################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbrPI BciVI BclI BfrI BglI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraIII EagI EarI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 1590 GTCTAC GT^[AC][GT]AC AhdI 715 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 643 GTGCAC G^TGCAC Aor51HI 932 AGCGCT AGC^GCT ApaLI 643 GTGCAC G^TGCAC AspEI 715 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 256 TGGCCA TGG^CCA BbsI 70 GTCTTC GAAGAC(2/6) BcgI 1399 GCACCACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 392 AGATCT A^GATCT BpuAI 70 GTCTTC GAAGAC(2/6) BsaI 26 GAGACC GGTCTC(1/5) BsmBI 549 CGTCTC CGTCTC(1/5) BssSI 828 CACGAG CACGAG(-5/-1) BstYI 392 AGATCT [GA]^GATC[CT] DraI 2002 TTTAAA TTT^AAA EaeI 256 TGGCCA [CT]^GGCC[GA] Eam1105I 715 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 932 AGCGCT AGC^GCT HgaI 1695 GACGC GACGC(5/10) MaeII 1721 ACGT A^CGT MflI 392 AGATCT [GA]^GATC[CT] MluNI 256 TGGCCA TGG^CCA MscI 256 TGGCCA TGG^CCA NcoI 1848 CCATGG C^CATGG PaeR7I 1646 CTCGAG C^TCGAG PleI 1676 GACTC GAGTC(4/5) PshAI 1506 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 143 ACCTGGT A^CC[AT]GGT SmaI 320 CCCGGG CCC^GGG StuI 364 AGGCCT AGG^CCT TaqI 1647 TCGA T^CGA TthHB8I 1647 TCGA T^CGA XbaI 1577 TCTAGA T^CTAGA XhoI 1646 CTCGAG C^TCGAG XhoII 392 AGATCT [GA]^GATC[CT] XmaCI 320 CCCGGG C^CCGGG XmaI 320 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AlwI 387 GGATC GGATC(4/5) 1567 GGATC ApaI 663 GGGCCC GGGCC^C 1193 GGGCCC AvaI 320 CCCGGG C^[CT]CG[GA]G 1646 CTCGAG BanI 1045 GGCGCC G^G[CT][GA]CC 1445 GGCGCC BbeI 1045 GGCGCC GGCGC^C 1445 GGCGCC BbvCI 327 GCTGAGG CCTCAGC(-5/-2) 1599 GCTGAGG BlpI 81 GCTCAGC GC^T[ACGT]AGC 1176 GCTCAGC Bpu1102I 81 GCTCAGC GC^T[ACGT]AGC 1176 GCTCAGC BsmFI 499 GGGAC GGGAC(10/14) 686 GGGAC BsoBI 320 CCCGGG C^[CT]CG[GA]G 1646 CTCGAG Bsp1407I 185 TGTACA T^GTACA 1454 TGTACA BsrGI 185 TGTACA T^GTACA 1454 TGTACA BstXI 1089 CCAGAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1402 CCACTGTCGTGG CelII 81 GCTCAGC GC^T[ACGT]AGC 1176 GCTCAGC DrdI 216 GACACTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1581 GACTGGCAGGTC EcoNI 1779 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2068 CCTGTCACAGG FauI 1855 CCCGC CCCGC(4/6) 94 GCGGG HapII 321 CCGG C^CGG 1759 CCGG HpaII 321 CCGG C^CGG 1759 CCGG KasI 1045 GGCGCC G^GCGCC 1445 GGCGCC MboII 866 GAAGA GAAGA(8/7) 71 TCTTC MseI 2003 TTAA T^TAA 2010 TTAA MspI 321 CCGG C^CGG 1759 CCGG NarI 1045 GGCGCC GG^CGCC 1445 GGCGCC PflMI 490 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1791 CCAACAGCTGG SacI 272 GAGCTC GAGCT^C 994 GAGCTC SfoI 1045 GGCGCC GGC^GCC 1445 GGCGCC SphI 90 GCATGC GCATG^C 1349 GCATGC SspBI 185 TGTACA T^GTACA 1454 TGTACA Tru9I 2003 TTAA T^TAA 2010 TTAA Tsp509I 1992 AATT ^AATT 2013 AATT Van91I 490 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1791 CCAACAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AcyI AfaI AluI AlwNI AocI AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HhaI Hin1I HinP1I HincII HindII HinfI HphI ItaI MaeI MaeIII MboI MnlI MslI MspA1I MvaI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI TseI Tsp45I TspRI XcmI