##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccII AccIII AflII AgeI
AlwI AscI AviII AvrII BamHI
BbeI BbrPI BclI BfrI BglI
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BsrBI BsrDI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I ClaI CpoI
DraIII EagI EclXI Eco52I EcoNI
EcoT22I FbaI FspI HpaI KasI
KspI MamI MluI MroI MvnI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SpeI
Sse8387I SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
Acc65I 262 GGTACC G^GTACC
AccI 985 GTCTAC GT^[AC][GT]AC
AclI 1753 AACGTT AA^CGTT
AcyI 1159 GACGCC G[GA]^CG[CT]C
AhdI 2397 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1924 GTGCAC G^TGCAC
AocI 181 CCTCAGG CC^T[ACGT]AGG
Aor51HI 953 AGCGCT AGC^GCT
ApaLI 1924 GTGCAC G^TGCAC
AseI 2254 ATTAAT AT^TAAT
AsnI 2254 ATTAAT AT^TAAT
Asp718I 262 GGTACC G^GTACC
AspEI 2397 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2925 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2907 TGGCCA TGG^CCA
BanI 262 GGTACC G^G[CT][GA]CC
BbvCI 1394 GCTGAGG CCTCAGC(-5/-2)
BcgI 1360 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2838 GTATCC GTATCC(6/5)
BsaAI 1758 TACGTA [CT]AC^GT[GA]
BsaHI 1159 GACGCC G[GA]^CG[CT]C
Bsp1407I 2776 TGTACA T^GTACA
BspHI 1464 TCATGA T^CATGA
BsrFI 1343 GCCGGC [GA]^CCGG[CT]
BsrGI 2776 TGTACA T^GTACA
BssSI 823 CACGAG CACGAG(-5/-1)
BstEII 107 GGTGACC G^GT[ACGT]ACC
BstPI 107 GGTGACC G^GT[ACGT]ACC
BstXI 1688 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 181 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1343 GCCGGC [GA]^CCGG[CT]
DrdI 1317 GACTGACGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2397 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 953 AGCGCT AGC^GCT
Eco81I 181 CCTCAGG CC^T[ACGT]AGG
EcoO65I 107 GGTGACC G^GT[ACGT]ACC
EcoRI 2184 GAATTC G^AATTC
EcoRV 3205 GATATC GAT^ATC
FseI 1342 GGCCGGCC GGCCGG^CC
HaeII 953 AGCGCT [GA]GCGC^[CT]
HgaI 1159 GACGC GACGC(5/10)
Hin1I 1159 GACGCC G[GA]^CG[CT]C
HincII 3165 GTTGAC GT[CT]^[GA]AC
HindII 3165 GTTGAC GT[CT]^[GA]AC
KpnI 262 GGTACC GGTAC^C
MfeI 3342 CAATTG C^AATTG
MluNI 2907 TGGCCA TGG^CCA
MscI 2907 TGGCCA TGG^CCA
MunI 3342 CAATTG C^AATTG
NaeI 1343 GCCGGC GCC^GGC
NcoI 498 CCATGG C^CATGG
NgoMIV 1343 GCCGGC G^CCGGC
PflFI 2925 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2565 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2254 ATTAAT AT^TAAT
Psp1406I 1753 AACGTT AA^CGTT
RcaI 1464 TCATGA T^CATGA
SapI 577 GAAGAGC GCTCTTC(1/4)
ScaI 1724 AGTACT AGT^ACT
SmaI 169 CCCGGG CCC^GGG
SnaBI 1758 TACGTA TAC^GTA
SphI 1191 GCATGC GCATG^C
SspBI 2776 TGTACA T^GTACA
TfiI 3019 GAATC G^A[AT]TC
Tth111I 2925 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 442 TCTAGA T^CTAGA
XmaCI 169 CCCGGG C^CCGGG
XmaI 169 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1716 GAATTT [GA]^AATT[CT]
2184 GAATTC
AflIII 1912 ACATGT A^C[GA][CT]GT
3065 ACATGT
ApoI 1716 GAATTT [GA]^AATT[CT]
2184 GAATTC
Asp700I 2184 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2340 GAAGCTTTTC
AvaI 169 CCCGGG C^[CT]CG[GA]G
396 CCCGAG
BbsI 716 GAAGAC GAAGAC(2/6)
776 GAAGAC
BglII 740 AGATCT A^GATCT
1007 AGATCT
BlpI 1253 GCTGAGC GC^T[ACGT]AGC
1972 GCTAAGC
BpmI 162 CTCCAG CTGGAG(16/14)
2194 CTGGAG
Bpu1102I 1253 GCTGAGC GC^T[ACGT]AGC
1972 GCTAAGC
BpuAI 716 GAAGAC GAAGAC(2/6)
776 GAAGAC
BsaI 1563 GGTCTC GGTCTC(1/5)
1578 GGTCTC
BsgI 120 GTGCAG GTGCAG(16/14)
2020 GTGCAG
BsoBI 169 CCCGGG C^[CT]CG[GA]G
396 CCCGAG
BspLU11I 1912 ACATGT A^CATGT
3065 ACATGT
BspMI 1249 ACCTGC ACCTGC(4/8)
1182 GCAGGT
BsrI 1876 ACTGG ACTGG(1/-1)
2871 CCAGT
BstYI 740 AGATCT [GA]^GATC[CT]
1007 AGATCT
CelII 1253 GCTGAGC GC^T[ACGT]AGC
1972 GCTAAGC
DsaI 498 CCATGG C^C[GA][CT]GG
651 CCGTGG
FauI 808 CCCGC CCCGC(4/6)
2006 GCGGG
MaeII 1754 ACGT A^CGT
1759 ACGT
MflI 740 AGATCT [GA]^GATC[CT]
1007 AGATCT
PstI 148 CTGCAG CTGCA^G
456 CTGCAG
SmlI 980 CTCAAG C^T[CT][GA]AG
1933 CTCAAG
StuI 1105 AGGCCT AGG^CCT
2012 AGGCCT
TaqI 790 TCGA T^CGA
2724 TCGA
TthHB8I 790 TCGA T^CGA
2724 TCGA
XhoII 740 AGATCT [GA]^GATC[CT]
1007 AGATCT
XmnI 2184 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2340 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaII BanII BbvI BcnI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI