##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BbrPI
BciVI BclI BfrI BglI BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Cfr10I ClaI CpoI DraIII
EagI EarI EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1590 GTCTAC GT^[AC][GT]AC
AhdI 715 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 643 GTGCAC G^TGCAC
Aor51HI 932 AGCGCT AGC^GCT
ApaLI 643 GTGCAC G^TGCAC
AspEI 715 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 256 TGGCCA TGG^CCA
BbsI 70 GTCTTC GAAGAC(2/6)
BcgI 1399 GCACCACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 392 AGATCT A^GATCT
BpuAI 70 GTCTTC GAAGAC(2/6)
BsaI 26 GAGACC GGTCTC(1/5)
BsmBI 549 CGTCTC CGTCTC(1/5)
BssSI 828 CACGAG CACGAG(-5/-1)
BstYI 392 AGATCT [GA]^GATC[CT]
DraI 2002 TTTAAA TTT^AAA
EaeI 256 TGGCCA [CT]^GGCC[GA]
Eam1105I 715 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 932 AGCGCT AGC^GCT
HgaI 1695 GACGC GACGC(5/10)
MaeII 1721 ACGT A^CGT
MflI 392 AGATCT [GA]^GATC[CT]
MluNI 256 TGGCCA TGG^CCA
MscI 256 TGGCCA TGG^CCA
NcoI 1848 CCATGG C^CATGG
PaeR7I 1646 CTCGAG C^TCGAG
PleI 1676 GACTC GAGTC(4/5)
PshAI 1506 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 143 ACCTGGT A^CC[AT]GGT
SmaI 320 CCCGGG CCC^GGG
StuI 364 AGGCCT AGG^CCT
TaqI 1647 TCGA T^CGA
TthHB8I 1647 TCGA T^CGA
XbaI 1577 TCTAGA T^CTAGA
XhoI 1646 CTCGAG C^TCGAG
XhoII 392 AGATCT [GA]^GATC[CT]
XmaCI 320 CCCGGG C^CCGGG
XmaI 320 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 387 GGATC GGATC(4/5)
1567 GGATC
ApaI 663 GGGCCC GGGCC^C
1193 GGGCCC
AvaI 320 CCCGGG C^[CT]CG[GA]G
1646 CTCGAG
BanI 1045 GGCGCC G^G[CT][GA]CC
1445 GGCGCC
BbeI 1045 GGCGCC GGCGC^C
1445 GGCGCC
BbvCI 327 GCTGAGG CCTCAGC(-5/-2)
1599 GCTGAGG
BlpI 81 GCTCAGC GC^T[ACGT]AGC
1176 GCTCAGC
Bpu1102I 81 GCTCAGC GC^T[ACGT]AGC
1176 GCTCAGC
BsmFI 499 GGGAC GGGAC(10/14)
686 GGGAC
BsoBI 320 CCCGGG C^[CT]CG[GA]G
1646 CTCGAG
Bsp1407I 185 TGTACA T^GTACA
1454 TGTACA
BsrGI 185 TGTACA T^GTACA
1454 TGTACA
BstXI 1089 CCAGAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1402 CCACTGTCGTGG
CelII 81 GCTCAGC GC^T[ACGT]AGC
1176 GCTCAGC
DrdI 216 GACACTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1581 GACTGGCAGGTC
EcoNI 1779 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2068 CCTGTCACAGG
FauI 1855 CCCGC CCCGC(4/6)
94 GCGGG
HapII 321 CCGG C^CGG
1759 CCGG
HpaII 321 CCGG C^CGG
1759 CCGG
KasI 1045 GGCGCC G^GCGCC
1445 GGCGCC
MboII 866 GAAGA GAAGA(8/7)
71 TCTTC
MseI 2003 TTAA T^TAA
2010 TTAA
MspI 321 CCGG C^CGG
1759 CCGG
NarI 1045 GGCGCC GG^CGCC
1445 GGCGCC
PflMI 490 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1791 CCAACAGCTGG
SacI 272 GAGCTC GAGCT^C
994 GAGCTC
SfoI 1045 GGCGCC GGC^GCC
1445 GGCGCC
SphI 90 GCATGC GCATG^C
1349 GCATGC
SspBI 185 TGTACA T^GTACA
1454 TGTACA
Tru9I 2003 TTAA T^TAA
2010 TTAA
Tsp509I 1992 AATT ^AATT
2013 AATT
Van91I 490 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1791 CCAACAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AocI AspHI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HhaI Hin1I HinP1I HincII HindII
HinfI HphI ItaI MaeI MaeIII
MboI MnlI MslI MspA1I MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI TseI Tsp45I
TspRI XcmI