##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco52I      EcoNI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HpaI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MroI        
MseI        MslI        MspA1I      MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       TthHB8I     Van91I      
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Alw44I        562  GTGCAC               G^TGCAC
AlwI           56  GATCC                GGATC(4/5)
AlwNI         639  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       596  AGCGCT               AGC^GCT
ApaLI         562  GTGCAC               G^TGCAC
AspI          166  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BglII         402  AGATCT               A^GATCT
BlpI          675  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      675  GCTAAGC              GC^T[ACGT]AGC
BseRI         748  GAGGAG               GAGGAG(10/8)
BsgI          575  CTGCAC               GTGCAG(16/14)
BsrFI         484  ACCGGC               [GA]^CCGG[CT]
BstEII        701  GGTGACC              G^GT[ACGT]ACC
BstPI         701  GGTGACC              G^GT[ACGT]ACC
CelII         675  GCTAAGC              GC^T[ACGT]AGC
CfoI          597  GCGC                 GCG^C
Cfr10I        484  ACCGGC               [GA]^CCGG[CT]
EarI          305  CTCTTC               CTCTTC(1/4)
Eco47III      596  AGCGCT               AGC^GCT
EcoO65I       701  GGTGACC              G^GT[ACGT]ACC
EcoRI          42  GAATTC               G^AATTC
EcoT14I       198  CCAAGG               C^C[AT][AT]GG
HaeII         596  AGCGCT               [GA]GCGC^[CT]
HgaI          121  GCGTC                GACGC(5/10)
HhaI          597  GCGC                 GCG^C
HinP1I        597  GCGC                 G^CGC
Ksp632I       305  CTCTTC               CTCTTC(1/4)
NspI          710  GCATGT               [GA]CATG^[CT]
PflFI         166  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SapI          304  GCTCTTC              GCTCTTC(1/4)
SmlI          270  CTCAAG               C^T[CT][GA]AG
StyI          198  CCAAGG               C^C[AT][AT]GG
Tth111I       166  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI           61  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcsI           42  GAATTC               [GA]^AATT[CT]
              929  GAATTT              
AocI          185  CCTCAGG              CC^T[ACGT]AGG
              696  CCTAAGG             
ApaI          691  GGGCCC               GGGCC^C
              809  GGGCCC              
ApoI           42  GAATTC               [GA]^AATT[CT]
              929  GAATTT              
BalI          195  TGGCCA               TGG^CCA
              417  TGGCCA              
BcnI          229  CCCGG                CC^[GC]GG
              475  CCCGG               
BpmI          804  CTCCAG               CTGGAG(16/14)
              771  CTGGAG              
BsaI          866  GAGACC               GGTCTC(1/5)
              262  GGTCTC              
BsmFI         233  GTCCC                GGGAC(10/14)
              435  GTCCC               
BstF5I        589  CATCC                GGATG(2/0)
              824  GGATG               
BstYI          55  AGATCC               [GA]^GATC[CT]
              402  AGATCT              
Bsu36I        185  CCTCAGG              CC^T[ACGT]AGG
              696  CCTAAGG             
DpnII          56  GATC                 ^GATC
              403  GATC                
EaeI          195  TGGCCA               [CT]^GGCC[GA]
              417  TGGCCA              
Eco81I        185  CCTCAGG              CC^T[ACGT]AGG
              696  CCTAAGG             
FokI          589  CATCC                GGATG(9/13)
              824  GGATG               
HindIII       833  AAGCTT               A^AGCTT
              841  AAGCTT              
HinfI         170  GAGTC                G^A[ACGT]TC
              904  GAGTC               
MboI           56  GATC                 ^GATC
              403  GATC                
MboII          65  GAAGA                GAAGA(8/7)
              306  TCTTC               
MflI           55  AGATCC               [GA]^GATC[CT]
              402  AGATCT              
MluNI         195  TGGCCA               TGG^CCA
              417  TGGCCA              
MscI          195  TGGCCA               TGG^CCA
              417  TGGCCA              
NciI          229  CCCGG                CC^[GC]GG
              475  CCCGG               
NdeII          56  GATC                 ^GATC
              403  GATC                
PleI          170  GAGTC                GAGTC(4/5)
              904  GAGTC               
Sau3AI         56  GATC                 ^GATC
              403  GATC                
Tsp509I        43  AATT                 ^AATT
              930  AATT                
XcmI          428  CCAGGAAGTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              513  CCAAAGTTCTACTGG     
XhoII          55  AGATCC               [GA]^GATC[CT]
              402  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AluI        AspHI       AvaII       BanII       
BbvI        BfaI        BmyI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstNI       Cac8I       Cfr13I      DdeI        
DraII       EcoO109I    EcoRII      Fnu4HI      HaeIII      
HapII       HpaII       HphI        ItaI        MaeI        
MaeIII      MnlI        MspI        MvaI        MwoI        
NlaIII      NlaIV       PstI        Sau96I      ScrFI       
SfaNI       SfcI        TseI        Tsp45I      TspRI