C
865 GAGCTC
AvaI 93 CTCGAG C^[CT]CG[GA]G
489 CTCGGG
AvaII 631 GGTCC G^G[AT]CC
1177 GGACC
BbsI 500 GTCTTC GAAGAC(2/6)
1497 GTCTTC
BcnI 728 CCCGG CC^[GC]GG
843 CCCGG
BlpI 541 GCTCAGC GC^T[ACGT]AGC
867 GCTCAGC
Bpu1102I 541 GCTCAGC GC^T[ACGT]AGC
867 GCTCAGC
BpuAI 500 GTCTTC GAAGAC(2/6)
1497 GTCTTC
BsgI 461 GTGCAG GTGCAG(16/14)
759 GTGCAG
BsiHKAI 17 GAGCTC G[AT]GC[AT]^C
865 GAGCTC
BsoBI 93 CTCGAG C^[CT]CG[GA]G
489 CTCGGG
BspDI 455 ATCGAT AT^CGAT
1570 ATCGAT
BsrDI 799 CATTGC GCAATG(2/0)
1443 CATTGC
BstXI 297 CCAGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
781 CCAAGGCCCTGG
CelII 541 GCTCAGC GC^T[ACGT]AGC
867 GCTCAGC
CfoI 536 GCGC GCG^C
1414 GCGC
ClaI 455 ATCGAT AT^CGAT
1570 ATCGAT
DpnII 264 GATC ^GATC
580 GATC
DsaI 244 CCATGG C^C[GA][CT]GG
529 CCACGG
EarI 1031 CTCTTC CTCTTC(1/4)
1227 GAAGAG
FauI 693 CCCGC CCCGC(4/6)
916 GCGGG
HgaI 638 GACGC GACGC(5/10)
1462 GCGTC
HhaI 536 GCGC GCG^C
1414 GCGC
HinP1I 536 GCGC G^CGC
1414 GCGC
Ksp632I 1031 CTCTTC CTCTTC(1/4)
1227 GAAGAG
MboI 264 GATC ^GATC
580 GATC
NciI 728 CCCGG CC^[GC]GG
843 CCCGG
NdeII 264 GATC ^GATC
580 GATC
PvuII 600 CAGCTG CAG^CTG
1204 CAGCTG
SacI 17 GAGCTC GAGCT^C
865 GAGCTC
Sau3AI 264 GATC ^GATC
580 GATC
SfaNI 1378 GCATC GCATC(5/9)
1506 GCATC
StuI 934 AGGCCT AGG^CCT
1185 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI BanI BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstEII BstF5I
BstNI BstPI Cac8I Cfr13I DdeI
DraII EcoO109I EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MwoI NlaIII NlaIV NspI
PleI RsaI Sau96I ScrFI SmlI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I