##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I AspI AviII
BamHI BbeI BbrPI BcgI BfrI
BglII BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI MamI MflI
MluI MroI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
TfiI Tth111I XbaI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1505 GGTACC G^GTACC
AcsI 2137 GAATTT [GA]^AATT[CT]
AcyI 1340 GGCGTC G[GA]^CG[CT]C
AlwNI 1659 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 2137 GAATTT [GA]^AATT[CT]
Asp718I 1505 GGTACC G^GTACC
AvrII 1400 CCTAGG C^CTAGG
BalI 801 TGGCCA TGG^CCA
BbsI 1284 GTCTTC GAAGAC(2/6)
BciVI 312 GGATAC GTATCC(6/5)
BclI 1438 TGATCA T^GATCA
BglI 524 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1400 CCTAGG C^CTAGG
BpuAI 1284 GTCTTC GAAGAC(2/6)
BsaAI 1434 TACGTG [CT]AC^GT[GA]
BsaHI 1340 GGCGTC G[GA]^CG[CT]C
BsmI 1643 GAATGC GAATGC(1/-1)
Bsp1407I 2199 TGTACA T^GTACA
BsrBI 225 GAGCGG CCGCTC(-3/-3)
BsrDI 459 GCAATG GCAATG(2/0)
BsrGI 2199 TGTACA T^GTACA
BstEII 1110 GGTCACC G^GT[ACGT]ACC
BstPI 1110 GGTCACC G^GT[ACGT]ACC
EaeI 801 TGGCCA [CT]^GGCC[GA]
EcoO65I 1110 GGTCACC G^GT[ACGT]ACC
FbaI 1438 TGATCA T^GATCA
Hin1I 1340 GGCGTC G[GA]^CG[CT]C
KpnI 1505 GGTACC GGTAC^C
KspI 1347 CCGCGG CCGC^GG
MfeI 713 CAATTG C^AATTG
MluNI 801 TGGCCA TGG^CCA
MscI 801 TGGCCA TGG^CCA
MunI 713 CAATTG C^AATTG
NspI 813 GCATGT [GA]CATG^[CT]
PflMI 603 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1755 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 1862 GAGCTC GAGCT^C
SacII 1347 CCGCGG CCGC^GG
SfiI 523 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 2199 TGTACA T^GTACA
SspI 170 AATATT AAT^ATT
Van91I 603 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AhdI 1713 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1945 GACCCCACGTC
AlwI 730 GATCC GGATC(4/5)
1786 GATCC
Aor51HI 363 AGCGCT AGC^GCT
1144 AGCGCT
ApaI 522 GGGCCC GGGCC^C
706 GGGCCC
AspEI 1713 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1945 GACCCCACGTC
BlpI 1601 GCTCAGC GC^T[ACGT]AGC
1859 GCTGAGC
Bpu1102I 1601 GCTCAGC GC^T[ACGT]AGC
1859 GCTGAGC
BsgI 1222 CTGCAC GTGCAG(16/14)
1989 CTGCAC
CelII 1601 GCTCAGC GC^T[ACGT]AGC
1859 GCTGAGC
DraIII 1386 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1704 CACCTGGTG
DsaI 969 CCGTGG C^C[GA][CT]GG
1347 CCGCGG
Eam1105I 1713 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1945 GACCCCACGTC
Eco47III 363 AGCGCT AGC^GCT
1144 AGCGCT
EcoNI 74 CCTGAGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1849 CCTCCAACAGG
HgaI 1341 GCGTC GACGC(5/10)
1778 GCGTC
HincII 695 GTCAAC GT[CT]^[GA]AC
764 GTCAAC
HindII 695 GTCAAC GT[CT]^[GA]AC
764 GTCAAC
MaeII 1435 ACGT A^CGT
1951 ACGT
MseI 176 TTAA T^TAA
944 TTAA
PaeR7I 1768 CTCGAG C^TCGAG
1868 CTCGAG
PstI 317 CTGCAG CTGCA^G
1417 CTGCAG
PvuII 734 CAGCTG CAG^CTG
868 CAGCTG
ScaI 621 AGTACT AGT^ACT
666 AGTACT
SexAI 1387 ACCTGGT A^CC[AT]GGT
1705 ACCTGGT
SmaI 1636 CCCGGG CCC^GGG
1752 CCCGGG
SmlI 1768 CTCGAG C^T[CT][GA]AG
1868 CTCGAG
Tru9I 176 TTAA T^TAA
944 TTAA
XcmI 1026 CCAGGGAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1129 CCAGGCTGTTAGTGG
XhoI 1768 CTCGAG C^TCGAG
1868 CTCGAG
XmaCI 1636 CCCGGG C^CCGGG
1752 CCCGGG
XmaI 1636 CCCGGG C^CCGGG
1752 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AocI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I