##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbeI BbrPI BcgI
BclI BfrI BglII BlnI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI TfiI
TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 630 AAATTC [GA]^AATT[CT]
AflIII 653 ACACGT A^C[GA][CT]GT
AhdI 681 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 198 CCTGAGG CC^T[ACGT]AGG
ApaI 1616 GGGCCC GGGCC^C
ApoI 630 AAATTC [GA]^AATT[CT]
AspEI 681 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1488 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 22 GGATAC GTATCC(6/5)
BglI 922 GCCCGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 243 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 243 GCTGAGC GC^T[ACGT]AGC
BsaAI 660 TACGTG [CT]AC^GT[GA]
BsaBI 1624 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1404 GTGCAG GTGCAG(16/14)
BsmBI 238 GAGACG CGTCTC(1/5)
BspHI 74 TCATGA T^CATGA
BsrDI 386 CATTGC GCAATG(2/0)
BstXI 1337 CCACACCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 941 GGATCT [GA]^GATC[CT]
Bsu36I 198 CCTGAGG CC^T[ACGT]AGG
CelII 243 GCTGAGC GC^T[ACGT]AGC
DraI 1668 TTTAAA TTT^AAA
Eam1105I 681 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1678 GAAGAG CTCTTC(1/4)
Eco81I 198 CCTGAGG CC^T[ACGT]AGG
EcoT14I 569 CCATGG C^C[AT][AT]GG
HaeII 669 AGCGCC [GA]GCGC^[CT]
Ksp632I 1678 GAAGAG CTCTTC(1/4)
MamI 1624 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 941 GGATCT [GA]^GATC[CT]
MseI 1669 TTAA T^TAA
NcoI 569 CCATGG C^CATGG
NheI 113 GCTAGC G^CTAGC
NspI 1097 GCATGT [GA]CATG^[CT]
PflFI 1488 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1850 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 74 TCATGA T^CATGA
SacI 1653 GAGCTC GAGCT^C
SbfI 1163 CCTGCAGG CCTGCA^GG
SmaI 937 CCCGGG CCC^GGG
Sse8387I 1163 CCTGCAGG CCTGCA^GG
StyI 569 CCATGG C^C[AT][AT]GG
Tru9I 1669 TTAA T^TAA
Tsp509I 631 AATT ^AATT
Tth111I 1488 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1853 CCAGGTCAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 941 GGATCT [GA]^GATC[CT]
XmaCI 937 CCCGGG C^CCGGG
XmaI 937 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 657 GTCTAC GT^[AC][GT]AC
1760 GTCTAC
AccII 1021 CGCG CG^CG
1257 CGCG
BalI 511 TGGCCA TGG^CCA
532 TGGCCA
BbvCI 1415 GCTGAGG CCTCAGC(-5/-2)
1504 GCTGAGG
BcnI 937 CCCGG CC^[GC]GG
938 CCGGG
BsaI 225 GAGACC GGTCTC(1/5)
47 GGTCTC
BstUI 1021 CGCG CG^CG
1257 CGCG
FauI 923 CCCGC CCCGC(4/6)
860 GCGGG
MaeII 655 ACGT A^CGT
661 ACGT
MluNI 511 TGGCCA TGG^CCA
532 TGGCCA
MscI 511 TGGCCA TGG^CCA
532 TGGCCA
MvnI 1021 CGCG CG^CG
1257 CGCG
NciI 937 CCCGG CC^[GC]GG
938 CCGGG
PpuMI 863 GGGTCCC [GA]G^G[AT]CC[CT]
1158 AGGTCCC
SmlI 745 CTCAAG C^T[CT][GA]AG
753 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EcoNI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I TspRI Van91I XmnI