##################################
# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BanI BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII Hin1I HincII
HindII HpaI HpaII HphI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI TaqI TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 362 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 382 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 536 GGATCC G^GATCC
BanII 355 GAGCTC G[GA]GC[CT]^C
BbsI 379 GAAGAC GAAGAC(2/6)
BbvCI 748 GCTGAGG CCTCAGC(-5/-2)
BpuAI 379 GAAGAC GAAGAC(2/6)
BsgI 614 GTGCAG GTGCAG(16/14)
BsmI 681 GCATTC GAATGC(1/-1)
Bsp1407I 153 TGTACA T^GTACA
BsrGI 153 TGTACA T^GTACA
BssSI 663 CTCGTG CACGAG(-5/-1)
CfoI 143 GCGC GCG^C
EarI 247 CTCTTC CTCTTC(1/4)
HgaI 841 GACGC GACGC(5/10)
HhaI 143 GCGC GCG^C
HinP1I 143 GCGC G^CGC
HindIII 435 AAGCTT A^AGCTT
Ksp632I 247 CTCTTC CTCTTC(1/4)
MaeII 27 ACGT A^CGT
MseI 855 TTAA T^TAA
MspA1I 597 CAGCTG C[AC]G^C[GT]G
MwoI 236 GCCGAGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI 382 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 597 CAGCTG CAG^CTG
SacI 355 GAGCTC GAGCT^C
SmlI 626 CTTGAG C^T[CT][GA]AG
SspBI 153 TGTACA T^GTACA
StuI 319 AGGCCT AGG^CCT
Tru9I 855 TTAA T^TAA
Tsp509I 736 AATT ^AATT
Tth111I 382 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
Alw44I 23 GTGCAC G^TGCAC
666 GTGCAC
ApaLI 23 GTGCAC G^TGCAC
666 GTGCAC
BseRI 170 GAGGAG GAGGAG(10/8)
257 GAGGAG
BsmFI 444 GGGAC GGGAC(10/14)
654 GTCCC
Cac8I 82 GCCTGC GC[ACGT]^[ACGT]GC
544 GCTGGC
PleI 661 GACTC GAGTC(4/5)
68 GAGTC
PpuMI 223 AGGACCC [GA]G^G[AT]CC[CT]
652 GGGTCCC
PstI 7 CTGCAG CTGCA^G
74 CTGCAG
SfcI 7 CTGCAG C^T[GA][CT]AG
74 CTGCAG
TfiI 515 GAATC G^A[AT]TC
604 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeIII
MboI MboII MflI MnlI MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TseI Tsp45I TspRI
XhoII