##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AvaI AviII
AvrII BbeI BbrPI BcgI BfrI
BglI BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
FseI FspI Hin1I HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SapI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 964 GTCGAC GT^[AC][GT]AC
BalI 2678 TGGCCA TGG^CCA
BamHI 3716 GGATCC G^GATCC
BanI 2900 GGCACC G^G[CT][GA]CC
BbvCI 868 CCTCAGC CCTCAGC(-5/-2)
BciVI 2475 GTATCC GTATCC(6/5)
BclI 2130 TGATCA T^GATCA
BcnI 4 CCCGG CC^[GC]GG
BlpI 22 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 22 GCTGAGC GC^T[ACGT]AGC
BsaAI 4008 CACGTA [CT]AC^GT[GA]
BsaBI 633 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 3576 TCATGA T^CATGA
BspMI 3213 GCAGGT ACCTGC(4/8)
BsrBI 273 GAGCGG CCGCTC(-3/-3)
BsrDI 3337 CATTGC GCAATG(2/0)
BstBI 251 TTCGAA TT^CGAA
CelII 22 GCTGAGC GC^T[ACGT]AGC
DraIII 72 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 44 GACGGGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1795 CCGTGG C^C[GA][CT]GG
EarI 1605 GAAGAG CTCTTC(1/4)
EcoT14I 2757 CCTTGG C^C[AT][AT]GG
FbaI 2130 TGATCA T^GATCA
HaeII 33 AGCGCC [GA]GCGC^[CT]
HapII 5 CCGG C^CGG
HgaI 1011 GCGTC GACGC(5/10)
HpaII 5 CCGG C^CGG
Ksp632I 1605 GAAGAG CTCTTC(1/4)
MamI 633 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2678 TGGCCA TGG^CCA
MscI 2678 TGGCCA TGG^CCA
MspI 5 CCGG C^CGG
NciI 4 CCCGG CC^[GC]GG
NdeI 1753 CATATG CA^TATG
NspV 251 TTCGAA TT^CGAA
RcaI 3576 TCATGA T^CATGA
SalI 964 GTCGAC G^TCGAC
SexAI 73 ACCTGGT A^CC[AT]GGT
SfuI 251 TTCGAA TT^CGAA
StyI 2757 CCTTGG C^C[AT][AT]GG
XcmI 2438 CCAGAAGATAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 1177 ACATGT A^C[GA][CT]GT
3912 ACATGT
AhdI 1694 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3935 GACCAATAGTC
AlwNI 3951 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
3990 CAGAATCTG
AspEI 1694 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3935 GACCAATAGTC
BanII 950 GAGCTC G[GA]GC[CT]^C
1055 GAGCTC
BglII 284 AGATCT A^GATCT
2202 AGATCT
BsgI 2285 CTGCAC GTGCAG(16/14)
17 GTGCAG
BspLU11I 1177 ACATGT A^CATGT
3912 ACATGT
CfoI 34 GCGC GCG^C
3444 GCGC
DraII 2723 AGGACCC [GA]G^G[ACGT]CC[CT]
3551 GGGTCCC
EaeI 2550 TGGCCG [CT]^GGCC[GA]
2678 TGGCCA
Eam1105I 1694 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3935 GACCAATAGTC
EcoO109I 2723 AGGACCC [GA]G^G[ACGT]CC[CT]
3551 GGGTCCC
HhaI 34 GCGC GCG^C
3444 GCGC
HinP1I 34 GCGC G^CGC
3444 GCGC
PflMI 155 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
705 CCAGCAGTTGG
PpuMI 2723 AGGACCC [GA]G^G[AT]CC[CT]
3551 GGGTCCC
PstI 204 CTGCAG CTGCA^G
3486 CTGCAG
PvuII 20 CAGCTG CAG^CTG
2827 CAGCTG
SacI 950 GAGCTC GAGCT^C
1055 GAGCTC
ScaI 753 AGTACT AGT^ACT
2685 AGTACT
SphI 808 GCATGC GCATG^C
3440 GCATGC
Van91I 155 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
705 CCAGCAGTTGG
XbaI 617 TCTAGA T^CTAGA
3189 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRI EcoRII EcoT22I FauI Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI