##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 565 AACGTT AA^CGTT
AcsI 401 AAATTT [GA]^AATT[CT]
AlwNI 843 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 106 CCTGAGG CC^T[ACGT]AGG
ApoI 401 AAATTT [GA]^AATT[CT]
AvaI 464 CCCGAG C^[CT]CG[GA]G
AvaII 924 GGACC G^G[AT]CC
BanI 194 GGTGCC G^G[CT][GA]CC
BanII 46 GAGCCC G[GA]GC[CT]^C
BbsI 485 GAAGAC GAAGAC(2/6)
BglII 279 AGATCT A^GATCT
BpuAI 485 GAAGAC GAAGAC(2/6)
BsaI 892 GGTCTC GGTCTC(1/5)
BseRI 227 CTCCTC GAGGAG(10/8)
BsmFI 372 GTCCC GGGAC(10/14)
BsoBI 464 CCCGAG C^[CT]CG[GA]G
Bsp1407I 92 TGTACA T^GTACA
BsrBI 863 GAGCGG CCGCTC(-3/-3)
BsrDI 98 GCAATG GCAATG(2/0)
BsrGI 92 TGTACA T^GTACA
BstXI 659 CCAGCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 106 CCTGAGG CC^T[ACGT]AGG
CfoI 855 GCGC GCG^C
DraI 570 TTTAAA TTT^AAA
DsaI 312 CCATGG C^C[GA][CT]GG
Eco81I 106 CCTGAGG CC^T[ACGT]AGG
EcoT14I 312 CCATGG C^C[AT][AT]GG
FauI 1081 CCCGC CCCGC(4/6)
HhaI 855 GCGC GCG^C
HinP1I 855 GCGC G^CGC
MspA1I 846 CAGCTG C[AC]G^C[GT]G
NcoI 312 CCATGG C^CATGG
NheI 935 GCTAGC G^CTAGC
PflMI 1216 CCAGACGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 565 AACGTT AA^CGTT
PstI 82 CTGCAG CTGCA^G
PvuII 846 CAGCTG CAG^CTG
SapI 515 GAAGAGC GCTCTTC(1/4)
SfaNI 932 GATGC GCATC(5/9)
SmlI 449 CTCAAG C^T[CT][GA]AG
SspBI 92 TGTACA T^GTACA
StyI 312 CCATGG C^C[AT][AT]GG
Tsp45I 871 GTGAC ^GT[GC]AC
Tsp509I 402 AATT ^AATT
Van91I 1216 CCAGACGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 387 GATCC GGATC(4/5)
1213 GATCC
BalI 284 TGGCCA TGG^CCA
1174 TGGCCA
BcnI 375 CCCGG CC^[GC]GG
875 CCCGG
BmyI 46 GAGCCC G[AGT]GC[ACT]^C
994 GTGCCC
BsiYI 858 CCTCAGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1216 CCAGACGTTGG
BslI 858 CCTCAGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1216 CCAGACGTTGG
Bsp1286I 46 GAGCCC G[AGT]GC[ACT]^C
994 GTGCCC
BstYI 279 AGATCT [GA]^GATC[CT]
386 AGATCC
Cfr13I 924 GGACC G^G[ACGT]CC
1096 GGCCC
EarI 515 GAAGAG CTCTTC(1/4)
547 GAAGAG
Ksp632I 515 GAAGAG CTCTTC(1/4)
547 GAAGAG
MflI 279 AGATCT [GA]^GATC[CT]
386 AGATCC
MluNI 284 TGGCCA TGG^CCA
1174 TGGCCA
MscI 284 TGGCCA TGG^CCA
1174 TGGCCA
NciI 375 CCCGG CC^[GC]GG
875 CCCGG
Sau96I 924 GGACC G^G[ACGT]CC
1096 GGCCC
SfcI 82 CTGCAG C^T[GA][CT]AG
275 CTATAG
XhoII 279 AGATCT [GA]^GATC[CT]
386 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI BbvCI BbvI
BfaI BpmI BsaJI BsmAI BsrI
BssKI BstNI Cac8I DdeI DpnII
EaeI EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI ScrFI TfiI Tru9I
TseI TspRI