################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmFI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HpaI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AciI 181 GCGG CCGC(-3/-1) Alw44I 577 GTGCAC G^TGCAC AlwNI 761 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 577 GTGCAC G^TGCAC AspHI 577 GTGCAC G[AT]GC[AT]^C AvaI 745 CTCGGG C^[CT]CG[GA]G BanI 618 GGCGCC G^G[CT][GA]CC BanII 456 GGGCTC G[GA]GC[CT]^C BbeI 618 GGCGCC GGCGC^C BcgI 444 GCACTGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 394 CCCGG CC^[GC]GG BglI 449 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 11 GCTCAGC GC^T[ACGT]AGC BpmI 153 CTCCAG CTGGAG(16/14) Bpu1102I 11 GCTCAGC GC^T[ACGT]AGC BsiEI 310 CGATCG CG[GA][CT]^CG BsiHKAI 577 GTGCAC G[AT]GC[AT]^C BsmBI 842 CGTCTC CGTCTC(1/5) BsmI 116 GAATGC GAATGC(1/-1) BsoBI 745 CTCGGG C^[CT]CG[GA]G Bsp1407I 739 TGTACA T^GTACA BsrDI 502 GCAATG GCAATG(2/0) BsrGI 739 TGTACA T^GTACA BstXI 126 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 807 AGATCC [GA]^GATC[CT] CelII 11 GCTCAGC GC^T[ACGT]AGC CfoI 619 GCGC GCG^C EaeI 733 CGGCCA [CT]^GGCC[GA] EcoT14I 38 CCTTGG C^C[AT][AT]GG HaeII 618 GGCGCC [GA]GCGC^[CT] HgaI 244 GACGC GACGC(5/10) HhaI 619 GCGC GCG^C HinP1I 619 GCGC G^CGC HincII 105 GTCAAC GT[CT]^[GA]AC HindII 105 GTCAAC GT[CT]^[GA]AC HindIII 378 AAGCTT A^AGCTT KasI 618 GGCGCC G^GCGCC MboII 279 TCTTC GAAGA(8/7) MflI 807 AGATCC [GA]^GATC[CT] MslI 674 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 618 GGCGCC GG^CGCC NciI 394 CCCGG CC^[GC]GG PpuMI 301 AGGTCCT [GA]G^G[AT]CC[CT] PstI 522 CTGCAG CTGCA^G PvuI 310 CGATCG CGAT^CG ScaI 568 AGTACT AGT^ACT SfaNI 591 GATGC GCATC(5/9) SfcI 522 CTGCAG C^T[GA][CT]AG SfoI 618 GGCGCC GGC^GCC SspBI 739 TGTACA T^GTACA StyI 38 CCTTGG C^C[AT][AT]GG TaqI 309 TCGA T^CGA Tsp509I 101 AATT ^AATT TthHB8I 309 TCGA T^CGA XhoII 807 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 244 GACGCC G[GA]^CG[CT]C 618 GGCGCC AlwI 808 GATCC GGATC(4/5) 769 GGATC AvaII 32 GGTCC G^G[AT]CC 302 GGTCC BbvI 524 GCAGC GCAGC(8/12) 219 GCTGC BsaHI 244 GACGCC G[GA]^CG[CT]C 618 GGCGCC BseRI 44 CTCCTC GAGGAG(10/8) 623 CTCCTC BsrFI 165 ACCGGC [GA]^CCGG[CT] 731 ACCGGC Cfr10I 165 ACCGGC [GA]^CCGG[CT] 731 ACCGGC DraII 301 AGGTCCT [GA]G^G[ACGT]CC[CT] 647 AGGCCCT EcoO109I 301 AGGTCCT [GA]G^G[ACGT]CC[CT] 647 AGGCCCT Fnu4HI 219 GCTGC GC^[ACGT]GC 524 GCAGC Hin1I 244 GACGCC G[GA]^CG[CT]C 618 GGCGCC ItaI 219 GCTGC GC^[ACGT]GC 524 GCAGC PflMI 472 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 776 CCAAGTCATGG PleI 368 GACTC GAGTC(4/5) 639 GACTC TfiI 487 GAATC G^A[AT]TC 554 GAATC TseI 219 GCTGC G^C[AT]GC 524 GCAGC Van91I 472 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 776 CCAAGTCATGG XcmI 173 CCACTTCTGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 230 CCAAGTCACGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AfaI AluI BmyI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII HaeIII HapII HinfI HpaII HphI MaeIII MboI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI Tsp45I TspRI