##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII BamHI BbrPI
BbsI BcgI BciVI BclI BcnI
BglI BglII BpuAI BsaAI BsaBI
BsaI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspHI BspMI BsrDI
BssHII BssSI Bst1107I BstUI BstXI
BstYI BstZ17I ClaI CpoI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoT22I FbaI FseI
FspI HincII HindII HpaI KpnI
KspI MamI MfeI MflI MluI
MunI MvnI NciI NcoI NdeI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AcsI 1797 AAATTC [GA]^AATT[CT]
AcyI 15 GGCGCC G[GA]^CG[CT]C
AflIII 230 ACATGT A^C[GA][CT]GT
AlwNI 1568 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 722 GGGCCC GGGCC^C
ApoI 1797 AAATTC [GA]^AATT[CT]
AspHI 551 GAGCTC G[AT]GC[AT]^C
AspI 1524 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 173 TGGCCA TGG^CCA
BbeI 15 GGCGCC GGCGC^C
BpmI 615 CTGGAG CTGGAG(16/14)
BsaHI 15 GGCGCC G[GA]^CG[CT]C
BsgI 534 CTGCAC GTGCAG(16/14)
BsiHKAI 551 GAGCTC G[AT]GC[AT]^C
Bsp1407I 1336 TGTACA T^GTACA
BspLU11I 230 ACATGT A^CATGT
BsrBI 814 CCGCTC CCGCTC(-3/-3)
BsrFI 284 GCCGGC [GA]^CCGG[CT]
BsrGI 1336 TGTACA T^GTACA
BstAPI 979 GCAAGTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1074 GGTGACC G^GT[ACGT]ACC
BstPI 1074 GGTGACC G^GT[ACGT]ACC
Cfr10I 284 GCCGGC [GA]^CCGG[CT]
DpnII 697 GATC ^GATC
DraI 844 TTTAAA TTT^AAA
DraII 661 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 1454 GACTCACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 173 TGGCCA [CT]^GGCC[GA]
EcoNI 1501 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 661 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1074 GGTGACC G^GT[ACGT]ACC
EcoRV 585 GATATC GAT^ATC
HaeII 15 GGCGCC [GA]GCGC^[CT]
HgaI 157 GACGC GACGC(5/10)
Hin1I 15 GGCGCC G[GA]^CG[CT]C
HindIII 848 AAGCTT A^AGCTT
KasI 15 GGCGCC G^GCGCC
MaeII 677 ACGT A^CGT
MboI 697 GATC ^GATC
MluNI 173 TGGCCA TGG^CCA
MscI 173 TGGCCA TGG^CCA
NaeI 284 GCCGGC GCC^GGC
NarI 15 GGCGCC GG^CGCC
NdeII 697 GATC ^GATC
NgoMIV 284 GCCGGC G^CCGGC
NheI 501 GCTAGC G^CTAGC
PflFI 1524 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 386 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 193 CTGCAG CTGCA^G
SacI 551 GAGCTC GAGCT^C
Sau3AI 697 GATC ^GATC
SexAI 1763 ACCTGGT A^CC[AT]GGT
SfcI 193 CTGCAG C^T[GA][CT]AG
SfoI 15 GGCGCC GGC^GCC
SspBI 1336 TGTACA T^GTACA
TfiI 141 GAATC G^A[AT]TC
Tth111I 1524 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 386 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 382 CCAACCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 52 TCCGGA T^CCGGA
547 TCCGGA
AflII 569 CTTAAG C^TTAAG
1649 CTTAAG
Asp700I 398 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1793 GAATAAATTC
AvrII 715 CCTAGG C^CTAGG
1443 CCTAGG
BbvCI 1586 CCTCAGC CCTCAGC(-5/-2)
1693 GCTGAGG
BfrI 569 CTTAAG C^TTAAG
1649 CTTAAG
BlnI 715 CCTAGG C^CTAGG
1443 CCTAGG
BlpI 32 GCTAAGC GC^T[ACGT]AGC
71 GCTGAGC
Bpu1102I 32 GCTAAGC GC^T[ACGT]AGC
71 GCTGAGC
BsaWI 52 TCCGGA [AT]^CCGG[AT]
547 TCCGGA
BseAI 52 TCCGGA T^CCGGA
547 TCCGGA
BsmAI 1394 GAGAC GTCTC(1/5)
1483 GTCTC
BspEI 52 TCCGGA T^CCGGA
547 TCCGGA
BstBI 43 TTCGAA TT^CGAA
1289 TTCGAA
CelII 32 GCTAAGC GC^T[ACGT]AGC
71 GCTGAGC
EarI 468 CTCTTC CTCTTC(1/4)
1140 GAAGAG
FauI 604 CCCGC CCCGC(4/6)
813 CCCGC
Ksp632I 468 CTCTTC CTCTTC(1/4)
1140 GAAGAG
MroI 52 TCCGGA T^CCGGA
547 TCCGGA
MslI 359 CATCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1407 CATGTATGTG
MspA1I 1340 CAGCTG C[AC]G^C[GT]G
1589 CAGCTG
NspV 43 TTCGAA TT^CGAA
1289 TTCGAA
PvuII 1340 CAGCTG CAG^CTG
1589 CAGCTG
SfaNI 705 GCATC GCATC(5/9)
1099 GCATC
SfuI 43 TTCGAA TT^CGAA
1289 TTCGAA
SmlI 569 CTTAAG C^T[CT][GA]AG
1649 CTTAAG
StuI 65 AGGCCT AGG^CCT
1533 AGGCCT
XmnI 398 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1793 GAATAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AocI AvaII
BanI BanII BbvI BfaI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Bsu36I Cac8I CfoI Cfr13I DdeI
Eco81I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboII MnlI MseI MspI MvaI
MwoI NlaIII NlaIV NspI PleI
RsaI Sau96I ScrFI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I