##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BsoBI BspDI BspEI BspLU11I BsrBI
BssHII BssSI BstAPI BstBI BstUI
Bsu36I CelII ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRV
FauI FseI FspI HgaI Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI StuI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Aor51HI 1586 AGCGCT AGC^GCT
Asp700I 291 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 1126 GAGCTC G[GA]GC[CT]^C
BbrPI 1290 CACGTG CAC^GTG
BbsI 1237 GTCTTC GAAGAC(2/6)
BclI 1372 TGATCA T^GATCA
BglII 667 AGATCT A^GATCT
BpuAI 1237 GTCTTC GAAGAC(2/6)
BsaAI 1290 CACGTG [CT]AC^GT[GA]
Bsp1407I 2304 TGTACA T^GTACA
BspHI 1157 TCATGA T^CATGA
BsrDI 1198 GCAATG GCAATG(2/0)
BsrFI 978 ACCGGC [GA]^CCGG[CT]
BsrGI 2304 TGTACA T^GTACA
Bst1107I 2049 GTATAC GTA^TAC
BstXI 523 CCAGAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2049 GTATAC GTA^TAC
Cfr10I 978 ACCGGC [GA]^CCGG[CT]
EarI 1850 GAAGAG CTCTTC(1/4)
Eco47III 1586 AGCGCT AGC^GCT
EcoT22I 827 ATGCAT ATGCA^T
FbaI 1372 TGATCA T^GATCA
HaeII 1586 AGCGCT [GA]GCGC^[CT]
HapII 979 CCGG C^CGG
HincII 2408 GTTGAC GT[CT]^[GA]AC
HindII 2408 GTTGAC GT[CT]^[GA]AC
HpaII 979 CCGG C^CGG
Ksp632I 1850 GAAGAG CTCTTC(1/4)
MaeII 1291 ACGT A^CGT
MspI 979 CCGG C^CGG
NcoI 1471 CCATGG C^CATGG
NdeI 2180 CATATG CA^TATG
NsiI 827 ATGCAT ATGCA^T
PmaCI 1290 CACGTG CAC^GTG
PmlI 1290 CACGTG CAC^GTG
RcaI 1157 TCATGA T^CATGA
SacI 1126 GAGCTC GAGCT^C
SbfI 447 CCTGCAGG CCTGCA^GG
SmlI 76 CTCAAG C^T[CT][GA]AG
Sse8387I 447 CCTGCAGG CCTGCA^GG
SspBI 2304 TGTACA T^GTACA
SspI 2078 AATATT AAT^ATT
XbaI 1481 TCTAGA T^CTAGA
XcmI 2229 CCACTCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 291 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanI 943 GGTGCC G^G[CT][GA]CC
1133 GGTGCC
BpmI 800 CTCCAG CTGGAG(16/14)
1003 CTCCAG
BseRI 27 CTCCTC GAGGAG(10/8)
1685 GAGGAG
BstEII 198 GGTCACC G^GT[ACGT]ACC
990 GGTGACC
BstPI 198 GGTCACC G^GT[ACGT]ACC
990 GGTGACC
CfoI 1587 GCGC GCG^C
1766 GCGC
DraI 1824 TTTAAA TTT^AAA
2742 TTTAAA
DsaI 1471 CCATGG C^C[GA][CT]GG
2015 CCGTGG
EcoO65I 198 GGTCACC G^GT[ACGT]ACC
990 GGTGACC
EcoRI 239 GAATTC G^AATTC
2843 GAATTC
EcoT14I 1208 CCAAGG C^C[AT][AT]GG
1471 CCATGG
HhaI 1587 GCGC GCG^C
1766 GCGC
HinP1I 1587 GCGC G^CGC
1766 GCGC
HindIII 2442 AAGCTT A^AGCTT
2769 AAGCTT
MslI 101 CACGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1436 CATCATTGTG
PflMI 1110 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1619 CCAGCAGGTGG
StyI 1208 CCAAGG C^C[AT][AT]GG
1471 CCATGG
Van91I 1110 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1619 CCAGCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BalI
BbvI BfaI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII