##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AclI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AvrII BamHI BbeI BclI BglII
BlnI BsaI BsiEI Bsp1407I BspDI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FbaI FseI HaeII HgaI HpaI
KasI KpnI KspI MfeI MluI
MunI NarI NdeI NotI NruI
NsiI PacI PaeR7I PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SspBI SspI StuI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 1222 GACGTC GACGT^C
AccII 1838 CGCG CG^CG
AcyI 1222 GACGTC G[GA]^CG[CT]C
AflII 3260 CTTAAG C^TTAAG
AflIII 1556 ACGTGT A^C[GA][CT]GT
AgeI 712 ACCGGT A^CCGGT
AhdI 375 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 421 GGGCCC GGGCC^C
AspEI 375 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2567 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1825 GCTGAGG CCTCAGC(-5/-2)
BciVI 1481 GTATCC GTATCC(6/5)
BfrI 3260 CTTAAG C^TTAAG
BglI 3048 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2284 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2284 GCTAAGC GC^T[ACGT]AGC
BsaBI 1098 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1222 GACGTC G[GA]^CG[CT]C
BsgI 3280 CTGCAC GTGCAG(16/14)
BsiWI 1322 CGTACG C^GTACG
BsmBI 1932 CGTCTC CGTCTC(1/5)
BspHI 1430 TCATGA T^CATGA
BsrBI 539 CCGCTC CCGCTC(-3/-3)
BstBI 2730 TTCGAA TT^CGAA
BstEII 639 GGTGACC G^GT[ACGT]ACC
BstPI 639 GGTGACC G^GT[ACGT]ACC
BstUI 1838 CGCG CG^CG
BstYI 328 AGATCC [GA]^GATC[CT]
CelII 2284 GCTAAGC GC^T[ACGT]AGC
Eam1105I 375 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3229 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 639 GGTGACC G^GT[ACGT]ACC
Hin1I 1222 GACGTC G[GA]^CG[CT]C
HincII 3067 GTTGAC GT[CT]^[GA]AC
HindII 3067 GTTGAC GT[CT]^[GA]AC
MamI 1098 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 328 AGATCC [GA]^GATC[CT]
MvnI 1838 CGCG CG^CG
NaeI 1587 GCCGGC GCC^GGC
NgoMIV 1587 GCCGGC G^CCGGC
NheI 2984 GCTAGC G^CTAGC
NspV 2730 TTCGAA TT^CGAA
PflFI 2567 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1347 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 712 ACCGGT A^CCGGT
RcaI 1430 TCATGA T^CATGA
SacI 51 GAGCTC GAGCT^C
SbfI 2064 CCTGCAGG CCTGCA^GG
SfuI 2730 TTCGAA TT^CGAA
SpeI 2735 ACTAGT A^CTAGT
SphI 919 GCATGC GCATG^C
Sse8387I 2064 CCTGCAGG CCTGCA^GG
Tth111I 2567 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1347 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 328 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1131 GTCTAC GT^[AC][GT]AC
1327 GTCTAC
AccIII 187 TCCGGA T^CCGGA
3030 TCCGGA
Asp700I 1422 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2255 GAAACTCTTC
AvaI 135 CCCGAG C^[CT]CG[GA]G
1668 CCCGAG
AviII 1367 TGCGCA TGC^GCA
1550 TGCGCA
BalI 806 TGGCCA TGG^CCA
1181 TGGCCA
BanI 435 GGCACC G^G[CT][GA]CC
2141 GGTGCC
BbrPI 485 CACGTG CAC^GTG
1570 CACGTG
BcgI 1759 CGAGGAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2722 GCAGTCCCTTCG
BsaAI 485 CACGTG [CT]AC^GT[GA]
1570 CACGTG
BseAI 187 TCCGGA T^CCGGA
3030 TCCGGA
BsoBI 135 CCCGAG C^[CT]CG[GA]G
1668 CCCGAG
BspEI 187 TCCGGA T^CCGGA
3030 TCCGGA
BsrFI 712 ACCGGT [GA]^CCGG[CT]
1587 GCCGGC
BssSI 702 CACGAG CACGAG(-5/-1)
3248 CTCGTG
Cfr10I 712 ACCGGT [GA]^CCGG[CT]
1587 GCCGGC
EcoRI 756 GAATTC G^AATTC
2059 GAATTC
FspI 1367 TGCGCA TGC^GCA
1550 TGCGCA
HindIII 2762 AAGCTT A^AGCTT
3025 AAGCTT
MluNI 806 TGGCCA TGG^CCA
1181 TGGCCA
MroI 187 TCCGGA T^CCGGA
3030 TCCGGA
MscI 806 TGGCCA TGG^CCA
1181 TGGCCA
NspI 919 GCATGC [GA]CATG^[CT]
2932 GCATGT
PmaCI 485 CACGTG CAC^GTG
1570 CACGTG
PmlI 485 CACGTG CAC^GTG
1570 CACGTG
SapI 497 GCTCTTC GCTCTTC(1/4)
849 GCTCTTC
XcmI 1035 CCACCTCTATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1295 CCAGTGACATCATGG
XmnI 1422 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2255 GAAACTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I