##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AcyI        
AflIII      AgeI        Alw44I      AlwI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BcgI        BciVI       
BclI        BglI        BlnI        BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BsiEI       BsiWI       
BsmI        BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BssHII      Bst1107I    BstAPI      
BstBI       BstZ17I     Cfr10I      CpoI        DraI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoRI       EcoRV       FbaI        FseI        
FspI        HaeII       Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NotI        NruI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
Van91I      XbaI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       1355  GGTACC               G^GTACC
AccII         537  CGCG                 CG^CG
AflII         385  CTTAAG               C^TTAAG
AhdI          605  GACAGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          550  CCTGAGG              CC^T[ACGT]AGG
ApaI          791  GGGCCC               GGGCC^C
Asp718I      1355  GGTACC               G^GTACC
AspEI         605  GACAGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI          23  GCTGAGG              CCTCAGC(-5/-2)
BfrI          385  CTTAAG               C^TTAAG
BglII         263  AGATCT               A^GATCT
BsaWI         880  TCCGGT               [AT]^CCGG[AT]
BsmBI        1463  CGTCTC               CGTCTC(1/5)
Bsp1407I      196  TGTACA               T^GTACA
BsrGI         196  TGTACA               T^GTACA
BssSI          35  CTCGTG               CACGAG(-5/-1)
BstUI         537  CGCG                 CG^CG
BstYI         263  AGATCT               [GA]^GATC[CT]
Bsu36I        550  CCTGAGG              CC^T[ACGT]AGG
DraIII       1360  CACCTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          260  GACAGATCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      605  GACAGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        550  CCTGAGG              CC^T[ACGT]AGG
EcoT22I        69  ATGCAT               ATGCA^T
KpnI         1355  GGTACC               GGTAC^C
MaeII        1071  ACGT                 A^CGT
MflI          263  AGATCT               [GA]^GATC[CT]
MseI          386  TTAA                 T^TAA
MvnI          537  CGCG                 CG^CG
NcoI          244  CCATGG               C^CATGG
NheI         1302  GCTAGC               G^CTAGC
NsiI           69  ATGCAT               ATGCA^T
PaeR7I         93  CTCGAG               C^TCGAG
PpuMI         630  AGGTCCT              [GA]G^G[AT]CC[CT]
SexAI         296  ACCAGGT              A^CC[AT]GGT
SspBI         196  TGTACA               T^GTACA
Tru9I         386  TTAA                 T^TAA
Tsp509I      1390  AATT                 ^AATT
XcmI            3  CCACAGTTGCTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI           93  CTCGAG               C^TCGAG
XhoII         263  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccI          412  GTCTAC               GT^[AC][GT]AC
             1073  GTAGAC              
AlwNI         404  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1201  CAGCAGCTG           
AspHI          17  GAGCTC               G[AT]GC[AT]^C
              865  GAGCTC              
AvaI           93  CTCGAG               C^[CT]CG[GA]G
              489  CTCGGG              
AvaII         631  GGTCC                G^G[AT]CC
             1177  GGACC               
BbsI          500  GTCTTC               GAAGAC(2/6)
             1497  GTCTTC              
BcnI          728  CCCGG                CC^[GC]GG
              843  CCCGG               
BlpI          541  GCTCAGC              GC^T[ACGT]AGC
              867  GCTCAGC             
Bpu1102I      541  GCTCAGC              GC^T[ACGT]AGC
              867  GCTCAGC             
BpuAI         500  GTCTTC               GAAGAC(2/6)
             1497  GTCTTC              
BsgI          461  GTGCAG               GTGCAG(16/14)
              759  GTGCAG              
BsiHKAI        17  GAGCTC               G[AT]GC[AT]^C
              865  GAGCTC              
BsoBI          93  CTCGAG               C^[CT]CG[GA]G
              489  CTCGGG              
BspDI         455  ATCGAT               AT^CGAT
             1570  ATCGAT              
BsrDI         799  CATTGC               GCAATG(2/0)
             1443  CATTGC              
BstXI         297  CCAGGTTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              781  CCAAGGCCCTGG        
CelII         541  GCTCAGC              GC^T[ACGT]AGC
              867  GCTCAGC             
CfoI          536  GCGC                 GCG^C
             1414  GCGC                
ClaI          455  ATCGAT               AT^CGAT
             1570  ATCGAT              
DpnII         264  GATC                 ^GATC
              580  GATC                
DsaI          244  CCATGG               C^C[GA][CT]GG
              529  CCACGG              
EarI         1031  CTCTTC               CTCTTC(1/4)
             1227  GAAGAG              
FauI          693  CCCGC                CCCGC(4/6)
              916  GCGGG               
HgaI          638  GACGC                GACGC(5/10)
             1462  GCGTC               
HhaI          536  GCGC                 GCG^C
             1414  GCGC                
HinP1I        536  GCGC                 G^CGC
             1414  GCGC                
Ksp632I      1031  CTCTTC               CTCTTC(1/4)
             1227  GAAGAG              
MboI          264  GATC                 ^GATC
              580  GATC                
NciI          728  CCCGG                CC^[GC]GG
              843  CCCGG               
NdeII         264  GATC                 ^GATC
              580  GATC                
PvuII         600  CAGCTG               CAG^CTG
             1204  CAGCTG              
SacI           17  GAGCTC               GAGCT^C
              865  GAGCTC              
Sau3AI        264  GATC                 ^GATC
              580  GATC                
SfaNI        1378  GCATC                GCATC(5/9)
             1506  GCATC               
StuI          934  AGGCCT               AGG^CCT
             1185  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AfaI        AluI        BanI        BanII       
BbvI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       Cac8I       Cfr13I      DdeI        
DraII       EcoO109I    EcoO65I     EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeI        MaeIII      
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NlaIII      NlaIV       NspI        
PleI        RsaI        Sau96I      ScrFI       SmlI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       TthHB8I