##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AviII BbeI BclI BfrI BglI
BglII BsaHI BseAI BsiWI BsmBI
BspEI BspHI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CpoI
DraI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
MfeI MluI MroI MunI NarI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1291 GTCTAC GT^[AC][GT]AC
AcsI 3960 AAATTC [GA]^AATT[CT]
AhdI 1631 GACCGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3960 AAATTC [GA]^AATT[CT]
Asp700I 1056 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1631 GACCGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3881 CACGTG CAC^GTG
BcgI 1646 GCAGCTAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 3881 CACGTG [CT]AC^GT[GA]
BsaBI 591 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 1105 ATCGAT AT^CGAT
BspMI 1487 GCAGGT ACCTGC(4/8)
BsrDI 2785 CATTGC GCAATG(2/0)
BsrFI 1186 GCCGGC [GA]^CCGG[CT]
BssSI 3680 CTCGTG CACGAG(-5/-1)
Cfr10I 1186 GCCGGC [GA]^CCGG[CT]
ClaI 1105 ATCGAT AT^CGAT
Eam1105I 1631 GACCGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 3239 GTCAAC GT[CT]^[GA]AC
HindII 3239 GTCAAC GT[CT]^[GA]AC
MamI 591 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1186 GCCGGC GCC^GGC
NdeI 2611 CATATG CA^TATG
NgoMIV 1186 GCCGGC G^CCGGC
PaeR7I 27 CTCGAG C^TCGAG
PmaCI 3881 CACGTG CAC^GTG
PmlI 3881 CACGTG CAC^GTG
PshAI 1556 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 3832 ACCAGGT A^CC[AT]GGT
SmaI 165 CCCGGG CCC^GGG
SpeI 2730 ACTAGT A^CTAGT
SphI 572 GCATGC GCATG^C
SspI 524 AATATT AAT^ATT
XbaI 1712 TCTAGA T^CTAGA
XhoI 27 CTCGAG C^TCGAG
XmaCI 165 CCCGGG C^CCGGG
XmaI 165 CCCGGG C^CCGGG
XmnI 1056 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 100 CGCG CG^CG
146 CGCG
AvaI 27 CTCGAG C^[CT]CG[GA]G
165 CCCGGG
BciVI 1030 GTATCC GTATCC(6/5)
3255 GTATCC
BlpI 641 GCTCAGC GC^T[ACGT]AGC
2459 GCTCAGC
Bpu1102I 641 GCTCAGC GC^T[ACGT]AGC
2459 GCTCAGC
BsiEI 11 CGGCCG CG[GA][CT]^CG
96 CGGCCG
BsoBI 27 CTCGAG C^[CT]CG[GA]G
165 CCCGGG
Bsp1407I 680 TGTACA T^GTACA
2495 TGTACA
BspLU11I 551 ACATGT A^CATGT
1508 ACATGT
BsrGI 680 TGTACA T^GTACA
2495 TGTACA
BstUI 100 CGCG CG^CG
146 CGCG
CelII 641 GCTCAGC GC^T[ACGT]AGC
2459 GCTCAGC
DraIII 2191 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3213 CACCCTGTG
EagI 11 CGGCCG C^GGCCG
96 CGGCCG
EclXI 11 CGGCCG C^GGCCG
96 CGGCCG
Eco52I 11 CGGCCG C^GGCCG
96 CGGCCG
HindIII 1890 AAGCTT A^AGCTT
3774 AAGCTT
KspI 99 CCGCGG CCGC^GG
145 CCGCGG
MvnI 100 CGCG CG^CG
146 CGCG
NcoI 172 CCATGG C^CATGG
3865 CCATGG
SacII 99 CCGCGG CCGC^GG
145 CCGCGG
SspBI 680 TGTACA T^GTACA
2495 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI ApaI AspHI AvaII
AvrII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlnI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII