##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII BamHI
BanI BanII BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 37 GTCGAC GT^[AC][GT]AC
Asp700I 239 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 107 GTGCTC G[AT]GC[AT]^C
AvrII 392 CCTAGG C^CTAGG
BalI 101 TGGCCA TGG^CCA
BbvCI 692 CCTCAGC CCTCAGC(-5/-2)
BbvI 626 GCAGC GCAGC(8/12)
BcnI 598 CCCGG CC^[GC]GG
BlnI 392 CCTAGG C^CTAGG
BmyI 107 GTGCTC G[AGT]GC[ACT]^C
BsaI 413 GAGACC GGTCTC(1/5)
BsgI 759 GTGCAG GTGCAG(16/14)
BsiHKAI 107 GTGCTC G[AT]GC[AT]^C
BsmAI 413 GAGAC GTCTC(1/5)
Bsp1286I 107 GTGCTC G[AGT]GC[ACT]^C
BspMI 449 GCAGGT ACCTGC(4/8)
BstAPI 535 GCAATCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 783 CCAACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 765 AGATCC [GA]^GATC[CT]
CfoI 608 GCGC GCG^C
EaeI 101 TGGCCA [CT]^GGCC[GA]
EcoRV 279 GATATC GAT^ATC
EcoT14I 392 CCTAGG C^C[AT][AT]GG
FauI 569 GCGGG CCCGC(4/6)
HaeII 607 AGCGCC [GA]GCGC^[CT]
HhaI 608 GCGC GCG^C
HinP1I 608 GCGC G^CGC
HincII 37 GTCGAC GT[CT]^[GA]AC
HindII 37 GTCGAC GT[CT]^[GA]AC
MaeIII 917 GTAAC ^GT[ACGT]AC
MflI 765 AGATCC [GA]^GATC[CT]
MluNI 101 TGGCCA TGG^CCA
MscI 101 TGGCCA TGG^CCA
MslI 869 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 639 CAGCTG C[AC]G^C[GT]G
NciI 598 CCCGG CC^[GC]GG
NheI 362 GCTAGC G^CTAGC
NlaIV 571 GGGACC GG[ACGT]^[ACGT]CC
NspI 406 ACATGC [GA]CATG^[CT]
PshAI 887 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 639 CAGCTG CAG^CTG
SalI 37 GTCGAC G^TCGAC
SexAI 754 ACCTGGT A^CC[AT]GGT
SfaNI 594 GATGC GCATC(5/9)
StuI 580 AGGCCT AGG^CCT
StyI 392 CCTAGG C^C[AT][AT]GG
TseI 626 GCAGC G^C[AT]GC
Tsp509I 89 AATT ^AATT
XhoII 765 AGATCC [GA]^GATC[CT]
XmnI 239 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 202 GCGG CCGC(-3/-1)
569 GCGG
AlwI 501 GATCC GGATC(4/5)
766 GATCC
AocI 228 CCTCAGG CC^T[ACGT]AGG
318 CCTAAGG
AvaII 389 GGTCC G^G[AT]CC
572 GGACC
BfaI 363 CTAG C^TAG
393 CTAG
BpmI 801 CTCCAG CTGGAG(16/14)
721 CTGGAG
BsiYI 344 CCTGGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
563 CCTGTTGCGGG
BslI 344 CCTGGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
563 CCTGTTGCGGG
BsmFI 571 GGGAC GGGAC(10/14)
632 GGGAC
BspHI 181 TCATGA T^CATGA
649 TCATGA
Bsu36I 228 CCTCAGG CC^T[ACGT]AGG
318 CCTAAGG
Cfr13I 389 GGTCC G^G[ACGT]CC
572 GGACC
DpnII 501 GATC ^GATC
766 GATC
Eco81I 228 CCTCAGG CC^T[ACGT]AGG
318 CCTAAGG
Fnu4HI 202 GCGGC GC^[ACGT]GC
626 GCAGC
ItaI 202 GCGGC GC^[ACGT]GC
626 GCAGC
MaeI 363 CTAG C^TAG
393 CTAG
MboI 501 GATC ^GATC
766 GATC
MseI 16 TTAA T^TAA
619 TTAA
MwoI 362 GCTAGCCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
535 GCAATCACTGC
NdeII 501 GATC ^GATC
766 GATC
RcaI 181 TCATGA T^CATGA
649 TCATGA
Sau3AI 501 GATC ^GATC
766 GATC
Sau96I 389 GGTCC G^G[ACGT]CC
572 GGACC
SfcI 127 CTATAG C^T[GA][CT]AG
378 CTACAG
TaqI 38 TCGA T^CGA
247 TCGA
Tru9I 16 TTAA T^TAA
619 TTAA
TthHB8I 38 TCGA T^CGA
247 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI AlwNI BbsI BpuAI
BsaJI BsrI BssKI BstF5I BstNI
Cac8I DdeI EarI EcoRII FokI
HaeIII HapII HinfI HpaII HphI
Ksp632I MboII MnlI MspI MvaI
NlaIII PleI RsaI ScrFI TfiI
TspRI