################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AflIII AhdI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BbeI BbrPI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsiWI BsmI BspDI BspHI BspLU11I BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI TfiI XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 143 GTAGAC GT^[AC][GT]AC AccIII 1611 TCCGGA T^CCGGA AcyI 1295 GGCGTC G[GA]^CG[CT]C AgeI 2791 ACCGGT A^CCGGT Asp700I 2416 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1085 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 736 GGATCC G^GATCC BcgI 1592 GCAAAGTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 1295 GGCGTC G[GA]^CG[CT]C BseAI 1611 TCCGGA T^CCGGA BsiEI 2408 CGGCCG CG[GA][CT]^CG BsmBI 2846 GAGACG CGTCTC(1/5) Bsp1407I 2646 TGTACA T^GTACA BspEI 1611 TCCGGA T^CCGGA BsrDI 1237 CATTGC GCAATG(2/0) BsrGI 2646 TGTACA T^GTACA EagI 2408 CGGCCG C^GGCCG EclXI 2408 CGGCCG C^GGCCG Eco52I 2408 CGGCCG C^GGCCG EcoRV 886 GATATC GAT^ATC EcoT22I 2033 ATGCAT ATGCA^T Hin1I 1295 GGCGTC G[GA]^CG[CT]C MroI 1611 TCCGGA T^CCGGA NcoI 264 CCATGG C^CATGG NdeI 899 CATATG CA^TATG NheI 3445 GCTAGC G^CTAGC NsiI 2033 ATGCAT ATGCA^T PflFI 1085 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2658 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2791 ACCGGT A^CCGGT PshAI 2912 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1072 GAGCTC GAGCT^C SapI 1847 GAAGAGC GCTCTTC(1/4) ScaI 2019 AGTACT AGT^ACT SexAI 2733 ACCAGGT A^CC[AT]GGT SspBI 2646 TGTACA T^GTACA Tth111I 1085 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2658 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 3348 CCAGCATGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2416 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 1652 CGCG CG^CG 2056 CGCG BalI 203 TGGCCA TGG^CCA 2295 TGGCCA BbsI 2051 GAAGAC GAAGAC(2/6) 2201 GTCTTC BglI 2976 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3436 GCCTTGAGGGC BpuAI 2051 GAAGAC GAAGAC(2/6) 2201 GTCTTC BsaI 107 GAGACC GGTCTC(1/5) 1153 GGTCTC BspMI 2687 ACCTGC ACCTGC(4/8) 3400 ACCTGC BstUI 1652 CGCG CG^CG 2056 CGCG BstYI 736 GGATCC [GA]^GATC[CT] 2145 GGATCT EcoT14I 264 CCATGG C^C[AT][AT]GG 1989 CCAAGG HaeII 486 AGCGCC [GA]GCGC^[CT] 2300 AGCGCC MflI 736 GGATCC [GA]^GATC[CT] 2145 GGATCT MluNI 203 TGGCCA TGG^CCA 2295 TGGCCA MscI 203 TGGCCA TGG^CCA 2295 TGGCCA MseI 3302 TTAA T^TAA 3581 TTAA MslI 767 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2590 CATCCTCGTG MvnI 1652 CGCG CG^CG 2056 CGCG NaeI 969 GCCGGC GCC^GGC 2240 GCCGGC NgoMIV 969 GCCGGC G^CCGGC 2240 GCCGGC StyI 264 CCATGG C^C[AT][AT]GG 1989 CCAAGG Tru9I 3302 TTAA T^TAA 3581 TTAA Tsp45I 3052 GTGAC ^GT[GC]AC 3058 GTGAC XhoII 736 GGATCC [GA]^GATC[CT] 2145 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AciI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstXI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StuI TaqI TseI Tsp509I TspRI TthHB8I XmaCI XmaI