################################## # NO CUT ENZYMES ( 214 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AfaI 338 GTAC GT^AC AlwI 3 GGATC GGATC(4/5) AlwNI 66 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 562 ATTAAT AT^TAAT AsnI 562 ATTAAT AT^TAAT AspHI 50 GTGCTC G[AT]GC[AT]^C BmyI 50 GTGCTC G[AGT]GC[ACT]^C BsaJI 456 CCACGG C^C[ACGT][ACGT]GG BsiHKAI 50 GTGCTC G[AT]GC[AT]^C BsmI 274 GAATGC GAATGC(1/-1) Bsp1286I 50 GTGCTC G[AGT]GC[ACT]^C BspHI 303 TCATGA T^CATGA BsrDI 406 GCAATG GCAATG(2/0) DpnII 4 GATC ^GATC DraI 542 TTTAAA TTT^AAA DsaI 456 CCACGG C^C[GA][CT]GG EcoRI 166 GAATTC G^AATTC EcoT22I 276 ATGCAT ATGCA^T HindIII 178 AAGCTT A^AGCTT HphI 395 GGTGA GGTGA(8/7) MboI 4 GATC ^GATC NdeII 4 GATC ^GATC NsiI 276 ATGCAT ATGCA^T PshBI 562 ATTAAT AT^TAAT RcaI 303 TCATGA T^CATGA RsaI 338 GTAC GT^AC Sau3AI 4 GATC ^GATC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AluI 105 AGCT AG^CT 179 AGCT BsiYI 17 CCACTTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 386 CCTTTCTGTGG BslI 17 CCACTTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 386 CCTTTCTGTGG BstF5I 453 CATCC GGATG(2/0) 190 GGATG FokI 453 CATCC GGATG(9/13) 190 GGATG HinfI 27 GAGTC G^A[ACGT]TC 126 GAGTC PleI 27 GAGTC GAGTC(4/5) 126 GAGTC SfaNI 68 GATGC GCATC(5/9) 452 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AcsI ApoI DdeI MboII MnlI MseI NlaIII Tru9I Tsp509I