##################################
# NO CUT ENZYMES ( 176 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmFI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
HaeII HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MamI MboI MfeI MflI
MluI MroI MseI MspA1I MunI
MvnI NarI NciI NcoI NdeII
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TfiI
Tru9I Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 295 GACGTC GACGT^C
Asp700I 25 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 389 GGACC G^G[AT]CC
AviII 204 TGCGCA TGC^GCA
BalI 282 TGGCCA TGG^CCA
BbsI 377 GTCTTC GAAGAC(2/6)
BglI 343 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 377 GTCTTC GAAGAC(2/6)
BsaAI 394 TACGTG [CT]AC^GT[GA]
BseRI 253 CTCCTC GAGGAG(10/8)
BsgI 68 CTGCAC GTGCAG(16/14)
BsiYI 260 CCAATTAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 260 CCAATTAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 293 GAGACG CGTCTC(1/5)
Bsp1407I 42 TGTACA T^GTACA
BsrFI 138 GCCGGC [GA]^CCGG[CT]
BsrGI 42 TGTACA T^GTACA
BstAPI 12 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 138 GCCGGC [GA]^CCGG[CT]
Cfr13I 389 GGACC G^G[ACGT]CC
DraII 388 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 282 TGGCCA [CT]^GGCC[GA]
EcoO109I 388 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 21 GCGGG CCCGC(4/6)
FspI 204 TGCGCA TGC^GCA
HaeIII 283 GGCC GG^CC
HapII 139 CCGG C^CGG
HgaI 162 GCGTC GACGC(5/10)
HinfI 249 GAGTC G^A[ACGT]TC
HpaII 139 CCGG C^CGG
MaeIII 355 GTAAC ^GT[ACGT]AC
MluNI 282 TGGCCA TGG^CCA
MscI 282 TGGCCA TGG^CCA
MslI 87 CATTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 139 CCGG C^CGG
NaeI 138 GCCGGC GCC^GGC
NdeI 59 CATATG CA^TATG
NgoMIV 138 GCCGGC G^CCGGC
NlaIV 331 GGAACC GG[ACGT]^[ACGT]CC
NspI 39 GCATGT [GA]CATG^[CT]
PleI 249 GAGTC GAGTC(4/5)
PpuMI 388 AGGACCT [GA]G^G[AT]CC[CT]
Sau96I 389 GGACC G^G[ACGT]CC
SmlI 122 CTCAAG C^T[CT][GA]AG
SspBI 42 TGTACA T^GTACA
Tsp509I 262 AATT ^AATT
TspRI 208 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 25 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 37 CCGC CCGC(-3/-1)
21 GCGG
AcyI 161 GGCGTC G[GA]^CG[CT]C
295 GACGTC
AfaI 43 GTAC GT^AC
100 GTAC
BanII 309 GGGCTC G[GA]GC[CT]^C
399 GGGCTC
BbvI 78 GCAGC GCAGC(8/12)
231 GCAGC
BsaHI 161 GGCGTC G[GA]^CG[CT]C
295 GACGTC
BsmAI 293 GAGAC GTCTC(1/5)
251 GTCTC
BspMI 48 ACCTGC ACCTGC(4/8)
66 ACCTGC
BssKI 178 CCTGG ^CC[ACGT]GG
335 CCTGG
BstNI 178 CCTGG CC^[AT]GG
335 CCTGG
CfoI 205 GCGC GCG^C
239 GCGC
EcoRII 178 CCTGG ^CC[AT]GG
335 CCTGG
Fnu4HI 78 GCAGC GC^[ACGT]GC
231 GCAGC
HhaI 205 GCGC GCG^C
239 GCGC
Hin1I 161 GGCGTC G[GA]^CG[CT]C
295 GACGTC
HinP1I 205 GCGC G^CGC
239 GCGC
ItaI 78 GCAGC GC^[ACGT]GC
231 GCAGC
MaeII 296 ACGT A^CGT
395 ACGT
MboII 378 TCTTC GAAGA(8/7)
381 TCTTC
MvaI 178 CCTGG CC^[AT]GG
335 CCTGG
RsaI 43 GTAC GT^AC
100 GTAC
ScrFI 178 CCTGG CC^[ACGT]GG
335 CCTGG
TseI 78 GCAGC G^C[AT]GC
231 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BmyI BpmI BsaJI Bsp1286I Cac8I
DdeI EcoT14I MnlI MwoI NlaIII
StyI