##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AlwI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BspDI BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV FauI FbaI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XbaI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AciI 80 GCGG CCGC(-3/-1)
AflII 636 CTTAAG C^TTAAG
Alw44I 113 GTGCAC G^TGCAC
ApaLI 113 GTGCAC G^TGCAC
Asp700I 1253 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 345 CTCGAG C^[CT]CG[GA]G
BanII 969 GAGCTC G[GA]GC[CT]^C
BbsI 657 GTCTTC GAAGAC(2/6)
BfrI 636 CTTAAG C^TTAAG
BpuAI 657 GTCTTC GAAGAC(2/6)
BsaBI 676 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1089 GGTCTC GGTCTC(1/5)
BsaJI 1062 CCAAGG C^C[ACGT][ACGT]GG
BsgI 52 CTGCAC GTGCAG(16/14)
BsiYI 566 CCCCTTCTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 566 CCCCTTCTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 1129 GCATTC GAATGC(1/-1)
BsoBI 345 CTCGAG C^[CT]CG[GA]G
BssKI 277 CCTGG ^CC[ACGT]GG
BstAPI 98 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 277 CCTGG CC^[AT]GG
DpnII 172 GATC ^GATC
DraIII 649 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 912 GACAATCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1293 GAATTC G^AATTC
EcoRII 277 CCTGG ^CC[AT]GG
EcoT14I 1062 CCAAGG C^C[AT][AT]GG
EcoT22I 746 ATGCAT ATGCA^T
MaeIII 772 GTGAC ^GT[ACGT]AC
MamI 676 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 172 GATC ^GATC
MvaI 277 CCTGG CC^[AT]GG
NdeII 172 GATC ^GATC
NheI 83 GCTAGC G^CTAGC
NlaIV 988 GGAGCC GG[ACGT]^[ACGT]CC
NsiI 746 ATGCAT ATGCA^T
PaeR7I 345 CTCGAG C^TCGAG
SacI 969 GAGCTC GAGCT^C
Sau3AI 172 GATC ^GATC
ScaI 372 AGTACT AGT^ACT
ScrFI 277 CCTGG CC^[ACGT]GG
SfcI 1046 CTGTAG C^T[GA][CT]AG
SspI 948 AATATT AAT^ATT
StuI 515 AGGCCT AGG^CCT
StyI 1062 CCAAGG C^C[AT][AT]GG
TfiI 15 GAATC G^A[AT]TC
Tsp45I 772 GTGAC ^GT[GC]AC
XcmI 802 CCAATAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 345 CTCGAG C^TCGAG
XmnI 1253 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 183 GTAGAC GT^[AC][GT]AC
581 GTAGAC
AspHI 113 GTGCAC G[AT]GC[AT]^C
969 GAGCTC
BbvI 35 GCAGC GCAGC(8/12)
149 GCAGC
BmyI 113 GTGCAC G[AGT]GC[ACT]^C
969 GAGCTC
BsiHKAI 113 GTGCAC G[AT]GC[AT]^C
969 GAGCTC
BsmFI 362 GGGAC GGGAC(10/14)
338 GTCCC
Bsp1286I 113 GTGCAC G[AGT]GC[ACT]^C
969 GAGCTC
Bsp1407I 787 TGTACA T^GTACA
1070 TGTACA
BspHI 700 TCATGA T^CATGA
820 TCATGA
BsrGI 787 TGTACA T^GTACA
1070 TGTACA
Cfr13I 161 GGCCC G^G[ACGT]CC
1275 GGGCC
EarI 491 CTCTTC CTCTTC(1/4)
543 GAAGAG
HphI 222 GGTGA GGTGA(8/7)
857 GGTGA
Ksp632I 491 CTCTTC CTCTTC(1/4)
543 GAAGAG
MaeII 919 ACGT A^CGT
1077 ACGT
RcaI 700 TCATGA T^CATGA
820 TCATGA
Sau96I 161 GGCCC G^G[ACGT]CC
1275 GGGCC
SspBI 787 TGTACA T^GTACA
1070 TGTACA
TseI 35 GCAGC G^C[AT]GC
149 GCAGC
TspRI 651 CACTG CA[GC]TG[ACGT][ACGT]^
784 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
BfaI BsmAI BspLU11I BsrI Cac8I
DdeI DraI Fnu4HI HaeIII HinfI
ItaI MaeI MboII MnlI MseI
MwoI NlaIII NspI PleI RsaI
SfaNI SmlI TaqI Tru9I Tsp509I
TthHB8I