################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## Acc65I AclI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AvrII BamHI BbeI BclI BglII BlnI BsaI BsiEI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI HaeII HgaI HpaI KasI KpnI KspI MfeI MluI MunI NarI NdeI NotI NruI NsiI PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SspBI SspI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 1222 GACGTC GACGT^C AccII 1838 CGCG CG^CG AcyI 1222 GACGTC G[GA]^CG[CT]C AflII 3260 CTTAAG C^TTAAG AflIII 1556 ACGTGT A^C[GA][CT]GT AgeI 712 ACCGGT A^CCGGT AhdI 375 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 421 GGGCCC GGGCC^C AspEI 375 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2567 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 1825 GCTGAGG CCTCAGC(-5/-2) BciVI 1481 GTATCC GTATCC(6/5) BfrI 3260 CTTAAG C^TTAAG BglI 3048 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2284 GCTAAGC GC^T[ACGT]AGC Bpu1102I 2284 GCTAAGC GC^T[ACGT]AGC BsaBI 1098 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1222 GACGTC G[GA]^CG[CT]C BsgI 3280 CTGCAC GTGCAG(16/14) BsiWI 1322 CGTACG C^GTACG BsmBI 1932 CGTCTC CGTCTC(1/5) BspHI 1430 TCATGA T^CATGA BsrBI 539 CCGCTC CCGCTC(-3/-3) BstBI 2730 TTCGAA TT^CGAA BstEII 639 GGTGACC G^GT[ACGT]ACC BstPI 639 GGTGACC G^GT[ACGT]ACC BstUI 1838 CGCG CG^CG BstYI 328 AGATCC [GA]^GATC[CT] CelII 2284 GCTAAGC GC^T[ACGT]AGC Eam1105I 375 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3229 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 639 GGTGACC G^GT[ACGT]ACC Hin1I 1222 GACGTC G[GA]^CG[CT]C HincII 3067 GTTGAC GT[CT]^[GA]AC HindII 3067 GTTGAC GT[CT]^[GA]AC MamI 1098 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 328 AGATCC [GA]^GATC[CT] MvnI 1838 CGCG CG^CG NaeI 1587 GCCGGC GCC^GGC NgoMIV 1587 GCCGGC G^CCGGC NheI 2984 GCTAGC G^CTAGC NspV 2730 TTCGAA TT^CGAA PflFI 2567 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1347 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 712 ACCGGT A^CCGGT RcaI 1430 TCATGA T^CATGA SacI 51 GAGCTC GAGCT^C SbfI 2064 CCTGCAGG CCTGCA^GG SfuI 2730 TTCGAA TT^CGAA SpeI 2735 ACTAGT A^CTAGT SphI 919 GCATGC GCATG^C Sse8387I 2064 CCTGCAGG CCTGCA^GG Tth111I 2567 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1347 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 328 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 1131 GTCTAC GT^[AC][GT]AC 1327 GTCTAC AccIII 187 TCCGGA T^CCGGA 3030 TCCGGA Asp700I 1422 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2255 GAAACTCTTC AvaI 135 CCCGAG C^[CT]CG[GA]G 1668 CCCGAG AviII 1367 TGCGCA TGC^GCA 1550 TGCGCA BalI 806 TGGCCA TGG^CCA 1181 TGGCCA BanI 435 GGCACC G^G[CT][GA]CC 2141 GGTGCC BbrPI 485 CACGTG CAC^GTG 1570 CACGTG BcgI 1759 CGAGGAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2722 GCAGTCCCTTCG BsaAI 485 CACGTG [CT]AC^GT[GA] 1570 CACGTG BseAI 187 TCCGGA T^CCGGA 3030 TCCGGA BsoBI 135 CCCGAG C^[CT]CG[GA]G 1668 CCCGAG BspEI 187 TCCGGA T^CCGGA 3030 TCCGGA BsrFI 712 ACCGGT [GA]^CCGG[CT] 1587 GCCGGC BssSI 702 CACGAG CACGAG(-5/-1) 3248 CTCGTG Cfr10I 712 ACCGGT [GA]^CCGG[CT] 1587 GCCGGC EcoRI 756 GAATTC G^AATTC 2059 GAATTC FspI 1367 TGCGCA TGC^GCA 1550 TGCGCA HindIII 2762 AAGCTT A^AGCTT 3025 AAGCTT MluNI 806 TGGCCA TGG^CCA 1181 TGGCCA MroI 187 TCCGGA T^CCGGA 3030 TCCGGA MscI 806 TGGCCA TGG^CCA 1181 TGGCCA NspI 919 GCATGC [GA]CATG^[CT] 2932 GCATGT PmaCI 485 CACGTG CAC^GTG 1570 CACGTG PmlI 485 CACGTG CAC^GTG 1570 CACGTG SapI 497 GCTCTTC GCTCTTC(1/4) 849 GCTCTTC XcmI 1035 CCACCTCTATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1295 CCAGTGACATCATGG XmnI 1422 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2255 GAAACTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I