##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BcnI BglI BglII BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaII
KasI KpnI KspI MfeI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AciI 1885 GCGG CCGC(-3/-1)
AlwNI 646 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 234 GAGCAC G[AT]GC[AT]^C
AvaI 1249 CTCGGG C^[CT]CG[GA]G
BalI 1217 TGGCCA TGG^CCA
BbsI 1872 GTCTTC GAAGAC(2/6)
BbvI 31 GCAGC GCAGC(8/12)
BciVI 58 GGATAC GTATCC(6/5)
BpuAI 1872 GTCTTC GAAGAC(2/6)
BsaBI 46 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 223 GAGACC GGTCTC(1/5)
BseRI 1907 GAGGAG GAGGAG(10/8)
BsiHKAI 234 GAGCAC G[AT]GC[AT]^C
BsoBI 1249 CTCGGG C^[CT]CG[GA]G
BspMI 226 ACCTGC ACCTGC(4/8)
BstAPI 533 GCAAATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 470 GGATCT [GA]^GATC[CT]
DsaI 1220 CCATGG C^C[GA][CT]GG
EaeI 1217 TGGCCA [CT]^GGCC[GA]
EarI 1786 GAAGAG CTCTTC(1/4)
EcoRV 1298 GATATC GAT^ATC
Fnu4HI 31 GCAGC GC^[ACGT]GC
ItaI 31 GCAGC GC^[ACGT]GC
Ksp632I 1786 GAAGAG CTCTTC(1/4)
MamI 46 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 470 GGATCT [GA]^GATC[CT]
MluNI 1217 TGGCCA TGG^CCA
MscI 1217 TGGCCA TGG^CCA
NcoI 1220 CCATGG C^CATGG
NdeI 1496 CATATG CA^TATG
NheI 305 GCTAGC G^CTAGC
PpuMI 2845 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 228 CTGCAG CTGCA^G
SbfI 227 CCTGCAGG CCTGCA^GG
Sse8387I 227 CCTGCAGG CCTGCA^GG
TaqI 546 TCGA T^CGA
TseI 31 GCAGC G^C[AT]GC
TthHB8I 546 TCGA T^CGA
XcmI 748 CCAGGAAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 470 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflII 173 CTTAAG C^TTAAG
3136 CTTAAG
AlwI 1980 GATCC GGATC(4/5)
470 GGATC
ApaI 37 GGGCCC GGGCC^C
2856 GGGCCC
AvaII 1964 GGTCC G^G[AT]CC
2846 GGTCC
AvrII 773 CCTAGG C^CTAGG
845 CCTAGG
BanII 37 GGGCCC G[GA]GC[CT]^C
2856 GGGCCC
BfrI 173 CTTAAG C^TTAAG
3136 CTTAAG
BlnI 773 CCTAGG C^CTAGG
845 CCTAGG
BpmI 1902 CTCCAG CTGGAG(16/14)
1629 CTGGAG
BsgI 540 GTGCAG GTGCAG(16/14)
2948 GTGCAG
BsiYI 2046 CCCTTGGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2232 CCATTTTCAGG
BslI 2046 CCCTTGGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2232 CCATTTTCAGG
BsmFI 1503 GGGAC GGGAC(10/14)
2754 GGGAC
BsmI 1841 GAATGC GAATGC(1/-1)
2102 GAATGC
BspLU11I 2211 ACATGT A^CATGT
2494 ACATGT
BssKI 467 CCAGG ^CC[ACGT]GG
748 CCAGG
BstNI 467 CCAGG CC^[AT]GG
748 CCAGG
EcoRII 467 CCAGG ^CC[AT]GG
748 CCAGG
HindIII 1403 AAGCTT A^AGCTT
3139 AAGCTT
HpaI 1435 GTTAAC GTT^AAC
2158 GTTAAC
HphI 1634 GGTGA GGTGA(8/7)
2653 GGTGA
MvaI 467 CCAGG CC^[AT]GG
748 CCAGG
MwoI 370 GCAGAAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
533 GCAAATTGTGC
PleI 2310 GACTC GAGTC(4/5)
3018 GAGTC
ScrFI 467 CCAGG CC^[ACGT]GG
748 CCAGG
SmlI 173 CTTAAG C^T[CT][GA]AG
3136 CTTAAG
SphI 461 GCATGC GCATG^C
2634 GCATGC
SspI 2142 AATATT AAT^ATT
2974 AATATT
TfiI 617 GATTC G^A[AT]TC
788 GATTC
Tsp45I 961 GTCAC ^GT[GC]AC
1879 GTCAC
XbaI 389 TCTAGA T^CTAGA
638 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
AseI AsnI Asp700I BfaI BmyI
BsaJI BsmAI Bsp1286I Bsp1407I BsrGI
BsrI BstF5I Cac8I Cfr13I DdeI
DpnII DraI DraII EcoO109I EcoT14I
EcoT22I FokI HaeIII HincII HindII
HinfI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI NdeII
NlaIII NlaIV NsiI NspI PshBI
RsaI Sau3AI Sau96I SfaNI SfcI
SspBI StyI Tru9I Tsp509I TspRI
XmnI