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# NO CUT ENZYMES ( 214 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoRV EcoT14I FauI
FbaI Fnu4HI FseI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
ItaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI ScrFI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 338 GTAC GT^AC
AlwI 3 GGATC GGATC(4/5)
AlwNI 66 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 562 ATTAAT AT^TAAT
AsnI 562 ATTAAT AT^TAAT
AspHI 50 GTGCTC G[AT]GC[AT]^C
BmyI 50 GTGCTC G[AGT]GC[ACT]^C
BsaJI 456 CCACGG C^C[ACGT][ACGT]GG
BsiHKAI 50 GTGCTC G[AT]GC[AT]^C
BsmI 274 GAATGC GAATGC(1/-1)
Bsp1286I 50 GTGCTC G[AGT]GC[ACT]^C
BspHI 303 TCATGA T^CATGA
BsrDI 406 GCAATG GCAATG(2/0)
DpnII 4 GATC ^GATC
DraI 542 TTTAAA TTT^AAA
DsaI 456 CCACGG C^C[GA][CT]GG
EcoRI 166 GAATTC G^AATTC
EcoT22I 276 ATGCAT ATGCA^T
HindIII 178 AAGCTT A^AGCTT
HphI 395 GGTGA GGTGA(8/7)
MboI 4 GATC ^GATC
NdeII 4 GATC ^GATC
NsiI 276 ATGCAT ATGCA^T
PshBI 562 ATTAAT AT^TAAT
RcaI 303 TCATGA T^CATGA
RsaI 338 GTAC GT^AC
Sau3AI 4 GATC ^GATC
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
AluI 105 AGCT AG^CT
179 AGCT
BsiYI 17 CCACTTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
386 CCTTTCTGTGG
BslI 17 CCACTTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
386 CCTTTCTGTGG
BstF5I 453 CATCC GGATG(2/0)
190 GGATG
FokI 453 CATCC GGATG(9/13)
190 GGATG
HinfI 27 GAGTC G^A[ACGT]TC
126 GAGTC
PleI 27 GAGTC GAGTC(4/5)
126 GAGTC
SfaNI 68 GATGC GCATC(5/9)
452 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AcsI ApoI DdeI MboII MnlI
MseI NlaIII Tru9I Tsp509I