################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsrII SacI SacII SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 37 GTCGAC GT^[AC][GT]AC Asp700I 239 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 107 GTGCTC G[AT]GC[AT]^C AvrII 392 CCTAGG C^CTAGG BalI 101 TGGCCA TGG^CCA BbvCI 692 CCTCAGC CCTCAGC(-5/-2) BbvI 626 GCAGC GCAGC(8/12) BcnI 598 CCCGG CC^[GC]GG BlnI 392 CCTAGG C^CTAGG BmyI 107 GTGCTC G[AGT]GC[ACT]^C BsaI 413 GAGACC GGTCTC(1/5) BsgI 759 GTGCAG GTGCAG(16/14) BsiHKAI 107 GTGCTC G[AT]GC[AT]^C BsmAI 413 GAGAC GTCTC(1/5) Bsp1286I 107 GTGCTC G[AGT]GC[ACT]^C BspMI 449 GCAGGT ACCTGC(4/8) BstAPI 535 GCAATCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 783 CCAACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 765 AGATCC [GA]^GATC[CT] CfoI 608 GCGC GCG^C EaeI 101 TGGCCA [CT]^GGCC[GA] EcoRV 279 GATATC GAT^ATC EcoT14I 392 CCTAGG C^C[AT][AT]GG FauI 569 GCGGG CCCGC(4/6) HaeII 607 AGCGCC [GA]GCGC^[CT] HhaI 608 GCGC GCG^C HinP1I 608 GCGC G^CGC HincII 37 GTCGAC GT[CT]^[GA]AC HindII 37 GTCGAC GT[CT]^[GA]AC MaeIII 917 GTAAC ^GT[ACGT]AC MflI 765 AGATCC [GA]^GATC[CT] MluNI 101 TGGCCA TGG^CCA MscI 101 TGGCCA TGG^CCA MslI 869 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 639 CAGCTG C[AC]G^C[GT]G NciI 598 CCCGG CC^[GC]GG NheI 362 GCTAGC G^CTAGC NlaIV 571 GGGACC GG[ACGT]^[ACGT]CC NspI 406 ACATGC [GA]CATG^[CT] PshAI 887 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 639 CAGCTG CAG^CTG SalI 37 GTCGAC G^TCGAC SexAI 754 ACCTGGT A^CC[AT]GGT SfaNI 594 GATGC GCATC(5/9) StuI 580 AGGCCT AGG^CCT StyI 392 CCTAGG C^C[AT][AT]GG TseI 626 GCAGC G^C[AT]GC Tsp509I 89 AATT ^AATT XhoII 765 AGATCC [GA]^GATC[CT] XmnI 239 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AciI 202 GCGG CCGC(-3/-1) 569 GCGG AlwI 501 GATCC GGATC(4/5) 766 GATCC AocI 228 CCTCAGG CC^T[ACGT]AGG 318 CCTAAGG AvaII 389 GGTCC G^G[AT]CC 572 GGACC BfaI 363 CTAG C^TAG 393 CTAG BpmI 801 CTCCAG CTGGAG(16/14) 721 CTGGAG BsiYI 344 CCTGGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 563 CCTGTTGCGGG BslI 344 CCTGGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 563 CCTGTTGCGGG BsmFI 571 GGGAC GGGAC(10/14) 632 GGGAC BspHI 181 TCATGA T^CATGA 649 TCATGA Bsu36I 228 CCTCAGG CC^T[ACGT]AGG 318 CCTAAGG Cfr13I 389 GGTCC G^G[ACGT]CC 572 GGACC DpnII 501 GATC ^GATC 766 GATC Eco81I 228 CCTCAGG CC^T[ACGT]AGG 318 CCTAAGG Fnu4HI 202 GCGGC GC^[ACGT]GC 626 GCAGC ItaI 202 GCGGC GC^[ACGT]GC 626 GCAGC MaeI 363 CTAG C^TAG 393 CTAG MboI 501 GATC ^GATC 766 GATC MseI 16 TTAA T^TAA 619 TTAA MwoI 362 GCTAGCCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 535 GCAATCACTGC NdeII 501 GATC ^GATC 766 GATC RcaI 181 TCATGA T^CATGA 649 TCATGA Sau3AI 501 GATC ^GATC 766 GATC Sau96I 389 GGTCC G^G[ACGT]CC 572 GGACC SfcI 127 CTATAG C^T[GA][CT]AG 378 CTACAG TaqI 38 TCGA T^CGA 247 TCGA Tru9I 16 TTAA T^TAA 619 TTAA TthHB8I 38 TCGA T^CGA 247 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AfaI AluI AlwNI BbsI BpuAI BsaJI BsrI BssKI BstF5I BstNI Cac8I DdeI EarI EcoRII FokI HaeIII HapII HinfI HpaII HphI Ksp632I MboII MnlI MspI MvaI NlaIII PleI RsaI ScrFI TfiI TspRI