################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII AccII AccIII AflII AgeI AlwI AscI AviII AvrII BamHI BbeI BbrPI BclI BfrI BglI BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I ClaI CpoI DraIII EagI EclXI Eco52I EcoNI EcoT22I FbaI FspI HpaI KasI KspI MamI MluI MroI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SpeI Sse8387I SwaI Van91I XhoI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### Acc65I 262 GGTACC G^GTACC AccI 985 GTCTAC GT^[AC][GT]AC AclI 1753 AACGTT AA^CGTT AcyI 1159 GACGCC G[GA]^CG[CT]C AhdI 2397 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1924 GTGCAC G^TGCAC AocI 181 CCTCAGG CC^T[ACGT]AGG Aor51HI 953 AGCGCT AGC^GCT ApaLI 1924 GTGCAC G^TGCAC AseI 2254 ATTAAT AT^TAAT AsnI 2254 ATTAAT AT^TAAT Asp718I 262 GGTACC G^GTACC AspEI 2397 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2925 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2907 TGGCCA TGG^CCA BanI 262 GGTACC G^G[CT][GA]CC BbvCI 1394 GCTGAGG CCTCAGC(-5/-2) BcgI 1360 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2838 GTATCC GTATCC(6/5) BsaAI 1758 TACGTA [CT]AC^GT[GA] BsaHI 1159 GACGCC G[GA]^CG[CT]C Bsp1407I 2776 TGTACA T^GTACA BspHI 1464 TCATGA T^CATGA BsrFI 1343 GCCGGC [GA]^CCGG[CT] BsrGI 2776 TGTACA T^GTACA BssSI 823 CACGAG CACGAG(-5/-1) BstEII 107 GGTGACC G^GT[ACGT]ACC BstPI 107 GGTGACC G^GT[ACGT]ACC BstXI 1688 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 181 CCTCAGG CC^T[ACGT]AGG Cfr10I 1343 GCCGGC [GA]^CCGG[CT] DrdI 1317 GACTGACGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2397 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 953 AGCGCT AGC^GCT Eco81I 181 CCTCAGG CC^T[ACGT]AGG EcoO65I 107 GGTGACC G^GT[ACGT]ACC EcoRI 2184 GAATTC G^AATTC EcoRV 3205 GATATC GAT^ATC FseI 1342 GGCCGGCC GGCCGG^CC HaeII 953 AGCGCT [GA]GCGC^[CT] HgaI 1159 GACGC GACGC(5/10) Hin1I 1159 GACGCC G[GA]^CG[CT]C HincII 3165 GTTGAC GT[CT]^[GA]AC HindII 3165 GTTGAC GT[CT]^[GA]AC KpnI 262 GGTACC GGTAC^C MfeI 3342 CAATTG C^AATTG MluNI 2907 TGGCCA TGG^CCA MscI 2907 TGGCCA TGG^CCA MunI 3342 CAATTG C^AATTG NaeI 1343 GCCGGC GCC^GGC NcoI 498 CCATGG C^CATGG NgoMIV 1343 GCCGGC G^CCGGC PflFI 2925 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2565 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2254 ATTAAT AT^TAAT Psp1406I 1753 AACGTT AA^CGTT RcaI 1464 TCATGA T^CATGA SapI 577 GAAGAGC GCTCTTC(1/4) ScaI 1724 AGTACT AGT^ACT SmaI 169 CCCGGG CCC^GGG SnaBI 1758 TACGTA TAC^GTA SphI 1191 GCATGC GCATG^C SspBI 2776 TGTACA T^GTACA TfiI 3019 GAATC G^A[AT]TC Tth111I 2925 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 442 TCTAGA T^CTAGA XmaCI 169 CCCGGG C^CCGGG XmaI 169 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 1716 GAATTT [GA]^AATT[CT] 2184 GAATTC AflIII 1912 ACATGT A^C[GA][CT]GT 3065 ACATGT ApoI 1716 GAATTT [GA]^AATT[CT] 2184 GAATTC Asp700I 2184 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2340 GAAGCTTTTC AvaI 169 CCCGGG C^[CT]CG[GA]G 396 CCCGAG BbsI 716 GAAGAC GAAGAC(2/6) 776 GAAGAC BglII 740 AGATCT A^GATCT 1007 AGATCT BlpI 1253 GCTGAGC GC^T[ACGT]AGC 1972 GCTAAGC BpmI 162 CTCCAG CTGGAG(16/14) 2194 CTGGAG Bpu1102I 1253 GCTGAGC GC^T[ACGT]AGC 1972 GCTAAGC BpuAI 716 GAAGAC GAAGAC(2/6) 776 GAAGAC BsaI 1563 GGTCTC GGTCTC(1/5) 1578 GGTCTC BsgI 120 GTGCAG GTGCAG(16/14) 2020 GTGCAG BsoBI 169 CCCGGG C^[CT]CG[GA]G 396 CCCGAG BspLU11I 1912 ACATGT A^CATGT 3065 ACATGT BspMI 1249 ACCTGC ACCTGC(4/8) 1182 GCAGGT BsrI 1876 ACTGG ACTGG(1/-1) 2871 CCAGT BstYI 740 AGATCT [GA]^GATC[CT] 1007 AGATCT CelII 1253 GCTGAGC GC^T[ACGT]AGC 1972 GCTAAGC DsaI 498 CCATGG C^C[GA][CT]GG 651 CCGTGG FauI 808 CCCGC CCCGC(4/6) 2006 GCGGG MaeII 1754 ACGT A^CGT 1759 ACGT MflI 740 AGATCT [GA]^GATC[CT] 1007 AGATCT PstI 148 CTGCAG CTGCA^G 456 CTGCAG SmlI 980 CTCAAG C^T[CT][GA]AG 1933 CTCAAG StuI 1105 AGGCCT AGG^CCT 2012 AGGCCT TaqI 790 TCGA T^CGA 2724 TCGA TthHB8I 790 TCGA T^CGA 2724 TCGA XhoII 740 AGATCT [GA]^GATC[CT] 1007 AGATCT XmnI 2184 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2340 GAAGCTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI AspHI AvaII BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI Tru9I TseI Tsp45I Tsp509I TspRI XcmI