################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I EcoNI EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MseI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I TthHB8I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Alw44I 562 GTGCAC G^TGCAC AlwI 56 GATCC GGATC(4/5) AlwNI 639 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 596 AGCGCT AGC^GCT ApaLI 562 GTGCAC G^TGCAC AspI 166 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BglII 402 AGATCT A^GATCT BlpI 675 GCTAAGC GC^T[ACGT]AGC Bpu1102I 675 GCTAAGC GC^T[ACGT]AGC BseRI 748 GAGGAG GAGGAG(10/8) BsgI 575 CTGCAC GTGCAG(16/14) BsrFI 484 ACCGGC [GA]^CCGG[CT] BstEII 701 GGTGACC G^GT[ACGT]ACC BstPI 701 GGTGACC G^GT[ACGT]ACC CelII 675 GCTAAGC GC^T[ACGT]AGC CfoI 597 GCGC GCG^C Cfr10I 484 ACCGGC [GA]^CCGG[CT] EarI 305 CTCTTC CTCTTC(1/4) Eco47III 596 AGCGCT AGC^GCT EcoO65I 701 GGTGACC G^GT[ACGT]ACC EcoRI 42 GAATTC G^AATTC EcoT14I 198 CCAAGG C^C[AT][AT]GG HaeII 596 AGCGCT [GA]GCGC^[CT] HgaI 121 GCGTC GACGC(5/10) HhaI 597 GCGC GCG^C HinP1I 597 GCGC G^CGC Ksp632I 305 CTCTTC CTCTTC(1/4) NspI 710 GCATGT [GA]CATG^[CT] PflFI 166 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC SapI 304 GCTCTTC GCTCTTC(1/4) SmlI 270 CTCAAG C^T[CT][GA]AG StyI 198 CCAAGG C^C[AT][AT]GG Tth111I 166 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 61 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 42 GAATTC [GA]^AATT[CT] 929 GAATTT AocI 185 CCTCAGG CC^T[ACGT]AGG 696 CCTAAGG ApaI 691 GGGCCC GGGCC^C 809 GGGCCC ApoI 42 GAATTC [GA]^AATT[CT] 929 GAATTT BalI 195 TGGCCA TGG^CCA 417 TGGCCA BcnI 229 CCCGG CC^[GC]GG 475 CCCGG BpmI 804 CTCCAG CTGGAG(16/14) 771 CTGGAG BsaI 866 GAGACC GGTCTC(1/5) 262 GGTCTC BsmFI 233 GTCCC GGGAC(10/14) 435 GTCCC BstF5I 589 CATCC GGATG(2/0) 824 GGATG BstYI 55 AGATCC [GA]^GATC[CT] 402 AGATCT Bsu36I 185 CCTCAGG CC^T[ACGT]AGG 696 CCTAAGG DpnII 56 GATC ^GATC 403 GATC EaeI 195 TGGCCA [CT]^GGCC[GA] 417 TGGCCA Eco81I 185 CCTCAGG CC^T[ACGT]AGG 696 CCTAAGG FokI 589 CATCC GGATG(9/13) 824 GGATG HindIII 833 AAGCTT A^AGCTT 841 AAGCTT HinfI 170 GAGTC G^A[ACGT]TC 904 GAGTC MboI 56 GATC ^GATC 403 GATC MboII 65 GAAGA GAAGA(8/7) 306 TCTTC MflI 55 AGATCC [GA]^GATC[CT] 402 AGATCT MluNI 195 TGGCCA TGG^CCA 417 TGGCCA MscI 195 TGGCCA TGG^CCA 417 TGGCCA NciI 229 CCCGG CC^[GC]GG 475 CCCGG NdeII 56 GATC ^GATC 403 GATC PleI 170 GAGTC GAGTC(4/5) 904 GAGTC Sau3AI 56 GATC ^GATC 403 GATC Tsp509I 43 AATT ^AATT 930 AATT XcmI 428 CCAGGAAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 513 CCAAAGTTCTACTGG XhoII 55 AGATCC [GA]^GATC[CT] 402 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI AspHI AvaII BanII BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HpaII HphI ItaI MaeI MaeIII MnlI MspI MvaI MwoI NlaIII NlaIV PstI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI