##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I EcoNI EcoRV EcoT22I FauI
FbaI FseI FspI Hin1I HincII
HindII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MseI MslI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I TthHB8I Van91I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Alw44I 562 GTGCAC G^TGCAC
AlwI 56 GATCC GGATC(4/5)
AlwNI 639 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 596 AGCGCT AGC^GCT
ApaLI 562 GTGCAC G^TGCAC
AspI 166 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglII 402 AGATCT A^GATCT
BlpI 675 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 675 GCTAAGC GC^T[ACGT]AGC
BseRI 748 GAGGAG GAGGAG(10/8)
BsgI 575 CTGCAC GTGCAG(16/14)
BsrFI 484 ACCGGC [GA]^CCGG[CT]
BstEII 701 GGTGACC G^GT[ACGT]ACC
BstPI 701 GGTGACC G^GT[ACGT]ACC
CelII 675 GCTAAGC GC^T[ACGT]AGC
CfoI 597 GCGC GCG^C
Cfr10I 484 ACCGGC [GA]^CCGG[CT]
EarI 305 CTCTTC CTCTTC(1/4)
Eco47III 596 AGCGCT AGC^GCT
EcoO65I 701 GGTGACC G^GT[ACGT]ACC
EcoRI 42 GAATTC G^AATTC
EcoT14I 198 CCAAGG C^C[AT][AT]GG
HaeII 596 AGCGCT [GA]GCGC^[CT]
HgaI 121 GCGTC GACGC(5/10)
HhaI 597 GCGC GCG^C
HinP1I 597 GCGC G^CGC
Ksp632I 305 CTCTTC CTCTTC(1/4)
NspI 710 GCATGT [GA]CATG^[CT]
PflFI 166 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 304 GCTCTTC GCTCTTC(1/4)
SmlI 270 CTCAAG C^T[CT][GA]AG
StyI 198 CCAAGG C^C[AT][AT]GG
Tth111I 166 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 61 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 42 GAATTC [GA]^AATT[CT]
929 GAATTT
AocI 185 CCTCAGG CC^T[ACGT]AGG
696 CCTAAGG
ApaI 691 GGGCCC GGGCC^C
809 GGGCCC
ApoI 42 GAATTC [GA]^AATT[CT]
929 GAATTT
BalI 195 TGGCCA TGG^CCA
417 TGGCCA
BcnI 229 CCCGG CC^[GC]GG
475 CCCGG
BpmI 804 CTCCAG CTGGAG(16/14)
771 CTGGAG
BsaI 866 GAGACC GGTCTC(1/5)
262 GGTCTC
BsmFI 233 GTCCC GGGAC(10/14)
435 GTCCC
BstF5I 589 CATCC GGATG(2/0)
824 GGATG
BstYI 55 AGATCC [GA]^GATC[CT]
402 AGATCT
Bsu36I 185 CCTCAGG CC^T[ACGT]AGG
696 CCTAAGG
DpnII 56 GATC ^GATC
403 GATC
EaeI 195 TGGCCA [CT]^GGCC[GA]
417 TGGCCA
Eco81I 185 CCTCAGG CC^T[ACGT]AGG
696 CCTAAGG
FokI 589 CATCC GGATG(9/13)
824 GGATG
HindIII 833 AAGCTT A^AGCTT
841 AAGCTT
HinfI 170 GAGTC G^A[ACGT]TC
904 GAGTC
MboI 56 GATC ^GATC
403 GATC
MboII 65 GAAGA GAAGA(8/7)
306 TCTTC
MflI 55 AGATCC [GA]^GATC[CT]
402 AGATCT
MluNI 195 TGGCCA TGG^CCA
417 TGGCCA
MscI 195 TGGCCA TGG^CCA
417 TGGCCA
NciI 229 CCCGG CC^[GC]GG
475 CCCGG
NdeII 56 GATC ^GATC
403 GATC
PleI 170 GAGTC GAGTC(4/5)
904 GAGTC
Sau3AI 56 GATC ^GATC
403 GATC
Tsp509I 43 AATT ^AATT
930 AATT
XcmI 428 CCAGGAAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
513 CCAAAGTTCTACTGG
XhoII 55 AGATCC [GA]^GATC[CT]
402 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BfaI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DraII EcoO109I EcoRII Fnu4HI HaeIII
HapII HpaII HphI ItaI MaeI
MaeIII MnlI MspI MvaI MwoI
NlaIII NlaIV PstI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I TspRI