##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmFI BspDI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HpaI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AciI 181 GCGG CCGC(-3/-1)
Alw44I 577 GTGCAC G^TGCAC
AlwNI 761 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 577 GTGCAC G^TGCAC
AspHI 577 GTGCAC G[AT]GC[AT]^C
AvaI 745 CTCGGG C^[CT]CG[GA]G
BanI 618 GGCGCC G^G[CT][GA]CC
BanII 456 GGGCTC G[GA]GC[CT]^C
BbeI 618 GGCGCC GGCGC^C
BcgI 444 GCACTGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 394 CCCGG CC^[GC]GG
BglI 449 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 11 GCTCAGC GC^T[ACGT]AGC
BpmI 153 CTCCAG CTGGAG(16/14)
Bpu1102I 11 GCTCAGC GC^T[ACGT]AGC
BsiEI 310 CGATCG CG[GA][CT]^CG
BsiHKAI 577 GTGCAC G[AT]GC[AT]^C
BsmBI 842 CGTCTC CGTCTC(1/5)
BsmI 116 GAATGC GAATGC(1/-1)
BsoBI 745 CTCGGG C^[CT]CG[GA]G
Bsp1407I 739 TGTACA T^GTACA
BsrDI 502 GCAATG GCAATG(2/0)
BsrGI 739 TGTACA T^GTACA
BstXI 126 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 807 AGATCC [GA]^GATC[CT]
CelII 11 GCTCAGC GC^T[ACGT]AGC
CfoI 619 GCGC GCG^C
EaeI 733 CGGCCA [CT]^GGCC[GA]
EcoT14I 38 CCTTGG C^C[AT][AT]GG
HaeII 618 GGCGCC [GA]GCGC^[CT]
HgaI 244 GACGC GACGC(5/10)
HhaI 619 GCGC GCG^C
HinP1I 619 GCGC G^CGC
HincII 105 GTCAAC GT[CT]^[GA]AC
HindII 105 GTCAAC GT[CT]^[GA]AC
HindIII 378 AAGCTT A^AGCTT
KasI 618 GGCGCC G^GCGCC
MboII 279 TCTTC GAAGA(8/7)
MflI 807 AGATCC [GA]^GATC[CT]
MslI 674 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 618 GGCGCC GG^CGCC
NciI 394 CCCGG CC^[GC]GG
PpuMI 301 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 522 CTGCAG CTGCA^G
PvuI 310 CGATCG CGAT^CG
ScaI 568 AGTACT AGT^ACT
SfaNI 591 GATGC GCATC(5/9)
SfcI 522 CTGCAG C^T[GA][CT]AG
SfoI 618 GGCGCC GGC^GCC
SspBI 739 TGTACA T^GTACA
StyI 38 CCTTGG C^C[AT][AT]GG
TaqI 309 TCGA T^CGA
Tsp509I 101 AATT ^AATT
TthHB8I 309 TCGA T^CGA
XhoII 807 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 244 GACGCC G[GA]^CG[CT]C
618 GGCGCC
AlwI 808 GATCC GGATC(4/5)
769 GGATC
AvaII 32 GGTCC G^G[AT]CC
302 GGTCC
BbvI 524 GCAGC GCAGC(8/12)
219 GCTGC
BsaHI 244 GACGCC G[GA]^CG[CT]C
618 GGCGCC
BseRI 44 CTCCTC GAGGAG(10/8)
623 CTCCTC
BsrFI 165 ACCGGC [GA]^CCGG[CT]
731 ACCGGC
Cfr10I 165 ACCGGC [GA]^CCGG[CT]
731 ACCGGC
DraII 301 AGGTCCT [GA]G^G[ACGT]CC[CT]
647 AGGCCCT
EcoO109I 301 AGGTCCT [GA]G^G[ACGT]CC[CT]
647 AGGCCCT
Fnu4HI 219 GCTGC GC^[ACGT]GC
524 GCAGC
Hin1I 244 GACGCC G[GA]^CG[CT]C
618 GGCGCC
ItaI 219 GCTGC GC^[ACGT]GC
524 GCAGC
PflMI 472 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
776 CCAAGTCATGG
PleI 368 GACTC GAGTC(4/5)
639 GACTC
TfiI 487 GAATC G^A[AT]TC
554 GAATC
TseI 219 GCTGC G^C[AT]GC
524 GCAGC
Van91I 472 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
776 CCAAGTCATGG
XcmI 173 CCACTTCTGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
230 CCAAGTCACGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AfaI AluI BmyI BsaJI BsgI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII HaeIII HapII
HinfI HpaII HphI MaeIII MboI
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI Tsp45I TspRI