################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspLU11I BsrBI BssHII BssSI BstAPI BstBI BstUI Bsu36I CelII ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoRV FauI FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Aor51HI 1586 AGCGCT AGC^GCT Asp700I 291 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 1126 GAGCTC G[GA]GC[CT]^C BbrPI 1290 CACGTG CAC^GTG BbsI 1237 GTCTTC GAAGAC(2/6) BclI 1372 TGATCA T^GATCA BglII 667 AGATCT A^GATCT BpuAI 1237 GTCTTC GAAGAC(2/6) BsaAI 1290 CACGTG [CT]AC^GT[GA] Bsp1407I 2304 TGTACA T^GTACA BspHI 1157 TCATGA T^CATGA BsrDI 1198 GCAATG GCAATG(2/0) BsrFI 978 ACCGGC [GA]^CCGG[CT] BsrGI 2304 TGTACA T^GTACA Bst1107I 2049 GTATAC GTA^TAC BstXI 523 CCAGAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2049 GTATAC GTA^TAC Cfr10I 978 ACCGGC [GA]^CCGG[CT] EarI 1850 GAAGAG CTCTTC(1/4) Eco47III 1586 AGCGCT AGC^GCT EcoT22I 827 ATGCAT ATGCA^T FbaI 1372 TGATCA T^GATCA HaeII 1586 AGCGCT [GA]GCGC^[CT] HapII 979 CCGG C^CGG HincII 2408 GTTGAC GT[CT]^[GA]AC HindII 2408 GTTGAC GT[CT]^[GA]AC HpaII 979 CCGG C^CGG Ksp632I 1850 GAAGAG CTCTTC(1/4) MaeII 1291 ACGT A^CGT MspI 979 CCGG C^CGG NcoI 1471 CCATGG C^CATGG NdeI 2180 CATATG CA^TATG NsiI 827 ATGCAT ATGCA^T PmaCI 1290 CACGTG CAC^GTG PmlI 1290 CACGTG CAC^GTG RcaI 1157 TCATGA T^CATGA SacI 1126 GAGCTC GAGCT^C SbfI 447 CCTGCAGG CCTGCA^GG SmlI 76 CTCAAG C^T[CT][GA]AG Sse8387I 447 CCTGCAGG CCTGCA^GG SspBI 2304 TGTACA T^GTACA SspI 2078 AATATT AAT^ATT XbaI 1481 TCTAGA T^CTAGA XcmI 2229 CCACTCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 291 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanI 943 GGTGCC G^G[CT][GA]CC 1133 GGTGCC BpmI 800 CTCCAG CTGGAG(16/14) 1003 CTCCAG BseRI 27 CTCCTC GAGGAG(10/8) 1685 GAGGAG BstEII 198 GGTCACC G^GT[ACGT]ACC 990 GGTGACC BstPI 198 GGTCACC G^GT[ACGT]ACC 990 GGTGACC CfoI 1587 GCGC GCG^C 1766 GCGC DraI 1824 TTTAAA TTT^AAA 2742 TTTAAA DsaI 1471 CCATGG C^C[GA][CT]GG 2015 CCGTGG EcoO65I 198 GGTCACC G^GT[ACGT]ACC 990 GGTGACC EcoRI 239 GAATTC G^AATTC 2843 GAATTC EcoT14I 1208 CCAAGG C^C[AT][AT]GG 1471 CCATGG HhaI 1587 GCGC GCG^C 1766 GCGC HinP1I 1587 GCGC G^CGC 1766 GCGC HindIII 2442 AAGCTT A^AGCTT 2769 AAGCTT MslI 101 CACGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1436 CATCATTGTG PflMI 1110 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1619 CCAGCAGGTGG StyI 1208 CCAAGG C^C[AT][AT]GG 1471 CCATGG Van91I 1110 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1619 CCAGCAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BalI BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII