##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AhdI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbrPI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsiWI BsmI BspDI
BspHI BspLU11I BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRI FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NotI
NruI NspV PacI PaeR7I PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI TfiI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 143 GTAGAC GT^[AC][GT]AC
AccIII 1611 TCCGGA T^CCGGA
AcyI 1295 GGCGTC G[GA]^CG[CT]C
AgeI 2791 ACCGGT A^CCGGT
Asp700I 2416 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1085 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 736 GGATCC G^GATCC
BcgI 1592 GCAAAGTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 1295 GGCGTC G[GA]^CG[CT]C
BseAI 1611 TCCGGA T^CCGGA
BsiEI 2408 CGGCCG CG[GA][CT]^CG
BsmBI 2846 GAGACG CGTCTC(1/5)
Bsp1407I 2646 TGTACA T^GTACA
BspEI 1611 TCCGGA T^CCGGA
BsrDI 1237 CATTGC GCAATG(2/0)
BsrGI 2646 TGTACA T^GTACA
EagI 2408 CGGCCG C^GGCCG
EclXI 2408 CGGCCG C^GGCCG
Eco52I 2408 CGGCCG C^GGCCG
EcoRV 886 GATATC GAT^ATC
EcoT22I 2033 ATGCAT ATGCA^T
Hin1I 1295 GGCGTC G[GA]^CG[CT]C
MroI 1611 TCCGGA T^CCGGA
NcoI 264 CCATGG C^CATGG
NdeI 899 CATATG CA^TATG
NheI 3445 GCTAGC G^CTAGC
NsiI 2033 ATGCAT ATGCA^T
PflFI 1085 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2658 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2791 ACCGGT A^CCGGT
PshAI 2912 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1072 GAGCTC GAGCT^C
SapI 1847 GAAGAGC GCTCTTC(1/4)
ScaI 2019 AGTACT AGT^ACT
SexAI 2733 ACCAGGT A^CC[AT]GGT
SspBI 2646 TGTACA T^GTACA
Tth111I 1085 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2658 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3348 CCAGCATGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2416 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 1652 CGCG CG^CG
2056 CGCG
BalI 203 TGGCCA TGG^CCA
2295 TGGCCA
BbsI 2051 GAAGAC GAAGAC(2/6)
2201 GTCTTC
BglI 2976 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3436 GCCTTGAGGGC
BpuAI 2051 GAAGAC GAAGAC(2/6)
2201 GTCTTC
BsaI 107 GAGACC GGTCTC(1/5)
1153 GGTCTC
BspMI 2687 ACCTGC ACCTGC(4/8)
3400 ACCTGC
BstUI 1652 CGCG CG^CG
2056 CGCG
BstYI 736 GGATCC [GA]^GATC[CT]
2145 GGATCT
EcoT14I 264 CCATGG C^C[AT][AT]GG
1989 CCAAGG
HaeII 486 AGCGCC [GA]GCGC^[CT]
2300 AGCGCC
MflI 736 GGATCC [GA]^GATC[CT]
2145 GGATCT
MluNI 203 TGGCCA TGG^CCA
2295 TGGCCA
MscI 203 TGGCCA TGG^CCA
2295 TGGCCA
MseI 3302 TTAA T^TAA
3581 TTAA
MslI 767 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2590 CATCCTCGTG
MvnI 1652 CGCG CG^CG
2056 CGCG
NaeI 969 GCCGGC GCC^GGC
2240 GCCGGC
NgoMIV 969 GCCGGC G^CCGGC
2240 GCCGGC
StyI 264 CCATGG C^C[AT][AT]GG
1989 CCAAGG
Tru9I 3302 TTAA T^TAA
3581 TTAA
Tsp45I 3052 GTGAC ^GT[GC]AC
3058 GTGAC
XhoII 736 GGATCC [GA]^GATC[CT]
2145 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstXI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
StuI TaqI TseI Tsp509I TspRI
TthHB8I XmaCI XmaI