##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BcgI BciVI
BfrI BglI BlnI BsaWI BseAI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MluI MluNI MroI
MscI NarI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 714 GACGTC GACGT^C
AcyI 714 GACGTC G[GA]^CG[CT]C
AflIII 314 ACGTGT A^C[GA][CT]GT
Alw44I 1564 GTGCAC G^TGCAC
ApaLI 1564 GTGCAC G^TGCAC
AseI 1883 ATTAAT AT^TAAT
AsnI 1883 ATTAAT AT^TAAT
AvaI 1544 CTCGGG C^[CT]CG[GA]G
BanI 176 GGCACC G^G[CT][GA]CC
BbrPI 313 CACGTG CAC^GTG
BbsI 999 GAAGAC GAAGAC(2/6)
BbvCI 1813 GCTGAGG CCTCAGC(-5/-2)
BglII 1337 AGATCT A^GATCT
BlpI 1241 GCTGAGC GC^T[ACGT]AGC
BpmI 466 CTCCAG CTGGAG(16/14)
Bpu1102I 1241 GCTGAGC GC^T[ACGT]AGC
BpuAI 999 GAAGAC GAAGAC(2/6)
BsaAI 313 CACGTG [CT]AC^GT[GA]
BsaBI 822 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 714 GACGTC G[GA]^CG[CT]C
BsaI 948 GAGACC GGTCTC(1/5)
BsgI 79 GTGCAG GTGCAG(16/14)
BsiEI 294 CGACCG CG[GA][CT]^CG
BsiWI 989 CGTACG C^GTACG
BsmI 1461 GCATTC GAATGC(1/-1)
BsoBI 1544 CTCGGG C^[CT]CG[GA]G
BspHI 413 TCATGA T^CATGA
BsrDI 1331 CATTGC GCAATG(2/0)
BstAPI 1096 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1309 CCAGGCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1241 GCTGAGC GC^T[ACGT]AGC
CfoI 2 GCGC GCG^C
DraI 1410 TTTAAA TTT^AAA
DraII 379 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 714 GACGTCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 600 CCATGG C^C[GA][CT]GG
EcoO109I 379 GGGACCT [GA]G^G[ACGT]CC[CT]
HhaI 2 GCGC GCG^C
Hin1I 714 GACGTC G[GA]^CG[CT]C
HinP1I 2 GCGC G^CGC
MamI 822 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1208 CAATTG C^AATTG
MunI 1208 CAATTG C^AATTG
NaeI 61 GCCGGC GCC^GGC
NcoI 600 CCATGG C^CATGG
NdeI 1353 CATATG CA^TATG
NgoMIV 61 GCCGGC G^CCGGC
PflMI 1071 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 975 GACTC GAGTC(4/5)
PmaCI 313 CACGTG CAC^GTG
PmlI 313 CACGTG CAC^GTG
PpuMI 379 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1206 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1883 ATTAAT AT^TAAT
PvuII 861 CAGCTG CAG^CTG
RcaI 413 TCATGA T^CATGA
SexAI 1395 ACCAGGT A^CC[AT]GGT
SgrAI 60 CGCCGGCG C[GA]^CCGG[CT]G
TfiI 1188 GATTC G^A[AT]TC
Van91I 1071 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1162 GTAGAC GT^[AC][GT]AC
1506 GTCTAC
AccII 1 CGCG CG^CG
3 CGCG
AfaI 990 GTAC GT^AC
1045 GTAC
AlwI 605 GGATC GGATC(4/5)
1548 GGATC
AlwNI 1097 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1784 CAGCACCTG
Asp700I 405 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
999 GAAGACATTC
BanII 447 GAGCCC G[GA]GC[CT]^C
983 GGGCTC
BclI 1349 TGATCA T^GATCA
1828 TGATCA
BcnI 297 CCGGG CC^[GC]GG
979 CCGGG
BfaI 115 CTAG C^TAG
473 CTAG
BspMI 1092 ACCTGC ACCTGC(4/8)
1788 ACCTGC
BsrBI 69 GAGCGG CCGCTC(-3/-3)
499 GAGCGG
BsrFI 61 GCCGGC [GA]^CCGG[CT]
587 ACCGGC
BstUI 1 CGCG CG^CG
3 CGCG
BstYI 1337 AGATCT [GA]^GATC[CT]
1548 GGATCT
Cac8I 61 GCCGGC GC[ACGT]^[ACGT]GC
1500 GCAAGC
Cfr10I 61 GCCGGC [GA]^CCGG[CT]
587 ACCGGC
EcoRI 334 GAATTC G^AATTC
520 GAATTC
EcoT14I 600 CCATGG C^C[AT][AT]GG
1201 CCAAGG
FauI 65 GCGGG CCCGC(4/6)
74 GCGGG
FbaI 1349 TGATCA T^GATCA
1828 TGATCA
HinfI 975 GACTC G^A[ACGT]TC
1188 GATTC
MaeI 115 CTAG C^TAG
473 CTAG
MflI 1337 AGATCT [GA]^GATC[CT]
1548 GGATCT
MvnI 1 CGCG CG^CG
3 CGCG
NciI 297 CCGGG CC^[GC]GG
979 CCGGG
PstI 1094 CTGCAG CTGCA^G
1103 CTGCAG
RsaI 990 GTAC GT^AC
1045 GTAC
SfcI 1094 CTGCAG C^T[GA][CT]AG
1103 CTGCAG
SmlI 1443 CTCAAG C^T[CT][GA]AG
1834 CTTGAG
StuI 248 AGGCCT AGG^CCT
407 AGGCCT
StyI 600 CCATGG C^C[AT][AT]GG
1201 CCAAGG
Tsp45I 955 GTCAC ^GT[GC]AC
1019 GTGAC
XhoII 1337 AGATCT [GA]^GATC[CT]
1548 GGATCT
XmnI 405 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
999 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AluI ApoI AspHI
AvaII BbvI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp509I TspRI